Experiment: m.b. Cholin acetate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1893 and Dshi_1894 are separated by 11 nucleotides Dshi_1894 and Dshi_1895 are separated by 18 nucleotides Dshi_1895 and Dshi_1896 are separated by 28 nucleotides
Dshi_1893: Dshi_1893 - PAS/PAC sensor hybrid histidine kinase (RefSeq), at 1,970,454 to 1,973,216
_1893
Dshi_1894: Dshi_1894 - two component transcriptional regulator, LuxR family (RefSeq), at 1,973,228 to 1,973,842
_1894
Dshi_1895: Dshi_1895 - Hemolysin-type calcium-binding region (RefSeq), at 1,973,861 to 1,976,413
_1895
Dshi_1896: Dshi_1896 - GDP-mannose 6-dehydrogenase (RefSeq), at 1,976,442 to 1,977,851
_1896
Position (kb)
1973
1974
1975
1976
1977 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1972.951 kb on + strand at 1973.204 kb on - strand at 1973.229 kb on + strand at 1973.383 kb on + strand, within Dshi_1894 at 1973.413 kb on + strand, within Dshi_1894 at 1973.538 kb on - strand, within Dshi_1894 at 1973.590 kb on + strand, within Dshi_1894 at 1973.592 kb on + strand, within Dshi_1894 at 1973.700 kb on - strand, within Dshi_1894 at 1973.748 kb on + strand, within Dshi_1894 at 1973.836 kb on + strand at 1973.857 kb on - strand at 1973.877 kb on - strand at 1974.046 kb on + strand at 1974.046 kb on + strand at 1974.180 kb on + strand, within Dshi_1895 at 1974.225 kb on - strand, within Dshi_1895 at 1974.236 kb on - strand, within Dshi_1895 at 1974.398 kb on - strand, within Dshi_1895 at 1974.434 kb on + strand, within Dshi_1895 at 1974.622 kb on + strand, within Dshi_1895 at 1974.669 kb on + strand, within Dshi_1895 at 1974.803 kb on + strand, within Dshi_1895 at 1974.858 kb on - strand, within Dshi_1895 at 1974.881 kb on - strand, within Dshi_1895 at 1974.896 kb on + strand, within Dshi_1895 at 1974.969 kb on - strand, within Dshi_1895 at 1974.990 kb on + strand, within Dshi_1895 at 1974.990 kb on + strand, within Dshi_1895 at 1975.050 kb on - strand, within Dshi_1895 at 1975.083 kb on - strand, within Dshi_1895 at 1975.117 kb on + strand, within Dshi_1895 at 1975.136 kb on - strand, within Dshi_1895 at 1975.213 kb on + strand, within Dshi_1895 at 1975.221 kb on - strand, within Dshi_1895 at 1975.221 kb on - strand, within Dshi_1895 at 1975.252 kb on - strand, within Dshi_1895 at 1975.410 kb on + strand, within Dshi_1895 at 1975.418 kb on - strand, within Dshi_1895 at 1975.418 kb on - strand, within Dshi_1895 at 1975.476 kb on + strand, within Dshi_1895 at 1975.476 kb on + strand, within Dshi_1895 at 1975.484 kb on - strand, within Dshi_1895 at 1975.505 kb on + strand, within Dshi_1895 at 1975.505 kb on + strand, within Dshi_1895 at 1975.530 kb on + strand, within Dshi_1895 at 1975.571 kb on + strand, within Dshi_1895 at 1975.657 kb on - strand, within Dshi_1895 at 1975.727 kb on - strand, within Dshi_1895 at 1975.727 kb on - strand, within Dshi_1895 at 1975.783 kb on + strand, within Dshi_1895 at 1975.860 kb on - strand, within Dshi_1895 at 1975.939 kb on - strand, within Dshi_1895 at 1975.968 kb on + strand, within Dshi_1895 at 1975.975 kb on + strand, within Dshi_1895 at 1976.061 kb on - strand, within Dshi_1895 at 1976.061 kb on - strand, within Dshi_1895 at 1976.061 kb on - strand, within Dshi_1895 at 1976.066 kb on + strand, within Dshi_1895 at 1976.066 kb on + strand, within Dshi_1895 at 1976.074 kb on - strand, within Dshi_1895 at 1976.106 kb on - strand, within Dshi_1895 at 1976.106 kb on - strand, within Dshi_1895 