Experiment set11S273 for Methanococcus maripaludis S2

McCas_H2_complete 5-Fluorouracil 0.00833333333333333 mM

Group: stress
Media: McCas_H2_complete + 5-fluorouracil (0.008333333 mM), pH=7
Culturing: Methanococcus_S2_ML1, 96 deep-well microplate; 1.0 mL volume, Anaerobic, at 37 (C)
By: Leslie Day on 9-Feb
Media components: 22 g/L Sodium Chloride, 2.5 g/L Sodium bicarbonate, 1 g/L Ammonium chloride, 0.14 g/L Potassium phosphate dibasic, 0.0095 g/L Iron (II) chloride tetrahydrate, 0.001 g/L resazurin sodium salt, 0.5 g/L Dithiothreitol, 0.001 g/L Sodium sulfide nonahydrate, 2 g/L casamino acids, 0.84 g/L Sodium acetate, MOPS_2M_pH7 (0.002 M 3-(N-morpholino)propanesulfonic acid, 0.0005 M Sodium Hydroxide), Mc_general_salts_SO4free (0.335 g/L Potassium Chloride, 5.6 g/L Magnesium chloride hexahydrate, 0.105 g/L Calcium chloride), Mc_trace_minerals_SO4free (0.021 g/L Trisodium citrate dihydrate, 0.005 g/L Manganese (II) chloride tetrahydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc chloride, 0.0001 g/L copper (II) chloride dihydrate, 0.0001 g/L Aluminum chloride hydrate, 0.0001 g/L Boric Acid, 0.001 g/L Sodium Molybdate Dihydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.002 g/L Sodium selenate, 0.0001 g/L vanadium chloride, 3.3e-05 g/L Sodium tungstate dihydrate), Mc_vitamins (2e-05 g/L biotin, 2e-05 g/L Folic Acid, 0.0001 g/L Pyridoxine HCl, 5e-05 g/L Thiamine HCl, 5e-05 g/L Riboflavin, 5e-05 g/L Nicotinic Acid, 5e-05 g/L calcium pantothenate, 1e-06 g/L Cyanocobalamin, 5e-05 g/L 4-Aminobenzoic acid, 5e-05 g/L Lipoic acid)

Specific Phenotypes

For 3 genes in this experiment

For stress 5-fluorouracil in Methanococcus maripaludis S2

For stress 5-fluorouracil across organisms

SEED Subsystems

Subsystem	#Specific
De Novo Pyrimidine Synthesis	2
Folate Biosynthesis	1
LMPTP YwlE cluster	1
Pyrimidine utilization	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pyrimidine metabolism
• Purine metabolism
• Nicotinate and nicotinamide metabolism
• Biosynthesis of alkaloids derived from histidine and purine

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
pyrimidine nucleobases salvage I	1	1	1
pyrimidine nucleobases salvage II	2	2	1
NAD salvage pathway III (to nicotinamide riboside)	3	1	1
UTP and CTP dephosphorylation I	7	5	2
purine nucleotides degradation II (aerobic)	11	6	3
superpathway of pyrimidine nucleobases salvage	4	4	1
inosine 5'-phosphate degradation	4	2	1
guanosine nucleotides degradation III	4	2	1
adenosine nucleotides degradation I	8	3	2
purine nucleotides degradation I (plants)	12	4	3
guanosine nucleotides degradation I	4	1	1
guanosine nucleotides degradation II	4	1	1
adenosine nucleotides degradation II	5	2	1
superpathway of purines degradation in plants	18	4	3
superpathway of guanosine nucleotides degradation (plants)	6	1	1
ureide biosynthesis	7	2	1
tunicamycin biosynthesis	9	1	1
superpathway of pyrimidine ribonucleosides salvage	10	6	1
NAD salvage (plants)	11	1	1