Experiment set3IT021 for Cupriavidus basilensis FW507-4G11

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L-Citrulline nitrogen source

Group: nitrogen source
Media: RCH2_defined_noNitrogen + L-Citrulline (20 mM), pH=7
Culturing: cupriavidus_4G11_ML11, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
By: Jayashree on 3/3/2015
Media components: 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM Sodium D,L-Lactate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 4 genes in this experiment

For nitrogen source L-Citrulline in Cupriavidus basilensis FW507-4G11

For nitrogen source L-Citrulline across organisms

SEED Subsystems

Subsystem #Specific
Arginine and Ornithine Degradation 2
Copper homeostasis: copper tolerance 1
Dimethylarginine metabolism 1
Phosphate metabolism 1
Proline, 4-hydroxyproline uptake and utilization 1
Respiratory dehydrogenases 1 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-ornithine degradation I (L-proline biosynthesis) 1 1 1
L-proline degradation I 3 3 2
proline to cytochrome bo oxidase electron transfer 2 2 1
L-proline biosynthesis II (from arginine) 2 1 1
L-arginine degradation VII (arginase 3 pathway) 2 1 1
L-arginine degradation I (arginase pathway) 3 1 1
ethene biosynthesis II (microbes) 4 1 1
(5R)-carbapenem carboxylate biosynthesis 6 1 1
L-Nδ-acetylornithine biosynthesis 7 4 1
L-citrulline biosynthesis 8 6 1
superpathway of L-citrulline metabolism 12 8 1