Experiment set1IT003 for Cupriavidus basilensis FW507-4G11

R2A with copper (II) chloride dihydrate 0.25 mM

Group: stress
Media: R2A + copper (II) chloride dihydrate (0.25 mM)
Culturing: cupriavidus_4G11_ML11, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Mark on 03/03/2015
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate
Growth plate: 1173 B7,B8

Specific Phenotypes

For 17 genes in this experiment

For stress copper (II) chloride dihydrate in Cupriavidus basilensis FW507-4G11

For stress copper (II) chloride dihydrate across organisms

SEED Subsystems

Subsystem	#Specific
Copper homeostasis	2
2-phosphoglycolate salvage	1
Bacterial RNA-metabolizing Zn-dependent hydrolases	1
Cobalt-zinc-cadmium resistance	1
Conserved gene cluster associated with Met-tRNA formyltransferase	1
Glutathione-regulated potassium-efflux system and associated functions	1
Periplasmic disulfide interchange	1
Potassium homeostasis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Ascorbate and aldarate metabolism
• Ubiquinone and menaquinone biosynthesis
• Tryptophan metabolism
• Nucleotide sugars metabolism
• Glycosaminoglycan degradation
• Glyoxylate and dicarboxylate metabolism
• Biosynthesis of siderophore group nonribosomal peptides

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
manganese oxidation II	2	1	1
peptidoglycan recycling II	10	7	2
photorespiration I	9	6	1
photorespiration III	9	6	1
photorespiration II	10	6	1
peptidoglycan recycling I	14	10	1