Experiment set10IT076 for Cupriavidus basilensis FW507-4G11
Disodium Glutarate carbon source
Group: carbon sourceMedia: MOPS minimal media_noCarbon + Disodium Glutarate (10 mM)
Culturing: cupriavidus_4G11_ML11_JBEI, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 2.0 generations
By: Allie Pearson on 08/26/2019
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate
Specific Phenotypes
For 4 genes in this experiment
For carbon source Disodium Glutarate in Cupriavidus basilensis FW507-4G11
For carbon source Disodium Glutarate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Homogentisate pathway of aromatic compound degradation | 1 |
| Serine-glyoxylate cycle | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| glyoxylate cycle | 6 | 6 | 1 |
| superpathway of glyoxylate bypass and TCA | 12 | 11 | 1 |
| superpathway of glyoxylate cycle and fatty acid degradation | 14 | 11 | 1 |
| superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass | 26 | 23 | 1 |