Experiment: m.b. Cholin acetate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3309 and Dshi_3310 are separated by 53 nucleotides Dshi_3310 and Dshi_3311 overlap by 1 nucleotides Dshi_3311 and Dshi_3312 are separated by 146 nucleotides Dshi_3312 and Dshi_3313 overlap by 4 nucleotides
Dshi_3309: Dshi_3309 - monofunctional biosynthetic peptidoglycan transglycosylase (RefSeq), at 3,472,552 to 3,473,298
_3309
Dshi_3310: Dshi_3310 - Patatin (RefSeq), at 3,473,352 to 3,474,155
_3310
Dshi_3311: Dshi_3311 - radical SAM enzyme, Cfr family (RefSeq), at 3,474,155 to 3,475,375
_3311
Dshi_3312: Dshi_3312 - hypothetical protein (RefSeq), at 3,475,522 to 3,476,199
_3312
Dshi_3313: Dshi_3313 - hypothetical protein (RefSeq), at 3,476,196 to 3,476,906
_3313
Position (kb)
3474
3475
3476 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3473.266 kb on - strand at 3473.282 kb on + strand at 3473.284 kb on + strand at 3473.312 kb on + strand at 3473.325 kb on - strand at 3473.340 kb on + strand at 3473.468 kb on - strand, within Dshi_3310 at 3473.550 kb on + strand, within Dshi_3310 at 3473.567 kb on - strand, within Dshi_3310 at 3473.593 kb on - strand, within Dshi_3310 at 3473.617 kb on + strand, within Dshi_3310 at 3473.808 kb on + strand, within Dshi_3310 at 3473.816 kb on - strand, within Dshi_3310 at 3473.916 kb on - strand, within Dshi_3310 at 3474.063 kb on - strand, within Dshi_3310 at 3474.283 kb on - strand, within Dshi_3311 at 3474.444 kb on + strand, within Dshi_3311 at 3474.452 kb on - strand, within Dshi_3311 at 3474.490 kb on + strand, within Dshi_3311 at 3474.500 kb on + strand, within Dshi_3311 at 3474.500 kb on + strand, within Dshi_3311 at 3474.526 kb on - strand, within Dshi_3311 at 3474.576 kb on - strand, within Dshi_3311 at 3474.576 kb on - strand, within Dshi_3311 at 3474.601 kb on + strand, within Dshi_3311 at 3474.654 kb on + strand, within Dshi_3311 at 3474.677 kb on + strand, within Dshi_3311 at 3474.727 kb on - strand, within Dshi_3311 at 3474.753 kb on + strand, within Dshi_3311 at 3474.817 kb on - strand, within Dshi_3311 at 3474.846 kb on + strand, within Dshi_3311 at 3474.874 kb on + strand, within Dshi_3311 at 3474.963 kb on + strand, within Dshi_3311 at 3474.997 kb on + strand, within Dshi_3311 at 3475.033 kb on - strand, within Dshi_3311 at 3475.113 kb on + strand, within Dshi_3311 at 3475.115 kb on - strand, within Dshi_3311 at 3475.143 kb on - strand, within Dshi_3311 at 3475.245 kb on + strand, within Dshi_3311 at 3475.250 kb on + strand, within Dshi_3311 at 3475.250 kb on + strand at 3475.258 kb on - strand at 3475.258 kb on - strand at 3475.282 kb on - strand at 3475.290 kb on - strand at 3475.301 kb on + strand at 3475.305 kb on + strand at 3475.345 kb on - strand at 3475.349 kb on + strand at 3475.373 kb on - strand at 3475.373 kb on - strand at 3475.494 kb on - strand at 3475.496 kb on + strand at 3475.524 kb on + strand at 3475.524 kb on + strand at 