Experiment: m.b. Cholin acetate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3464 and Dshi_3465 are separated by 12 nucleotides Dshi_3465 and Dshi_3466 are separated by 33 nucleotides
Dshi_3464: Dshi_3464 - heat-inducible transcription repressor HrcA (RefSeq), at 3,644,431 to 3,645,495
_3464
Dshi_3465: Dshi_3465 - GrpE protein (RefSeq), at 3,645,508 to 3,646,101
_3465
Dshi_3466: Dshi_3466 - DNA mismatch repair protein MutS (RefSeq), at 3,646,135 to 3,648,768
_3466
Position (kb)
3645
3646
3647 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3644.535 kb on - strand at 3644.539 kb on + strand, within Dshi_3464 at 3644.550 kb on + strand, within Dshi_3464 at 3644.558 kb on - strand, within Dshi_3464 at 3644.583 kb on - strand, within Dshi_3464 at 3644.653 kb on - strand, within Dshi_3464 at 3644.709 kb on + strand, within Dshi_3464 at 3644.717 kb on - strand, within Dshi_3464 at 3644.720 kb on + strand, within Dshi_3464 at 3644.772 kb on + strand, within Dshi_3464 at 3644.781 kb on + strand, within Dshi_3464 at 3644.786 kb on + strand, within Dshi_3464 at 3644.791 kb on + strand, within Dshi_3464 at 3644.794 kb on - strand, within Dshi_3464 at 3644.796 kb on + strand, within Dshi_3464 at 3644.819 kb on + strand, within Dshi_3464 at 3644.819 kb on + strand, within Dshi_3464 at 3644.819 kb on + strand, within Dshi_3464 at 3644.819 kb on + strand, within Dshi_3464 at 3644.827 kb on - strand, within Dshi_3464 at 3644.838 kb on + strand, within Dshi_3464 at 3644.856 kb on - strand, within Dshi_3464 at 3644.876 kb on + strand, within Dshi_3464 at 3644.884 kb on - strand, within Dshi_3464 at 3644.884 kb on - strand, within Dshi_3464 at 3644.889 kb on - strand, within Dshi_3464 at 3644.892 kb on + strand, within Dshi_3464 at 3644.892 kb on + strand, within Dshi_3464 at 3644.892 kb on + strand, within Dshi_3464 at 3644.893 kb on + strand, within Dshi_3464 at 3644.900 kb on - strand, within Dshi_3464 at 3644.900 kb on - strand, within Dshi_3464 at 3644.900 kb on - strand, within Dshi_3464 at 3644.900 kb on - strand, within Dshi_3464 at 3644.934 kb on - strand, within Dshi_3464 at 3644.956 kb on + strand, within Dshi_3464 at 3645.002 kb on - strand, within Dshi_3464 at 3645.010 kb on - strand, within Dshi_3464 at 3645.014 kb on + strand, within Dshi_3464 at 3645.058 kb on + strand, within Dshi_3464 at 3645.064 kb on + strand, within Dshi_3464 at 3645.100 kb on + strand, within Dshi_3464 at 3645.107 kb on - strand, within Dshi_3464 at 3645.203 kb on + strand, within Dshi_3464 at 3645.204 kb on + strand, within Dshi_3464 at 3645.267 kb on - strand, within Dshi_3464 at 3645.271 kb on - strand, within Dshi_3464 at 3645.271 kb on - strand, within Dshi_3464 at 3645.273 kb on + strand, within Dshi_3464 at 3645.273 kb on + strand, within Dshi_3464 at 3645.281 kb on - strand, within Dshi_3464 at 3645.282 kb on + strand, within Dshi_3464 at 3645.292 kb on - strand, within Dshi_3464 at 3645.292 