Experiment: m.b. Cholin acetate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1482 and Dshi_R0028 are separated by 51 nucleotides Dshi_R0028 and Dshi_1483 are separated by 43 nucleotides Dshi_1483 and Dshi_1484 are separated by 163 nucleotides Dshi_1484 and Dshi_1485 are separated by 187 nucleotides
Dshi_1482: Dshi_1482 - TRAP dicarboxylate transporter, DctM subunit (RefSeq), at 1,536,841 to 1,538,193
_1482
Dshi_R0028: Dshi_R0028 - tRNA-Leu (RefSeq), at 1,538,245 to 1,538,331
_R0028
Dshi_1483: Dshi_1483 - hypothetical protein (RefSeq), at 1,538,375 to 1,538,743
_1483
Dshi_1484: Dshi_1484 - hypothetical protein (RefSeq), at 1,538,907 to 1,539,512
_1484
Dshi_1485: Dshi_1485 - FAD dependent oxidoreductase (RefSeq), at 1,539,700 to 1,542,114
_1485
Position (kb)
1538
1539 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1537.542 kb on + strand, within Dshi_1482 at 1537.553 kb on + strand, within Dshi_1482 at 1537.840 kb on - strand, within Dshi_1482 at 1537.966 kb on - strand, within Dshi_1482 at 1538.091 kb on - strand at 1538.384 kb on + strand at 1538.669 kb on - strand, within Dshi_1483 at 1539.008 kb on - strand, within Dshi_1484 at 1539.146 kb on + strand, within Dshi_1484 at 1539.254 kb on + strand, within Dshi_1484 at 1539.426 kb on + strand, within Dshi_1484 at 1539.487 kb on + strand at 1539.491 kb on + strand at 1539.612 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 40 mM remove 1,537,542 + Dshi_1482 0.52 +2.2 1,537,553 + Dshi_1482 0.53 +1.8 1,537,840 - Dshi_1482 0.74 +0.1 1,537,966 - Dshi_1482 0.83 +2.6 1,538,091 - -0.4 1,538,384 + +1.4 1,538,669 - Dshi_1483 0.80 -0.3 1,539,008 - Dshi_1484 0.17 -2.9 1,539,146 + Dshi_1484 0.39 +0.8 1,539,254 + Dshi_1484 0.57 +1.8 1,539,426 + Dshi_1484 0.86 -0.5 1,539,487 + -0.7 1,539,491 + +0.8 1,539,612 - -0.2
Or see this region's nucleotide sequence