Experiment: m.b. Cholin acetate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_4198 and Dshi_4199 are separated by 65 nucleotides Dshi_4199 and Dshi_4200 are separated by 145 nucleotides Dshi_4200 and Dshi_4201 overlap by 4 nucleotides
Dshi_4198: Dshi_4198 - transcriptional regulator, LysR family (RefSeq), at 44,976 to 45,887
_4198
Dshi_4199: Dshi_4199 - transcriptional regulator, TetR family (RefSeq), at 45,953 to 46,597
_4199
Dshi_4200: Dshi_4200 - Carbon-monoxide dehydrogenase (acceptor) (RefSeq), at 46,743 to 47,576
_4200
Dshi_4201: Dshi_4201 - (2Fe-2S)-binding domain protein (RefSeq), at 47,573 to 48,073
_4201
Position (kb) 45
46
47 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 45.146 kb on - strand, within Dshi_4198 at 45.189 kb on + strand, within Dshi_4198 at 45.225 kb on + strand, within Dshi_4198 at 45.278 kb on - strand, within Dshi_4198 at 45.470 kb on - strand, within Dshi_4198 at 45.820 kb on + strand at 45.882 kb on + strand at 46.188 kb on - strand, within Dshi_4199 at 46.299 kb on - strand, within Dshi_4199 at 46.322 kb on + strand, within Dshi_4199 at 46.771 kb on + strand at 46.982 kb on + strand, within Dshi_4200 at 47.297 kb on + strand, within Dshi_4200 at 47.316 kb on - strand, within Dshi_4200 at 47.331 kb on + strand, within Dshi_4200 at 47.589 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 40 mM remove 45,146 - Dshi_4198 0.19 +1.7 45,189 + Dshi_4198 0.23 +1.3 45,225 + Dshi_4198 0.27 -2.1 45,278 - Dshi_4198 0.33 -2.3 45,470 - Dshi_4198 0.54 -0.6 45,820 + +2.5 45,882 + -2.8 46,188 - Dshi_4199 0.36 -1.7 46,299 - Dshi_4199 0.54 -2.5 46,322 + Dshi_4199 0.57 +1.5 46,771 + -0.4 46,982 + Dshi_4200 0.29 -0.6 47,297 + Dshi_4200 0.66 +0.6 47,316 - Dshi_4200 0.69 -2.6 47,331 + Dshi_4200 0.71 -1.1 47,589 - +0.2
Or see this region's nucleotide sequence