MetaCyc Pathway: biotin biosynthesis I in Cupriavidus basilensis FW507-4G11

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Reactions and Genes Valeric (C)
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a malonyl-[acp] + S-adenosyl-L-methionine→a malonyl-[acp] methyl ester + S-adenosyl-L-homocysteine (EC 2.1.1.197)
No genes
Beta-ketoacyl-acyl-carrier-protein synthase II:
a malonyl-[acp] + a malonyl-[acp] methyl ester + H+→a 3-oxoglutaryl-[acp] methyl ester + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
RR42_RS14090 N.D.
RR42_RS31185 -0.0
RR42_RS31200 -0.1
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxoglutaryl-[acp] methyl ester + NADPH + H+→a (3R)-3-hydroxyglutaryl-[acp] methyl ester + NADP+ (EC 1.1.1.100)
RR42_RS04765 -1.8
RR42_RS08710 -0.4
RR42_RS09330 -0.2
RR42_RS10255 +0.0
RR42_RS10315 +0.1
RR42_RS10320 +0.1
RR42_RS11070 -0.5
RR42_RS11550 -0.8
RR42_RS13675 -0.1
RR42_RS13690 +0.2
RR42_RS14100 N.D.
RR42_RS16365 -0.1
RR42_RS16375 +0.5
RR42_RS16800 +0.1
RR42_RS17660 +0.0
RR42_RS21765 +0.8
RR42_RS21790 -0.2
RR42_RS21865 +0.0
RR42_RS22390 -0.0
RR42_RS22710 +0.4
RR42_RS22740 -0.3
RR42_RS22810 +0.3
RR42_RS23420 +0.4
RR42_RS23690 -0.3
RR42_RS24195 -0.2
RR42_RS25125 -0.1
RR42_RS25495 -0.2
RR42_RS28045 -0.3
RR42_RS28985 +0.2
RR42_RS29305 +0.0
RR42_RS31190 +0.3
RR42_RS31670 -0.3
RR42_RS31820 -0.6
RR42_RS31835 -0.2
RR42_RS31900 +0.5
RR42_RS33070 +0.8
RR42_RS33710 +0.1
RR42_RS34680 +0.5
RR42_RS35910 -0.0
RR42_RS36415 +0.1
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxyglutaryl-[acp] methyl ester→a (2E)-enoylglutaryl-[acp] methyl ester + H2O (EC 4.2.1.59)
RR42_RS11820 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-enoylglutaryl-[acp] methyl ester + NADH + H+→a glutaryl-[acp] methyl ester + NAD+ (EC 1.3.1.9)
RR42_RS03815 -0.5
RR42_RS10070 +0.0
RR42_RS10225 -0.5
RR42_RS13390 -0.1
RR42_RS25240 -0.2
RR42_RS31520 -0.3
RR42_RS31940 -0.7
RR42_RS36330 -0.4
Beta-ketoacyl-acyl-carrier-protein synthase II:
a glutaryl-[acp] methyl ester + a malonyl-[acp] + H+→a 3-oxopimeloyl-[acp] methyl ester + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
RR42_RS14090 N.D.
RR42_RS31185 -0.0
RR42_RS31200 -0.1
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxopimeloyl-[acp] methyl ester + NADPH + H+→a (3R)-3-hydroxypimeloyl-[acp] methyl ester + NADP+ (EC 1.1.1.100)
RR42_RS04765 -1.8
RR42_RS08710 -0.4
RR42_RS09330 -0.2
RR42_RS10255 +0.0
RR42_RS10315 +0.1
RR42_RS10320 +0.1
RR42_RS11070 -0.5
RR42_RS11550 -0.8
RR42_RS13675 -0.1
RR42_RS13690 +0.2
RR42_RS14100 N.D.
RR42_RS16365 -0.1
RR42_RS16375 +0.5
RR42_RS16800 +0.1
RR42_RS17660 +0.0
RR42_RS21765 +0.8
RR42_RS21790 -0.2
RR42_RS21865 +0.0
RR42_RS22390 -0.0
RR42_RS22710 +0.4
RR42_RS22740 -0.3
RR42_RS22810 +0.3
RR42_RS23420 +0.4
RR42_RS23690 -0.3
RR42_RS24195 -0.2
RR42_RS25125 -0.1
RR42_RS25495 -0.2
RR42_RS28045 -0.3
RR42_RS28985 +0.2
RR42_RS29305 +0.0
RR42_RS31190 +0.3
RR42_RS31670 -0.3
RR42_RS31820 -0.6
RR42_RS31835 -0.2
RR42_RS31900 +0.5
RR42_RS33070 +0.8
RR42_RS33710 +0.1
RR42_RS34680 +0.5
RR42_RS35910 -0.0
RR42_RS36415 +0.1
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxypimeloyl-[acp] methyl ester→a (2E)-enoylpimeloyl-[acp] methyl ester + H2O (EC 4.2.1.59)
RR42_RS11820 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-enoylpimeloyl-[acp] methyl ester + NADH + H+→a pimeloyl-[acp] methyl ester + NAD+ (EC 1.3.1.9)
RR42_RS03815 -0.5
RR42_RS10070 +0.0
RR42_RS10225 -0.5
RR42_RS13390 -0.1
RR42_RS25240 -0.2
RR42_RS31520 -0.3
RR42_RS31940 -0.7
RR42_RS36330 -0.4
a pimeloyl-[acp] methyl ester + H2O→a pimeloyl-[acp] + methanol + H+ (EC 3.1.1.85)
No genes
8-amino-7-oxononanoate synthase:
a pimeloyl-[acp] + L-alanine + H+→(8S)-8-amino-7-oxononanoate + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.47)
RR42_RS00930 -1.2
RR42_RS10140 -1.3
RR42_RS29265 -0.0
Adenosylmethionine--8-amino-7-oxononanoate transaminase:
(8S)-8-amino-7-oxononanoate + S-adenosyl-L-methionine→(7R,8S)-diaminononanoate + S-adenosyl-4-methylsulfanyl-2-oxobutanoate (EC 2.6.1.62)
RR42_RS00925 -1.8
RR42_RS26850 +0.1
RR42_RS27385 -0.1
RR42_RS27405 -0.0
Dethiobiotin synthase:
(7R,8S)-diaminononanoate + ATP + CO2→dethiobiotin + ADP + 3 H+ + phosphate (EC 6.3.3.3)
RR42_RS00935 N.D.
Biotin synthase:
dethiobiotin + H+ + 2 a reduced [2Fe-2S] ferredoxin + S-adenosyl-L-methionine + a sulfurated [sulfur carrier]→4,5-secobiotin + 5'-deoxyadenosine + L-methionine + 2 an oxidized [2Fe-2S] ferredoxin + an unsulfurated [sulfur carrier] (EC 2.8.1.6)
RR42_RS00940 -2.7
Biotin synthase:
4,5-secobiotin + S-adenosyl-L-methionine→biotin + 5'-deoxyadenosine + L-methionine + H+ (EC 2.8.1.6)
RR42_RS00940 -2.7

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information