Experiment set4IT073 for Cupriavidus basilensis FW507-4G11

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R2A with Polymyxin B sulfate 0.005 mg/ml

Group: stress
Media: R2A + Polymyxin B sulfate (0.005 mg/ml)
Culturing: cupriavidus_4G11_ML11, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
Growth: about 3.2 generations
By: Mark on 05/13/2015
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate
Growth plate: plate3 D2

Specific Phenotypes

For 24 genes in this experiment

For stress Polymyxin B sulfate in Cupriavidus basilensis FW507-4G11

For stress Polymyxin B sulfate across organisms

SEED Subsystems

Subsystem #Specific
Bacterial Cell Division 2
Bacterial Cytoskeleton 2
Multidrug Resistance Efflux Pumps 2
Arginine and Ornithine Degradation 1
Carotenoids 1
DNA repair, bacterial UvrD and related helicases 1
Lipid A modifications 1
Methionine Biosynthesis 1
Methionine Degradation 1
Multidrug efflux pump in Campylobacter jejuni (CmeABC operon) 1
Orphan regulatory proteins 1
Periplasmic disulfide interchange 1
Quorum Sensing: Autoinducer-2 Synthesis 1
Ton and Tol transport systems 1
tRNA processing 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
S-adenosyl-L-methionine biosynthesis 1 1 1
diploterol biosynthesis 2 1 1
pseudouridine degradation 2 1 1
S-adenosyl-L-methionine salvage II 3 3 1
L-methionine degradation I (to L-homocysteine) 3 2 1
ethene biosynthesis I (plants) 3 1 1
S-adenosyl-L-methionine salvage I 4 3 1
2'-deoxymugineic acid phytosiderophore biosynthesis 4 1 1
superpathway of L-cysteine biosynthesis (mammalian) 5 2 1
L-cysteine biosynthesis VI (reverse transsulfuration) 7 3 1
superpathway of S-adenosyl-L-methionine biosynthesis 9 7 1
L-methionine salvage cycle III 11 3 1
L-methionine salvage cycle II (plants) 11 2 1
L-methionine salvage cycle I (bacteria and plants) 12 4 1
hopanoid biosynthesis (bacteria) 14 4 1
superpathway of L-methionine salvage and degradation 16 7 1
aspartate superpathway 25 23 1