Experiment: m.b. Cholin acetate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3444 and Dshi_3445 overlap by 4 nucleotides Dshi_3445 and Dshi_3446 are separated by 106 nucleotides Dshi_3446 and Dshi_3447 are separated by 57 nucleotides Dshi_3447 and Dshi_3448 are separated by 4 nucleotides
Dshi_3444: Dshi_3444 - MltA domain protein (RefSeq), at 3,627,798 to 3,628,826
_3444
Dshi_3445: Dshi_3445 - import inner membrane translocase subunit Tim44 (RefSeq), at 3,628,823 to 3,629,488
_3445
Dshi_3446: Dshi_3446 - FxsA cytoplasmic membrane protein (RefSeq), at 3,629,595 to 3,630,086
_3446
Dshi_3447: Dshi_3447 - protein-export protein SecB (RefSeq), at 3,630,144 to 3,630,662
_3447
Dshi_3448: Dshi_3448 - DNA polymerase III, epsilon subunit (RefSeq), at 3,630,667 to 3,631,359
_3448
Position (kb)
3629
3630
3631 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3628.653 kb on + strand, within Dshi_3444 at 3628.657 kb on + strand, within Dshi_3444 at 3628.662 kb on + strand, within Dshi_3444 at 3628.679 kb on - strand, within Dshi_3444 at 3628.711 kb on - strand, within Dshi_3444 at 3628.734 kb on - strand at 3628.793 kb on - strand at 3628.797 kb on + strand at 3628.890 kb on + strand, within Dshi_3445 at 3628.954 kb on + strand, within Dshi_3445 at 3629.037 kb on + strand, within Dshi_3445 at 3629.070 kb on + strand, within Dshi_3445 at 3629.078 kb on - strand, within Dshi_3445 at 3629.246 kb on + strand, within Dshi_3445 at 3629.279 kb on + strand, within Dshi_3445 at 3629.279 kb on + strand, within Dshi_3445 at 3629.327 kb on - strand, within Dshi_3445 at 3629.448 kb on + strand at 3629.448 kb on + strand at 3629.448 kb on + strand at 3629.448 kb on + strand at 3629.456 kb on - strand at 3629.493 kb on + strand at 3629.538 kb on - strand at 3629.544 kb on + strand at 3629.545 kb on + strand at 3629.545 kb on + strand at 3629.547 kb on + strand at 3629.553 kb on - strand at 3629.586 kb on - strand at 3629.612 kb on + strand at 3629.612 kb on + strand at 3629.620 kb on - strand at 3629.620 kb on - strand at 3629.620 kb on - strand at 3629.638 kb on - strand at 3629.643 kb on + strand at 3629.645 kb on + strand, within Dshi_3446 at 3629.645 kb on + strand, within Dshi_3446 at 3629.654 kb on + strand, within Dshi_3446 at 3629.654 kb on + strand, within Dshi_3446 at 3629.662 kb on - strand, within Dshi_3446 at 3629.662 kb on - strand, within Dshi_3446 at 3629.662 kb on - strand, within Dshi_3446 at 3629.665 kb on + strand, within Dshi_3446 at 3629.665 kb on + strand, within Dshi_3446 at 3629.665 kb on + strand, within Dshi_3446 at 3629.673 kb on - strand, within Dshi_3446 at 3629.673 kb on - strand, within Dshi_3446 at 3629.677 kb on + strand, within Dshi_3446 at 3629.677 kb on + strand, within Dshi_3446 at 3629.685 kb on - strand, within Dshi_3446 at 3629.685 kb on - strand, within Dshi_3446 at 3629.711 kb on + strand, within Dshi_3446 at 3629.711 kb on + strand, within Dshi_3446 at 3629.711 kb on + strand, within Dshi_3446 at 3629.719 kb on - strand, within Dshi_3446 at 3629.719 kb on - strand, within Dshi_3446 at 3629.719 kb on - strand, within Dshi_3446 at 3629.719 kb on - strand, within Dshi_3446 at 3629.729 kb on - strand, within Dshi_3446 at 3629.729 kb on - strand, within Dshi_3446 at 3629.729 kb on - strand, within Dshi_3446 at 3629.748 kb on - strand, within Dshi_3446 at 3629.771 kb on - strand, within Dshi_3446 at 3629.799 kb on + strand, within Dshi_3446 at 3629.808 kb on - strand, within Dshi_3446 at 3629.833 kb on - strand, within Dshi_3446 at 3629.838 kb on + strand, within Dshi_3446 at 3629.838 kb on + strand, within Dshi_3446 at 3629.838 kb on + strand, within Dshi_3446 at 3629.846 kb on - strand, within Dshi_3446 at 3629.868 kb on + strand, within Dshi_3446 at 3629.920 kb on - strand, within Dshi_3446 at 3629.933 kb