at 1976.129 kb on - strand, within Dshi_1895 at 1976.156 kb on + strand, within Dshi_1895 at 1976.169 kb on + strand at 1976.169 kb on - strand at 1976.209 kb on + strand at 1976.278 kb on - strand at 1976.386 kb on - strand at 1976.400 kb on - strand at 1976.404 kb on + strand at 1976.514 kb on + strand at 1976.522 kb on - strand at 1976.523 kb on - strand at 1976.665 kb on - strand, within Dshi_1896 at 1976.665 kb on - strand, within Dshi_1896 at 1976.806 kb on - strand, within Dshi_1896 at 1976.809 kb on + strand, within Dshi_1896 at 1976.931 kb on + strand, within Dshi_1896 at 1976.932 kb on + strand, within Dshi_1896 at 1976.978 kb on + strand, within Dshi_1896 at 1976.978 kb on + strand, within Dshi_1896 at 1977.140 kb on - strand, within Dshi_1896 at 1977.159 kb on + strand, within Dshi_1896 at 1977.262 kb on + strand, within Dshi_1896
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 40 mM remove 1,972,951 + -0.7 1,973,204 - +1.1 1,973,229 + -1.2 1,973,383 + Dshi_1894 0.25 -2.1 1,973,413 + Dshi_1894 0.30 +1.7 1,973,538 - Dshi_1894 0.50 +1.4 1,973,590 + Dshi_1894 0.59 +0.2 1,973,592 + Dshi_1894 0.59 -0.6 1,973,700 - Dshi_1894 0.77 -1.7 1,973,748 + Dshi_1894 0.85 -1.0 1,973,836 + +0.1 1,973,857 - +0.6 1,973,877 - -0.9 1,974,046 + +0.6 1,974,046 + +0.1 1,974,180 + Dshi_1895 0.12 -0.2 1,974,225 - Dshi_1895 0.14 -0.4 1,974,236 - Dshi_1895 0.15 +0.4 1,974,398 - Dshi_1895 0.21 -1.5 1,974,434 + Dshi_1895 0.22 +1.0 1,974,622 + Dshi_1895 0.30 -0.4 1,974,669 + Dshi_1895 0.32 -0.6 1,974,803 + Dshi_1895 0.37 +0.1 1,974,858 - Dshi_1895 0.39 -1.2 1,974,881 - Dshi_1895 0.40 +0.2 1,974,896 + Dshi_1895 0.41 -0.1 1,974,969 - Dshi_1895 0.43 -0.8 1,974,990 + Dshi_1895 0.44 +0.3 1,974,990 + Dshi_1895 0.44 +1.1 1,975,050 - Dshi_1895 0.47 -1.0 1,975,083 - Dshi_1895 0.48 +0.3 1,975,117 + Dshi_1895 0.49 -2.1 1,975,136 - Dshi_1895 0.50 +0.9 1,975,213 + Dshi_1895 0.53 +0.3 1,975,221 - Dshi_1895 0.53 -1.1 1,975,221 - Dshi_1895 0.53 -0.8 1,975,252 - Dshi_1895 0.54 -1.2 1,975,410 + Dshi_1895 0.61 +0.5 1,975,418 - Dshi_1895 0.61 -0.5 1,975,418 - Dshi_1895 0.61 -0.3 1,975,476 + Dshi_1895 0.63 +0.5 1,975,476 + Dshi_1895 0.63 +0.4 1,975,484 - Dshi_1895 0.64 -2.3 1,975,505 + Dshi_1895 0.64 -1.3 1,975,505 + Dshi_1895 0.64 +1.8 1,975,530 + Dshi_1895 0.65 -0.8 1,975,571 + Dshi_1895 0.67 +0.2 1,975,657 - Dshi_1895 0.70 +0.7 1,975,727 - Dshi_1895 0.73 +1.1 1,975,727 - Dshi_1895 0.73 -1.3 1,975,783 + Dshi_1895 0.75 -1.8 1,975,860 - Dshi_1895 0.78 -0.6 1,975,939 - Dshi_1895 0.81 +0.1 1,975,968 + Dshi_1895 0.83 +0.3 1,975,975 + Dshi_1895 0.83 -1.6 1,976,061 - Dshi_1895 0.86 -0.8 1,976,061 - Dshi_1895 0.86 -1.2 1,976,061 - Dshi_1895 0.86 -2.1 1,976,066 + Dshi_1895 0.86 +0.1 1,976,066 + Dshi_1895 0.86 +0.5 1,976,074 - Dshi_1895 0.87 -2.3 1,976,106 - Dshi_1895 0.88 -4.2 1,976,106 - Dshi_1895 0.88 +0.6 1,976,129 - Dshi_1895 0.89 -1.5 1,976,156 + Dshi_1895 0.90 +2.6 1,976,169 + +0.9 1,976,169 - -1.2 1,976,209 + +0.1 1,976,278 - -0.5 1,976,386 - +0.2 1,976,400 - -1.5 1,976,404 + -0.8 1,976,514 + +0.1 1,976,522 - -1.3 1,976,523 - -0.5 1,976,665 - Dshi_1896 0.16 -0.2 1,976,665 - Dshi_1896 0.16 +0.2 1,976,806 - Dshi_1896 0.26 -0.8 1,976,809 + Dshi_1896 0.26 -3.0 1,976,931 + Dshi_1896 0.35 +0.7 1,976,932 + Dshi_1896 0.35 -1.6 1,976,978 + Dshi_1896 0.38 -0.8 1,976,978 + Dshi_1896 0.38 +0.6 1,977,140 - Dshi_1896 0.50 -0.4 1,977,159 + Dshi_1896 0.51 -1.0 1,977,262 + Dshi_1896 0.58 -0.3
Or see this region's nucleotide sequence