Experiment set13S586 for Methanococcus maripaludis S2

Compare to:

McCas_formate_complete 5-Fluorouracil 4.16666666666667E-05 mM

Group: stress
Media: McCas_formate_complete + 5-fluorouracil (4.17e-05 mM), pH=7
Culturing: Methanococcus_S2_ML1, 96 deep-well microplate; 1.0 mL volume, Anaerobic, at 37 (C)
By: Leslie Day on 9-Feb
Media components: 2.5 g/L Sodium bicarbonate, 10.5 g/L Sodium Chloride, 1 g/L Ammonium chloride, 0.14 g/L Potassium phosphate dibasic, 0.0095 g/L Iron (II) chloride tetrahydrate, 0.001 g/L resazurin sodium salt, 0.84 g/L Sodium acetate, 13.6 g/L Sodium Formate, 2 g/L casamino acids, 0.5 g/L Dithiothreitol, 0.001 g/L Sodium sulfide nonahydrate, MOPS_2M_pH7 (0.002 M 3-(N-morpholino)propanesulfonic acid, 0.0005 M Sodium Hydroxide), Mc_general_salts_SO4free (0.335 g/L Potassium Chloride, 5.6 g/L Magnesium chloride hexahydrate, 0.105 g/L Calcium chloride), Mc_trace_minerals_SO4free (0.021 g/L Trisodium citrate dihydrate, 0.005 g/L Manganese (II) chloride tetrahydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc chloride, 0.0001 g/L copper (II) chloride dihydrate, 0.0001 g/L Aluminum chloride hydrate, 0.0001 g/L Boric Acid, 0.001 g/L Sodium Molybdate Dihydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.002 g/L Sodium selenate, 0.0001 g/L vanadium chloride, 3.3e-05 g/L Sodium tungstate dihydrate), Mc_vitamins (2e-05 g/L biotin, 2e-05 g/L Folic Acid, 0.0001 g/L Pyridoxine HCl, 5e-05 g/L Thiamine HCl, 5e-05 g/L Riboflavin, 5e-05 g/L Nicotinic Acid, 5e-05 g/L calcium pantothenate, 1e-06 g/L Cyanocobalamin, 5e-05 g/L 4-Aminobenzoic acid, 5e-05 g/L Lipoic acid)

Specific Phenotypes

For 2 genes in this experiment

For stress 5-fluorouracil in Methanococcus maripaludis S2

For stress 5-fluorouracil across organisms

SEED Subsystems

Subsystem #Specific
De Novo Pyrimidine Synthesis 2
LMPTP YwlE cluster 1
Pyrimidine utilization 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
pyrimidine nucleobases salvage I 1 1 1
pyrimidine nucleobases salvage II 2 2 1
superpathway of pyrimidine nucleobases salvage 4 4 1
superpathway of pyrimidine ribonucleosides salvage 10 6 1