Experiment set5IT003 for Cupriavidus basilensis FW507-4G11
R2A with Dimethyl Sulfoxide 5 vol%
Group: stressMedia: R2A + Dimethyl Sulfoxide (5 vol%)
Culturing: cupriavidus_4G11_ML11, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
Growth: about 3.6 generations
By: Mark on 5/13/2015
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate
Specific Phenotypes
For 3 genes in this experiment
For stress Dimethyl Sulfoxide in Cupriavidus basilensis FW507-4G11
For stress Dimethyl Sulfoxide across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Folate Biosynthesis | 1 |
| YcfH | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Purine metabolism
- Pyrimidine metabolism
- Nicotinate and nicotinamide metabolism
- Biosynthesis of alkaloids derived from histidine and purine
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| NAD salvage pathway III (to nicotinamide riboside) | 3 | 2 | 1 |
| UTP and CTP dephosphorylation I | 7 | 5 | 2 |
| purine nucleotides degradation II (aerobic) | 11 | 8 | 3 |
| inosine 5'-phosphate degradation | 4 | 3 | 1 |
| guanosine nucleotides degradation II | 4 | 3 | 1 |
| guanosine nucleotides degradation III | 4 | 3 | 1 |
| adenosine nucleotides degradation I | 8 | 5 | 2 |
| purine nucleotides degradation I (plants) | 12 | 7 | 3 |
| guanosine nucleotides degradation I | 4 | 2 | 1 |
| adenosine nucleotides degradation II | 5 | 4 | 1 |
| superpathway of purines degradation in plants | 18 | 11 | 3 |
| superpathway of guanosine nucleotides degradation (plants) | 6 | 3 | 1 |
| ureide biosynthesis | 7 | 5 | 1 |
| tunicamycin biosynthesis | 9 | 1 | 1 |
| NAD salvage (plants) | 11 | 6 | 1 |