Experiment: m.b. Cholin acetate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2810 and Dshi_2811 overlap by 4 nucleotides Dshi_2811 and Dshi_2812 are separated by 21 nucleotides Dshi_2812 and Dshi_2813 are separated by 20 nucleotides Dshi_2813 and Dshi_2814 are separated by 12 nucleotides
Dshi_2810: Dshi_2810 - regulatory protein SoxS (RefSeq), at 2,967,368 to 2,967,775
_2810
Dshi_2811: Dshi_2811 - protein of unknown function DUF336 (RefSeq), at 2,967,772 to 2,968,284
_2811
Dshi_2812: Dshi_2812 - transcriptional regulator, ArsR family (RefSeq), at 2,968,306 to 2,968,641
_2812
Dshi_2813: Dshi_2813 - protein of unknown function DUF395 YeeE/YedE (RefSeq), at 2,968,662 to 2,969,750
_2813
Dshi_2814: Dshi_2814 - diguanylate cyclase (RefSeq), at 2,969,763 to 2,970,803
_2814
Position (kb)
2968
2969
2970 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2967.666 kb on - strand, within Dshi_2810 at 2967.666 kb on - strand, within Dshi_2810 at 2967.750 kb on + strand at 2967.750 kb on + strand at 2967.881 kb on - strand, within Dshi_2811 at 2967.931 kb on + strand, within Dshi_2811 at 2968.007 kb on - strand, within Dshi_2811 at 2968.015 kb on + strand, within Dshi_2811 at 2968.062 kb on - strand, within Dshi_2811 at 2968.224 kb on - strand, within Dshi_2811 at 2968.253 kb on - strand at 2968.310 kb on - strand at 2968.332 kb on + strand at 2968.340 kb on + strand, within Dshi_2812 at 2968.635 kb on + strand at 2968.732 kb on - strand at 2968.754 kb on - strand at 2968.754 kb on - strand at 2968.843 kb on + strand, within Dshi_2813 at 2968.846 kb on - strand, within Dshi_2813 at 2968.848 kb on + strand, within Dshi_2813 at 2968.914 kb on - strand, within Dshi_2813 at 2969.021 kb on - strand, within Dshi_2813 at 2969.030 kb on - strand, within Dshi_2813 at 2969.102 kb on + strand, within Dshi_2813 at 2969.177 kb on + strand, within Dshi_2813 at 2969.363 kb on + strand at 2969.424 kb on - strand, within Dshi_2813 at 2969.439 kb on + strand, within Dshi_2813 at 2969.439 kb on + strand, within Dshi_2813 at 2969.487 kb on + strand, within Dshi_2813 at 2969.487 kb on + strand, within Dshi_2813 at 2969.487 kb on + strand, within Dshi_2813 at 2969.490 kb on - strand, within Dshi_2813 at 2969.495 kb on - strand, within Dshi_2813 at 2969.495 kb on - strand, within Dshi_2813 at 2969.513 kb on + strand, within Dshi_2813 at 2969.521 kb on - strand, within Dshi_2813 at 2969.521 kb on - strand, within Dshi_2813 at 2969.573 kb on + strand, within Dshi_2813 at 2969.603 kb on + strand, within Dshi_2813 at 2969.661 kb on - strand at 2969.784 kb on + strand at 2969.815 kb on + strand at 2969.846 kb on - strand at 2969.846 kb on - strand at 2969.847 kb on + strand at 2969.847 kb on + strand at 2969.964 kb on + strand, within Dshi_2814 at 2970.104 kb on + strand, within Dshi_2814 at 2970.115 kb on + strand, within Dshi_2814 at 2970.123 kb on - strand, within Dshi_2814 at 2970.282 kb on + strand, within Dshi_2814 at 2970.286 kb on - strand, within Dshi_2814 at 2970.286 kb on - strand, within Dshi_2814 at 2970.318 kb on - strand, within Dshi_2814 at 2970.552 kb on - strand, within Dshi_2814 at 2970.552 kb on - strand, within Dshi_2814 at 2970.607 kb on - strand, within Dshi_2814 at 2970.607 kb on - strand, within Dshi_2814 at 2970.622 kb on + strand, within Dshi_2814
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 40 mM remove 2,967,666 - Dshi_2810 0.73 -0.6 2,967,666 - Dshi_2810 0.73 -2.2 2,967,750 + -0.4 2,967,750 + +0.6 2,967,881 - Dshi_2811 0.21 -2.1 2,967,931 + Dshi_2811 0.31 +2.4 2,968,007 - Dshi_2811 0.46 +0.1 2,968,015 + Dshi_2811 0.47 +0.6 2,968,062 - Dshi_2811 0.57 +1.8 2,968,224 - Dshi_2811 0.88 +2.1 2,968,253 - -1.0 2,968,310 - -1.2 2,968,332 + +0.5 2,968,340 + Dshi_2812 0.10 +0.3 2,968,635 + +1.1 2,968,732 - -0.2 2,968,754 - -0.4 2,968,754 - +2.3 2,968,843 + Dshi_2813 0.17 +0.1 2,968,846 - Dshi_2813 0.17 +0.4 2,968,848 + Dshi_2813 0.17 +0.4 2,968,914 - Dshi_2813 0.23 +1.4 2,969,021 - Dshi_2813 0.33 +1.4 2,969,030 - Dshi_2813 0.34 -1.7 2,969,102 + Dshi_2813 0.40 +0.0 2,969,177 + Dshi_2813 0.47 +0.4 2,969,363 + +0.8 2,969,424 - Dshi_2813 0.70 +0.4 2,969,439 + Dshi_2813 0.71 +0.8 2,969,439 + Dshi_2813 0.71 -2.9 2,969,487 + Dshi_2813 0.76 -0.3 2,969,487 + Dshi_2813 0.76 +2.4 2,969,487 + Dshi_2813 0.76 -0.3 2,969,490 - Dshi_2813 0.76 -0.4 2,969,495 - Dshi_2813 0.76 +2.4 2,969,495 - Dshi_2813 0.76 -0.7 2,969,513 + Dshi_2813 0.78 +1.0 2,969,521 - Dshi_2813 0.79 +0.1 2,969,521 - Dshi_2813 0.79 -1.3 2,969,573 + Dshi_2813 0.84 -1.1 2,969,603 + Dshi_2813 0.86 -0.7 2,969,661 - -1.4 2,969,784 + +4.3 2,969,815 + +1.4 2,969,846 - -0.2 2,969,846 - +2.1 2,969,847 + -1.5 2,969,847 + +0.4 2,969,964 + Dshi_2814 0.19 +2.4 2,970,104 + Dshi_2814 0.33 +0.3 2,970,115 + Dshi_2814 0.34 -1.7 2,970,123 - Dshi_2814 0.35 +2.4 2,970,282 + Dshi_2814 0.50 -0.2 2,970,286 - Dshi_2814 0.50 -0.3 2,970,286 - Dshi_2814 0.50 +0.6 2,970,318 - Dshi_2814 0.53 -0.6 2,970,552 - Dshi_2814 0.76 -2.6 2,970,552 - Dshi_2814 0.76 -0.2 2,970,607 - Dshi_2814 0.81 +1.0 2,970,607 - Dshi_2814 0.81 +1.1 2,970,622 + Dshi_2814 0.83 -1.3
Or see this region's nucleotide sequence