3475.532 kb on - strand at 3475.532 kb on - strand at 3475.537 kb on + strand at 3475.545 kb on - strand at 3475.545 kb on - strand at 3475.622 kb on - strand, within Dshi_3312 at 3475.652 kb on + strand, within Dshi_3312 at 3475.660 kb on - strand, within Dshi_3312 at 3475.699 kb on - strand, within Dshi_3312 at 3475.699 kb on - strand, within Dshi_3312 at 3475.704 kb on + strand, within Dshi_3312 at 3475.734 kb on - strand, within Dshi_3312 at 3475.783 kb on - strand, within Dshi_3312 at 3475.838 kb on + strand, within Dshi_3312 at 3476.194 kb on + strand at 3476.245 kb on + strand at 3476.269 kb on - strand, within Dshi_3313 at 3476.277 kb on + strand, within Dshi_3313 at 3476.285 kb on - strand, within Dshi_3313 at 3476.319 kb on - strand, within Dshi_3313 at 3476.338 kb on - strand, within Dshi_3313
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 40 mM remove 3,473,266 - +1.7 3,473,282 + +0.6 3,473,284 + +1.3 3,473,312 + -0.8 3,473,325 - -2.4 3,473,340 + +0.5 3,473,468 - Dshi_3310 0.14 +0.6 3,473,550 + Dshi_3310 0.25 +1.2 3,473,567 - Dshi_3310 0.27 +2.2 3,473,593 - Dshi_3310 0.30 -1.0 3,473,617 + Dshi_3310 0.33 -0.0 3,473,808 + Dshi_3310 0.57 +1.1 3,473,816 - Dshi_3310 0.58 -0.8 3,473,916 - Dshi_3310 0.70 -1.0 3,474,063 - Dshi_3310 0.88 -0.6 3,474,283 - Dshi_3311 0.10 -1.6 3,474,444 + Dshi_3311 0.24 +1.7 3,474,452 - Dshi_3311 0.24 -1.0 3,474,490 + Dshi_3311 0.27 +0.8 3,474,500 + Dshi_3311 0.28 -0.7 3,474,500 + Dshi_3311 0.28 +1.4 3,474,526 - Dshi_3311 0.30 -0.6 3,474,576 - Dshi_3311 0.34 +1.4 3,474,576 - Dshi_3311 0.34 +0.1 3,474,601 + Dshi_3311 0.37 -0.6 3,474,654 + Dshi_3311 0.41 -1.6 3,474,677 + Dshi_3311 0.43 -1.2 3,474,727 - Dshi_3311 0.47 -0.9 3,474,753 + Dshi_3311 0.49 -1.4 3,474,817 - Dshi_3311 0.54 -3.0 3,474,846 + Dshi_3311 0.57 +0.1 3,474,874 + Dshi_3311 0.59 -0.5 3,474,963 + Dshi_3311 0.66 -0.8 3,474,997 + Dshi_3311 0.69 -1.8 3,475,033 - Dshi_3311 0.72 +1.0 3,475,113 + Dshi_3311 0.78 -1.0 3,475,115 - Dshi_3311 0.79 -0.8 3,475,143 - Dshi_3311 0.81 +1.4 3,475,245 + Dshi_3311 0.89 +0.5 3,475,250 + Dshi_3311 0.90 -1.9 3,475,250 + +0.8 3,475,258 - -2.2 3,475,258 - -1.8 3,475,282 - +0.1 3,475,290 - -1.4 3,475,301 + +0.7 3,475,305 + +1.4 3,475,345 - +0.2 3,475,349 + +0.9 3,475,373 - +0.5 3,475,373 - +3.4 3,475,494 - -0.9 3,475,496 + -1.0 3,475,524 + +0.8 3,475,524 + -2.2 3,475,532 - -2.0 3,475,532 - -0.6 3,475,537 + -1.3 3,475,545 - -0.6 3,475,545 - +0.1 3,475,622 - Dshi_3312 0.15 +0.7 3,475,652 + Dshi_3312 0.19 +1.2 3,475,660 - Dshi_3312 0.20 +0.3 3,475,699 - Dshi_3312 0.26 +4.4 3,475,699 - Dshi_3312 0.26 +3.0 3,475,704 + Dshi_3312 0.27 -0.8 3,475,734 - Dshi_3312 0.31 +0.3 3,475,783 - Dshi_3312 0.38 -0.2 3,475,838 + Dshi_3312 0.47 +2.4 3,476,194 + -0.7 3,476,245 + -1.1 3,476,269 - Dshi_3313 0.10 +4.0 3,476,277 + Dshi_3313 0.11 +2.3 3,476,285 - Dshi_3313 0.13 +2.4 3,476,319 - Dshi_3313 0.17 -2.3 3,476,338 - Dshi_3313 0.20 +0.9
Or see this region's nucleotide sequence