Experiment: m.b. Cholin acetate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3207 and Dshi_3208 are separated by 275 nucleotides Dshi_3208 and Dshi_3209 overlap by 4 nucleotides
Dshi_3207: Dshi_3207 - protein of unknown function DUF989 (RefSeq), at 3,371,394 to 3,372,626
_3207
Dshi_3208: Dshi_3208 - hypothetical protein (RefSeq), at 3,372,902 to 3,374,383
_3208
Dshi_3209: Dshi_3209 - hypothetical protein (RefSeq), at 3,374,380 to 3,375,372
_3209
Position (kb)
3372
3373
3374
3375 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3371.908 kb on - strand, within Dshi_3207 at 3372.011 kb on + strand, within Dshi_3207 at 3372.019 kb on - strand, within Dshi_3207 at 3372.019 kb on - strand, within Dshi_3207 at 3372.067 kb on - strand, within Dshi_3207 at 3372.080 kb on - strand, within Dshi_3207 at 3372.144 kb on + strand, within Dshi_3207 at 3372.292 kb on - strand, within Dshi_3207 at 3372.404 kb on - strand, within Dshi_3207 at 3372.620 kb on + strand at 3372.690 kb on + strand at 3372.690 kb on - strand at 3372.699 kb on - strand at 3372.710 kb on + strand at 3372.710 kb on + strand at 3372.710 kb on + strand at 3372.718 kb on - strand at 3372.718 kb on - strand at 3372.718 kb on - strand at 3372.718 kb on - strand at 3372.718 kb on - strand at 3372.728 kb on - strand at 3372.775 kb on - strand at 3372.893 kb on + strand at 3373.043 kb on + strand at 3373.120 kb on - strand, within Dshi_3208 at 3373.161 kb on - strand, within Dshi_3208 at 3373.169 kb on + strand, within Dshi_3208 at 3373.169 kb on + strand, within Dshi_3208 at 3373.187 kb on + strand, within Dshi_3208 at 3373.232 kb on + strand, within Dshi_3208 at 3373.298 kb on + strand, within Dshi_3208 at 3373.320 kb on - strand, within Dshi_3208 at 3373.341 kb on + strand, within Dshi_3208 at 3373.375 kb on - strand, within Dshi_3208 at 3373.434 kb on + strand, within Dshi_3208 at 3373.447 kb on + strand, within Dshi_3208 at 3373.467 kb on + strand, within Dshi_3208 at 3373.494 kb on - strand, within Dshi_3208 at 3373.748 kb on + strand, within Dshi_3208 at 3373.750 kb on - strand, within Dshi_3208 at 3373.812 kb on + strand, within Dshi_3208 at 3373.820 kb on - strand, within Dshi_3208 at 3373.820 kb on - strand, within Dshi_3208 at 3373.895 kb on + strand, within Dshi_3208 at 3374.026 kb on + strand, within Dshi_3208 at 3374.048 kb on - strand, within Dshi_3208 at 3374.302 kb on + strand at 3374.369 kb on - strand at 3374.385 kb on + strand at 3374.387 kb on - strand at 3374.414 kb on - strand at 3374.441 kb on + strand at 3374.474 kb on + strand at 3374.617 kb on - strand, within Dshi_3209 at 3374.697 kb on + strand, within Dshi_3209 at 3374.726 kb on + strand, within Dshi_3209 at 3374.794 kb on + strand, within Dshi_3209 at 3374.804 kb on + strand, within Dshi_3209 at 3374.860 kb on - strand, within Dshi_3209 at 3374.869 kb on + strand, within Dshi_3209 