Experiment: m.b. Cholin acetate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2019 and Dshi_2020 are separated by 101 nucleotides Dshi_2020 and Dshi_2021 are separated by 228 nucleotides
Dshi_2019: Dshi_2019 - ferredoxin-dependent glutamate synthase (RefSeq), at 2,137,715 to 2,139,322
_2019
Dshi_2020: Dshi_2020 - phosphoglucomutase/phosphomannomutase alpha/beta/alpha domain I (RefSeq), at 2,139,424 to 2,141,055
_2020
Dshi_2021: Dshi_2021 - extracellular solute-binding protein family 1 (RefSeq), at 2,141,284 to 2,142,312
_2021
Position (kb)
2139
2140
2141
2142 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2138.603 kb on - strand, within Dshi_2019 at 2138.702 kb on - strand, within Dshi_2019 at 2138.725 kb on - strand, within Dshi_2019 at 2138.764 kb on + strand, within Dshi_2019 at 2138.836 kb on + strand, within Dshi_2019 at 2139.120 kb on + strand, within Dshi_2019 at 2139.190 kb on + strand at 2139.214 kb on + strand at 2139.224 kb on - strand at 2139.370 kb on + strand at 2139.410 kb on + strand at 2139.653 kb on - strand, within Dshi_2020 at 2139.749 kb on - strand, within Dshi_2020 at 2139.788 kb on + strand, within Dshi_2020 at 2139.901 kb on - strand, within Dshi_2020 at 2139.904 kb on + strand, within Dshi_2020 at 2139.937 kb on + strand, within Dshi_2020 at 2140.096 kb on - strand, within Dshi_2020 at 2140.291 kb on + strand, within Dshi_2020 at 2140.309 kb on - strand, within Dshi_2020 at 2140.318 kb on + strand, within Dshi_2020 at 2140.331 kb on - strand, within Dshi_2020 at 2140.367 kb on - strand, within Dshi_2020 at 2140.393 kb on + strand, within Dshi_2020 at 2140.488 kb on - strand, within Dshi_2020 at 2140.497 kb on - strand, within Dshi_2020 at 2140.523 kb on + strand, within Dshi_2020 at 2140.568 kb on - strand, within Dshi_2020 at 2140.644 kb on + strand, within Dshi_2020 at 2140.670 kb on - strand, within Dshi_2020 at 2140.678 kb on - strand, within Dshi_2020 at 2140.712 kb on - strand, within Dshi_2020 at 2140.748 kb on - strand, within Dshi_2020 at 2140.835 kb on + strand, within Dshi_2020 at 2140.840 kb on + strand, within Dshi_2020 at 2140.985 kb on + strand at 2140.985 kb on + strand at 2140.993 kb on - strand at 2140.993 kb on - strand at 2140.995 kb on + strand at 2141.003 kb on - strand at 2141.032 kb on + strand at 2141.077 kb on - strand at 2141.140 kb on + strand at 2141.157 kb on + strand at 2141.167 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 40 mM remove 2,138,603 - Dshi_2019 0.55 +1.2 2,138,702 - Dshi_2019 0.61 -0.6 2,138,725 - Dshi_2019 0.63 -1.1 2,138,764 + Dshi_2019 0.65 -1.3 2,138,836 + Dshi_2019 0.70 +1.7 2,139,120 + Dshi_2019 0.87 +0.8 2,139,190 + +1.4 2,139,214 + -0.2 2,139,224 - -2.4 2,139,370 + +0.9 2,139,410 + -1.0 2,139,653 - Dshi_2020 0.14 -0.7 2,139,749 - Dshi_2020 0.20 +1.5 2,139,788 + Dshi_2020 0.22 -0.8 2,139,901 - Dshi_2020 0.29 +0.3 2,139,904 + Dshi_2020 0.29 +0.7 2,139,937 + Dshi_2020 0.31 +0.9 2,140,096 - Dshi_2020 0.41 +2.3 2,140,291 + Dshi_2020 0.53 +0.5 2,140,309 - Dshi_2020 0.54 +1.3 2,140,318 + Dshi_2020 0.55 +0.5 2,140,331 - Dshi_2020 0.56 +1.9 2,140,367 - Dshi_2020 0.58 +0.0 2,140,393 + Dshi_2020 0.59 +0.0 2,140,488 - Dshi_2020 0.65 -1.5 2,140,497 - Dshi_2020 0.66 -1.5 2,140,523 + Dshi_2020 0.67 +0.6 2,140,568 - Dshi_2020 0.70 +0.7 2,140,644 + Dshi_2020 0.75 +1.2 2,140,670 - Dshi_2020 0.76 +1.1 2,140,678 - Dshi_2020 0.77 -1.1 2,140,712 - Dshi_2020 0.79 +0.2 2,140,748 - Dshi_2020 0.81 +1.6 2,140,835 + Dshi_2020 0.86 -1.4 2,140,840 + Dshi_2020 0.87 -0.8 2,140,985 + -1.0 2,140,985 + +1.1 2,140,993 - +0.1 2,140,993 - -0.7 2,140,995 + -1.5 2,141,003 - +0.8 2,141,032 + -0.8 2,141,077 - +0.4 2,141,140 + +0.7 2,141,157 + +2.9 2,141,167 - +0.6
Or see this region's nucleotide sequence