kb on - strand, within Dshi_3464 at 3645.373 kb on + strand, within Dshi_3464 at 3645.415 kb on + strand at 3645.474 kb on + strand at 3645.474 kb on + strand at 3645.474 kb on + strand at 3646.085 kb on - strand at 3646.127 kb on - strand at 3646.369 kb on + strand at 3646.415 kb on + strand, within Dshi_3466 at 3646.508 kb on + strand, within Dshi_3466 at 3646.529 kb on - strand, within Dshi_3466 at 3646.620 kb on + strand, within Dshi_3466 at 3646.672 kb on + strand, within Dshi_3466 at 3646.882 kb on + strand, within Dshi_3466 at 3646.908 kb on + strand, within Dshi_3466 at 3646.994 kb on - strand, within Dshi_3466 at 3647.056 kb on + strand, within Dshi_3466
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 40 mM remove 3,644,535 - +1.2 3,644,539 + Dshi_3464 0.10 -0.9 3,644,550 + Dshi_3464 0.11 +0.3 3,644,558 - Dshi_3464 0.12 -1.5 3,644,583 - Dshi_3464 0.14 -0.2 3,644,653 - Dshi_3464 0.21 +0.8 3,644,709 + Dshi_3464 0.26 -3.4 3,644,717 - Dshi_3464 0.27 -0.6 3,644,720 + Dshi_3464 0.27 +1.7 3,644,772 + Dshi_3464 0.32 -1.9 3,644,781 + Dshi_3464 0.33 -0.4 3,644,786 + Dshi_3464 0.33 -0.4 3,644,791 + Dshi_3464 0.34 +0.2 3,644,794 - Dshi_3464 0.34 -0.1 3,644,796 + Dshi_3464 0.34 -0.0 3,644,819 + Dshi_3464 0.36 +0.1 3,644,819 + Dshi_3464 0.36 -0.1 3,644,819 + Dshi_3464 0.36 -0.6 3,644,819 + Dshi_3464 0.36 -0.6 3,644,827 - Dshi_3464 0.37 -0.8 3,644,838 + Dshi_3464 0.38 -0.4 3,644,856 - Dshi_3464 0.40 -0.8 3,644,876 + Dshi_3464 0.42 -0.3 3,644,884 - Dshi_3464 0.43 -1.2 3,644,884 - Dshi_3464 0.43 -1.2 3,644,889 - Dshi_3464 0.43 -0.8 3,644,892 + Dshi_3464 0.43 +0.1 3,644,892 + Dshi_3464 0.43 +0.8 3,644,892 + Dshi_3464 0.43 -0.7 3,644,893 + Dshi_3464 0.43 -2.6 3,644,900 - Dshi_3464 0.44 +0.6 3,644,900 - Dshi_3464 0.44 -0.0 3,644,900 - Dshi_3464 0.44 -0.7 3,644,900 - Dshi_3464 0.44 -1.5 3,644,934 - Dshi_3464 0.47 -0.3 3,644,956 + Dshi_3464 0.49 -0.9 3,645,002 - Dshi_3464 0.54 -1.2 3,645,010 - Dshi_3464 0.54 -0.4 3,645,014 + Dshi_3464 0.55 -0.6 3,645,058 + Dshi_3464 0.59 -0.6 3,645,064 + Dshi_3464 0.59 -0.6 3,645,100 + Dshi_3464 0.63 -0.4 3,645,107 - Dshi_3464 0.63 -2.6 3,645,203 + Dshi_3464 0.72 -3.6 3,645,204 + Dshi_3464 0.73 -3.0 3,645,267 - Dshi_3464 0.78 -0.7 3,645,271 - Dshi_3464 0.79 -0.8 3,645,271 - Dshi_3464 0.79 -0.5 3,645,273 + Dshi_3464 0.79 -1.6 3,645,273 + Dshi_3464 0.79 -0.1 3,645,281 - Dshi_3464 0.80 -0.4 3,645,282 + Dshi_3464 0.80 +0.7 3,645,292 - Dshi_3464 0.81 +0.3 3,645,292 - Dshi_3464 0.81 -1.1 3,645,373 + Dshi_3464 0.88 -3.8 3,645,415 + +1.4 3,645,474 + -1.6 3,645,474 + -2.7 3,645,474 + -1.3 3,646,085 - -2.6 3,646,127 - -1.8 3,646,369 + -0.9 3,646,415 + Dshi_3466 0.11 -0.2 3,646,508 + Dshi_3466 0.14 -0.4 3,646,529 - Dshi_3466 0.15 -0.8 3,646,620 + Dshi_3466 0.18 -0.1 3,646,672 + Dshi_3466 0.20 +0.9 3,646,882 + Dshi_3466 0.28 +0.5 3,646,908 + Dshi_3466 0.29 +1.5 3,646,994 - Dshi_3466 0.33 +0.7 3,647,056 + Dshi_3466 0.35 -3.7
Or see this region's nucleotide sequence