Experiment set1IT001 for Cupriavidus basilensis FW507-4G11

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R2A with Nickel (II) chloride hexahydrate 0.35 mM

Group: stress
Media: R2A + Nickel (II) chloride hexahydrate (0.35 mM)
Culturing: cupriavidus_4G11_ML11, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Mark on 3/3/2015
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate
Growth plate: 1173 A5,A6

Specific Phenotypes

For 17 genes in this experiment

For stress Nickel (II) chloride hexahydrate in Cupriavidus basilensis FW507-4G11

For stress Nickel (II) chloride hexahydrate across organisms

SEED Subsystems

Subsystem #Specific
Thiamin biosynthesis 3
Hydantoin metabolism 2
Bacterial Chemotaxis 1
Bacterial RNA-metabolizing Zn-dependent hydrolases 1
Chorismate Synthesis 1
Cobalt-zinc-cadmium resistance 1
Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) 1
Flagellar motility 1
Iron acquisition in Vibrio 1
Oxidative stress 1
Quinate degradation 1
Transport of Iron 1
Two-component regulatory systems in Campylobacter 1
Widespread colonization island 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
gallate biosynthesis 3 2 1
quinate degradation I 3 1 1
quinate degradation II 3 1 1
chorismate biosynthesis from 3-dehydroquinate 5 5 1
creatinine degradation II 5 3 1
chorismate biosynthesis I 7 7 1
superpathway of L-phenylalanine biosynthesis 10 10 1
superpathway of L-tyrosine biosynthesis 10 10 1
chorismate biosynthesis II (archaea) 12 8 1
superpathway of L-tryptophan biosynthesis 13 13 1
superpathway of aromatic amino acid biosynthesis 18 18 1
tRNA charging 21 20 1
superpathway of aromatic compound degradation via 3-oxoadipate 35 25 1
superpathway of aromatic compound degradation via 2-hydroxypentadienoate 42 21 1
superpathway of chorismate metabolism 59 45 1