on + strand, within Dshi_3446 at 3629.975 kb on - strand, within Dshi_3446 at 3629.983 kb on + strand, within Dshi_3446 at 3629.991 kb on - strand, within Dshi_3446 at 3630.033 kb on + strand, within Dshi_3446 at 3630.038 kb on - strand at 3630.049 kb on + strand at 3630.088 kb on - strand at 3630.273 kb on + strand, within Dshi_3447 at 3630.273 kb on + strand, within Dshi_3447 at 3630.273 kb on + strand, within Dshi_3447 at 3630.274 kb on - strand, within Dshi_3447 at 3630.277 kb on - strand, within Dshi_3447 at 3630.281 kb on - strand, within Dshi_3447 at 3630.301 kb on + strand, within Dshi_3447 at 3630.325 kb on - strand, within Dshi_3447 at 3630.415 kb on + strand, within Dshi_3447 at 3630.470 kb on + strand, within Dshi_3447 at 3630.478 kb on - strand, within Dshi_3447 at 3630.496 kb on - strand, within Dshi_3447 at 3630.579 kb on - strand, within Dshi_3447 at 3630.628 kb on - strand at 3630.656 kb on + strand at 3630.817 kb on + strand, within Dshi_3448
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 40 mM remove 3,628,653 + Dshi_3444 0.83 +0.2 3,628,657 + Dshi_3444 0.83 -0.3 3,628,662 + Dshi_3444 0.84 -2.0 3,628,679 - Dshi_3444 0.86 -0.3 3,628,711 - Dshi_3444 0.89 +0.3 3,628,734 - +0.0 3,628,793 - +0.5 3,628,797 + +0.2 3,628,890 + Dshi_3445 0.10 +1.6 3,628,954 + Dshi_3445 0.20 +1.0 3,629,037 + Dshi_3445 0.32 +2.1 3,629,070 + Dshi_3445 0.37 +1.0 3,629,078 - Dshi_3445 0.38 +1.4 3,629,246 + Dshi_3445 0.64 +1.9 3,629,279 + Dshi_3445 0.68 -0.0 3,629,279 + Dshi_3445 0.68 +0.2 3,629,327 - Dshi_3445 0.76 +1.5 3,629,448 + +1.5 3,629,448 + +1.2 3,629,448 + +1.0 3,629,448 + +0.9 3,629,456 - +2.0 3,629,493 + +0.7 3,629,538 - +1.0 3,629,544 + +0.5 3,629,545 + +0.4 3,629,545 + +1.8 3,629,547 + +2.2 3,629,553 - +0.8 3,629,586 - -1.5 3,629,612 + +1.0 3,629,612 + +0.7 3,629,620 - -0.6 3,629,620 - +0.2 3,629,620 - +0.0 3,629,638 - -0.1 3,629,643 + -0.7 3,629,645 + Dshi_3446 0.10 -0.3 3,629,645 + Dshi_3446 0.10 -0.2 3,629,654 + Dshi_3446 0.12 -0.3 3,629,654 + Dshi_3446 0.12 -0.4 3,629,662 - Dshi_3446 0.14 -0.9 3,629,662 - Dshi_3446 0.14 -0.9 3,629,662 - Dshi_3446 0.14 -0.3 3,629,665 + Dshi_3446 0.14 +0.7 3,629,665 + Dshi_3446 0.14 -2.8 3,629,665 + Dshi_3446 0.14 +1.0 3,629,673 - Dshi_3446 0.16 +0.7 3,629,673 - Dshi_3446 0.16 -2.6 3,629,677 + Dshi_3446 0.17 +0.3 3,629,677 + Dshi_3446 0.17 -3.0 3,629,685 - Dshi_3446 0.18 -0.8 3,629,685 - Dshi_3446 0.18 -1.7 3,629,711 + Dshi_3446 0.24 -0.2 3,629,711 + Dshi_3446 0.24 -1.1 3,629,711 + Dshi_3446 0.24 -1.2 3,629,719 - Dshi_3446 0.25 +0.2 3,629,719 - Dshi_3446 0.25 +0.4 3,629,719 - Dshi_3446 0.25 -0.2 3,629,719 - Dshi_3446 0.25 -0.1 3,629,729 - Dshi_3446 0.27 -0.9 3,629,729 - Dshi_3446 0.27 -0.2 3,629,729 - Dshi_3446 0.27 +0.4 3,629,748 - Dshi_3446 0.31 -0.6 3,629,771 - Dshi_3446 0.36 +0.7 3,629,799 + Dshi_3446 0.41 -1.1 3,629,808 - Dshi_3446 0.43 -0.1 3,629,833 - Dshi_3446 0.48 -0.0 3,629,838 + Dshi_3446 0.49 -0.8 3,629,838 + Dshi_3446 0.49 -0.6 3,629,838 + Dshi_3446 0.49 -0.1 3,629,846 - Dshi_3446 0.51 -0.1 3,629,868 + Dshi_3446 0.55 -1.6 3,629,920 - Dshi_3446 0.66 -0.4 3,629,933 + Dshi_3446 0.69 +0.1 3,629,975 - Dshi_3446 0.77 -1.7 3,629,983 + Dshi_3446 0.79 -0.3 3,629,991 - Dshi_3446 0.80 -1.3 3,630,033 + Dshi_3446 0.89 -0.5 3,630,038 - -2.4 3,630,049 + -1.8 3,630,088 - +0.6 3,630,273 + Dshi_3447 0.25 -0.1 3,630,273 + Dshi_3447 0.25 -0.4 3,630,273 + Dshi_3447 0.25 -0.7 3,630,274 - Dshi_3447 0.25 -1.2 3,630,277 - Dshi_3447 0.26 -3.0 3,630,281 - Dshi_3447 0.26 -0.6 3,630,301 + Dshi_3447 0.30 -2.9 3,630,325 - Dshi_3447 0.35 -3.7 3,630,415 + Dshi_3447 0.52 -2.6 3,630,470 + Dshi_3447 0.63 -1.1 3,630,478 - Dshi_3447 0.64 -1.6 3,630,496 - Dshi_3447 0.68 -1.2 3,630,579 - Dshi_3447 0.84 -1.7 3,630,628 - -0.2 3,630,656 + -0.9 3,630,817 + Dshi_3448 0.22 -4.4
Or see this region's nucleotide sequence