Experiment: m.b. L-Lysine 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2578 and Dshi_2579 are separated by 143 nucleotides Dshi_2579 and Dshi_2580 are separated by 165 nucleotides
Dshi_2578: Dshi_2578 - phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (RefSeq), at 2,743,867 to 2,744,589
_2578
Dshi_2579: Dshi_2579 - hypothetical protein (RefSeq), at 2,744,733 to 2,745,137
_2579
Dshi_2580: Dshi_2580 - ATP-dependent helicase HrpB (RefSeq), at 2,745,303 to 2,747,807
_2580
Position (kb)
2744
2745
2746 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2743.741 kb on + strand at 2743.855 kb on + strand at 2744.681 kb on - strand at 2744.810 kb on + strand, within Dshi_2579 at 2744.825 kb on + strand, within Dshi_2579 at 2744.833 kb on - strand, within Dshi_2579 at 2744.833 kb on - strand, within Dshi_2579 at 2744.833 kb on - strand, within Dshi_2579 at 2744.942 kb on - strand, within Dshi_2579 at 2744.942 kb on - strand, within Dshi_2579 at 2744.945 kb on + strand, within Dshi_2579 at 2744.985 kb on - strand, within Dshi_2579 at 2745.115 kb on + strand at 2745.123 kb on - strand at 2745.123 kb on - strand at 2745.177 kb on + strand at 2745.308 kb on - strand at 2745.335 kb on - strand at 2745.384 kb on + strand at 2745.414 kb on - strand at 2745.515 kb on + strand at 2745.605 kb on - strand, within Dshi_2580 at 2745.712 kb on + strand, within Dshi_2580 at 2745.746 kb on + strand, within Dshi_2580 at 2745.810 kb on - strand, within Dshi_2580 at 2745.810 kb on - strand, within Dshi_2580 at 2745.810 kb on - strand, within Dshi_2580 at 2745.810 kb on - strand, within Dshi_2580 at 2745.810 kb on - strand, within Dshi_2580 at 2745.823 kb on + strand, within Dshi_2580 at 2746.071 kb on - strand, within Dshi_2580 at 2746.075 kb on - strand, within Dshi_2580
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. L-Lysine 200 mM remove 2,743,741 + +3.0 2,743,855 + -0.4 2,744,681 - +0.8 2,744,810 + Dshi_2579 0.19 +0.2 2,744,825 + Dshi_2579 0.23 +0.4 2,744,833 - Dshi_2579 0.25 -1.7 2,744,833 - Dshi_2579 0.25 +0.8 2,744,833 - Dshi_2579 0.25 -0.6 2,744,942 - Dshi_2579 0.52 -0.9 2,744,942 - Dshi_2579 0.52 +0.4 2,744,945 + Dshi_2579 0.52 +0.2 2,744,985 - Dshi_2579 0.62 -2.0 2,745,115 + +3.8 2,745,123 - -0.6 2,745,123 - -0.9 2,745,177 + -0.9 2,745,308 - -2.0 2,745,335 - -1.1 2,745,384 + -0.2 2,745,414 - +0.3 2,745,515 + +0.4 2,745,605 - Dshi_2580 0.12 -0.2 2,745,712 + Dshi_2580 0.16 +4.4 2,745,746 + Dshi_2580 0.18 +1.4 2,745,810 - Dshi_2580 0.20 -0.4 2,745,810 - Dshi_2580 0.20 +1.2 2,745,810 - Dshi_2580 0.20 +0.3 2,745,810 - Dshi_2580 0.20 +1.2 2,745,810 - Dshi_2580 0.20 +0.2 2,745,823 + Dshi_2580 0.21 +0.0 2,746,071 - Dshi_2580 0.31 -0.2 2,746,075 - Dshi_2580 0.31 +2.8
Or see this region's nucleotide sequence