at 3374.888 kb on - strand, within Dshi_3209 at 3374.888 kb on - strand, within Dshi_3209 at 3374.888 kb on - strand, within Dshi_3209 at 3374.895 kb on - strand, within Dshi_3209 at 3375.028 kb on - strand, within Dshi_3209 at 3375.076 kb on - strand, within Dshi_3209 at 3375.099 kb on - strand, within Dshi_3209 at 3375.100 kb on + strand, within Dshi_3209 at 3375.106 kb on + strand, within Dshi_3209 at 3375.126 kb on + strand, within Dshi_3209 at 3375.189 kb on - strand, within Dshi_3209 at 3375.225 kb on + strand, within Dshi_3209 at 3375.228 kb on - strand, within Dshi_3209 at 3375.233 kb on - strand, within Dshi_3209 at 3375.308 kb on + strand at 3375.359 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 40 mM remove 3,371,908 - Dshi_3207 0.42 -2.6 3,372,011 + Dshi_3207 0.50 -1.2 3,372,019 - Dshi_3207 0.51 -2.6 3,372,019 - Dshi_3207 0.51 -2.7 3,372,067 - Dshi_3207 0.55 +1.1 3,372,080 - Dshi_3207 0.56 -3.0 3,372,144 + Dshi_3207 0.61 +1.5 3,372,292 - Dshi_3207 0.73 -0.6 3,372,404 - Dshi_3207 0.82 -1.4 3,372,620 + +0.6 3,372,690 + -3.3 3,372,690 - -0.2 3,372,699 - -0.8 3,372,710 + +1.2 3,372,710 + +2.4 3,372,710 + -0.8 3,372,718 - -0.9 3,372,718 - +1.6 3,372,718 - +0.2 3,372,718 - +2.4 3,372,718 - -0.2 3,372,728 - +2.4 3,372,775 - +0.1 3,372,893 + +2.4 3,373,043 + +0.1 3,373,120 - Dshi_3208 0.15 +1.2 3,373,161 - Dshi_3208 0.17 +3.4 3,373,169 + Dshi_3208 0.18 -0.0 3,373,169 + Dshi_3208 0.18 -0.1 3,373,187 + Dshi_3208 0.19 +0.4 3,373,232 + Dshi_3208 0.22 +0.3 3,373,298 + Dshi_3208 0.27 +0.8 3,373,320 - Dshi_3208 0.28 -2.8 3,373,341 + Dshi_3208 0.30 -2.3 3,373,375 - Dshi_3208 0.32 -0.3 3,373,434 + Dshi_3208 0.36 +0.1 3,373,447 + Dshi_3208 0.37 -1.4 3,373,467 + Dshi_3208 0.38 +1.0 3,373,494 - Dshi_3208 0.40 +0.8 3,373,748 + Dshi_3208 0.57 -1.0 3,373,750 - Dshi_3208 0.57 -0.2 3,373,812 + Dshi_3208 0.61 -1.8 3,373,820 - Dshi_3208 0.62 +1.4 3,373,820 - Dshi_3208 0.62 +2.4 3,373,895 + Dshi_3208 0.67 +1.4 3,374,026 + Dshi_3208 0.76 -2.4 3,374,048 - Dshi_3208 0.77 +0.5 3,374,302 + -2.4 3,374,369 - -0.3 3,374,385 + +1.1 3,374,387 - -0.4 3,374,414 - -0.9 3,374,441 + +0.6 3,374,474 + -0.6 3,374,617 - Dshi_3209 0.24 +0.1 3,374,697 + Dshi_3209 0.32 -0.2 3,374,726 + Dshi_3209 0.35 +1.4 3,374,794 + Dshi_3209 0.42 +1.2 3,374,804 + Dshi_3209 0.43 -3.0 3,374,860 - Dshi_3209 0.48 +0.5 3,374,869 + Dshi_3209 0.49 +0.8 3,374,888 - Dshi_3209 0.51 -1.2 3,374,888 - Dshi_3209 0.51 -1.3 3,374,888 - Dshi_3209 0.51 +4.1 3,374,895 - Dshi_3209 0.52 -1.3 3,375,028 - Dshi_3209 0.65 +0.8 3,375,076 - Dshi_3209 0.70 -0.8 3,375,099 - Dshi_3209 0.72 -0.8 3,375,100 + Dshi_3209 0.73 +0.5 3,375,106 + Dshi_3209 0.73 -2.0 3,375,126 + Dshi_3209 0.75 +0.3 3,375,189 - Dshi_3209 0.81 +0.5 3,375,225 + Dshi_3209 0.85 +0.2 3,375,228 - Dshi_3209 0.85 +0.4 3,375,233 - Dshi_3209 0.86 -1.0 3,375,308 + -0.2 3,375,359 - -1.1
Or see this region's nucleotide sequence