Experiment set2IT084 for Cupriavidus basilensis FW507-4G11

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L-Lysine nitrogen source

Group: nitrogen source
Media: RCH2_defined_noNitrogen + L-Lysine (20 mM), pH=7
Culturing: cupriavidus_4G11_ML11, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
By: Jayashree on 3/3/2015
Media components: 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM Sodium D,L-Lactate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 6 genes in this experiment

For nitrogen source L-Lysine in Cupriavidus basilensis FW507-4G11

For nitrogen source L-Lysine across organisms

SEED Subsystems

Subsystem #Specific
Chorismate Synthesis 1
Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) 1
Glycine cleavage system 1
Orphan regulatory proteins 1
Quinate degradation 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
gallate biosynthesis 3 2 1
quinate degradation II 3 1 1
quinate degradation I 3 1 1
chorismate biosynthesis from 3-dehydroquinate 5 5 1
chorismate biosynthesis I 7 7 1
superpathway of L-tyrosine biosynthesis 10 10 1
superpathway of L-phenylalanine biosynthesis 10 10 1
chorismate biosynthesis II (archaea) 12 8 1
superpathway of L-tryptophan biosynthesis 13 13 1
superpathway of aromatic amino acid biosynthesis 18 18 1
superpathway of aromatic compound degradation via 3-oxoadipate 35 25 1
superpathway of aromatic compound degradation via 2-hydroxypentadienoate 42 21 1
superpathway of chorismate metabolism 59 45 1