Experiment: m.b. Cholin acetate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2955 and Dshi_2956 are separated by 88 nucleotides Dshi_2956 and Dshi_2957 are separated by 226 nucleotides Dshi_2957 and Dshi_2958 are separated by 245 nucleotides
Dshi_2955: Dshi_2955 - histidyl-tRNA synthetase (RefSeq), at 3,115,954 to 3,117,447
_2955
Dshi_2956: Dshi_2956 - SlyX family protein (RefSeq), at 3,117,536 to 3,117,745
_2956
Dshi_2957: Dshi_2957 - DNA polymerase III, alpha subunit (RefSeq), at 3,117,972 to 3,121,466
_2957
Dshi_2958: Dshi_2958 - Xanthine dehydrogenase (EC 1.17.1.4) (from data) , at 3,121,712 to 3,123,163
_2958
Position (kb)
3117
3118
3119
3120
3121
3122 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3117.532 kb on + strand at 3117.542 kb on - strand at 3117.542 kb on - strand at 3117.791 kb on - strand at 3117.824 kb on - strand at 3121.553 kb on - strand at 3121.582 kb on - strand at 3121.655 kb on - strand at 3121.656 kb on + strand at 3121.673 kb on + strand at 3121.681 kb on - strand at 3121.813 kb on - strand at 3121.815 kb on + strand at 3121.947 kb on - strand, within Dshi_2958 at 3122.164 kb on - strand, within Dshi_2958 at 3122.184 kb on + strand, within Dshi_2958 at 3122.303 kb on + strand, within Dshi_2958 at 3122.343 kb on + strand, within Dshi_2958 at 3122.404 kb on + strand, within Dshi_2958
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 40 mM remove 3,117,532 + +1.0 3,117,542 - -0.5 3,117,542 - -0.8 3,117,791 - +0.1 3,117,824 - -0.9 3,121,553 - +1.4 3,121,582 - +0.7 3,121,655 - -0.8 3,121,656 + +0.7 3,121,673 + +1.4 3,121,681 - -0.2 3,121,813 - +0.6 3,121,815 + -0.2 3,121,947 - Dshi_2958 0.16 +2.4 3,122,164 - Dshi_2958 0.31 +1.1 3,122,184 + Dshi_2958 0.33 +0.5 3,122,303 + Dshi_2958 0.41 +0.4 3,122,343 + Dshi_2958 0.43 +1.8 3,122,404 + Dshi_2958 0.48 -1.9
Or see this region's nucleotide sequence