Experiment set10IT048 for Cupriavidus basilensis FW507-4G11
Valeric acid carbon source
Group: carbon sourceMedia: MOPS minimal media_noCarbon + Valeric acid (10 mM)
Culturing: cupriavidus_4G11_ML11_JBEI, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 2.8 generations
By: Allie Pearson on 8/26/19
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate
Specific Phenotypes
For 1 genes in this experiment
For carbon source Valeric acid in Cupriavidus basilensis FW507-4G11
For carbon source Valeric acid across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Biotin biosynthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| 8-amino-7-oxononanoate biosynthesis III | 2 | 2 | 1 |
| 8-amino-7-oxononanoate biosynthesis II | 2 | 1 | 1 |
| 8-amino-7-oxononanoate biosynthesis IV | 5 | 4 | 1 |
| biotin biosynthesis II | 6 | 5 | 1 |
| 8-amino-7-oxononanoate biosynthesis I | 11 | 9 | 1 |
| biotin biosynthesis I | 15 | 13 | 1 |