Experiment set10IT051 for Cupriavidus basilensis FW507-4G11
Octanoic acid carbon source
Group: carbon sourceMedia: MOPS minimal media_noCarbon + Octanoic acid (10 mM)
Culturing: cupriavidus_4G11_ML11_JBEI, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 3.5 generations
By: Allie Pearson on 8/26/19
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate
Specific Phenotypes
For 9 genes in this experiment
For carbon source Octanoic acid in Cupriavidus basilensis FW507-4G11
For carbon source Octanoic acid across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria | 2 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| cellulose biosynthesis | 1 | 1 | 1 |
| β-alanine biosynthesis II | 6 | 5 | 1 |
| methylthiopropanoate degradation I (cleavage) | 6 | 2 | 1 |
| superpathway of dimethylsulfoniopropanoate degradation | 8 | 2 | 1 |
| valproate β-oxidation | 9 | 6 | 1 |
| superpathway of coenzyme A biosynthesis II (plants) | 10 | 9 | 1 |
| oleate β-oxidation | 35 | 29 | 1 |