Experiment set1IT012 for Cupriavidus basilensis FW507-4G11
R2A with Sodium nitrate 200 mM
Group: stressMedia: R2A + Sodium nitrate (200 mM)
Culturing: cupriavidus_4G11_ML11, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Mark on 3/3/2015
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate
Growth plate: 1174 A7,A8
Specific Phenotypes
For 8 genes in this experiment
For stress Sodium nitrate in Cupriavidus basilensis FW507-4G11
For stress Sodium nitrate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Folate Biosynthesis | 1 |
| LMPTP YfkJ cluster | 1 |
| tRNA processing | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Ascorbate and aldarate metabolism
- Ubiquinone and menaquinone biosynthesis
- Purine metabolism
- Pyrimidine metabolism
- Nucleotide sugars metabolism
- Glycosaminoglycan degradation
- Nicotinate and nicotinamide metabolism
- Biosynthesis of alkaloids derived from histidine and purine
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| NAD salvage pathway III (to nicotinamide riboside) | 3 | 2 | 1 |
| UTP and CTP dephosphorylation I | 7 | 5 | 2 |
| purine nucleotides degradation II (aerobic) | 11 | 8 | 3 |
| guanosine nucleotides degradation II | 4 | 3 | 1 |
| guanosine nucleotides degradation III | 4 | 3 | 1 |
| inosine 5'-phosphate degradation | 4 | 3 | 1 |
| adenosine nucleotides degradation I | 8 | 5 | 2 |
| purine nucleotides degradation I (plants) | 12 | 7 | 3 |
| guanosine nucleotides degradation I | 4 | 2 | 1 |
| adenosine nucleotides degradation II | 5 | 4 | 1 |
| superpathway of purines degradation in plants | 18 | 11 | 3 |
| superpathway of guanosine nucleotides degradation (plants) | 6 | 3 | 1 |
| ureide biosynthesis | 7 | 5 | 1 |
| tunicamycin biosynthesis | 9 | 1 | 1 |
| NAD salvage (plants) | 11 | 6 | 1 |