Experiment: m.b. Cholin acetate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1438 and Dshi_1439 are separated by 51 nucleotides Dshi_1439 and Dshi_1440 are separated by 87 nucleotides
Dshi_1438: Dshi_1438 - Substrate-binding region of ABC-type glycine betaine transport system (RefSeq), at 1,493,812 to 1,494,777
_1438
Dshi_1439: Dshi_1439 - pyridoxal phosphate-dependent enzyme, D-cysteine desulfhydrase family (RefSeq), at 1,494,829 to 1,495,872
_1439
Dshi_1440: Dshi_1440 - transcriptional regulator, LysR family (RefSeq), at 1,495,960 to 1,496,892
_1440
Position (kb)
1494
1495
1496 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1493.835 kb on - strand at 1493.857 kb on - strand at 1493.897 kb on - strand at 1493.920 kb on - strand, within Dshi_1438 at 1493.924 kb on - strand, within Dshi_1438 at 1493.995 kb on - strand, within Dshi_1438 at 1494.012 kb on + strand, within Dshi_1438 at 1494.012 kb on - strand, within Dshi_1438 at 1494.131 kb on + strand, within Dshi_1438 at 1494.139 kb on - strand, within Dshi_1438 at 1494.160 kb on - strand, within Dshi_1438 at 1494.175 kb on + strand, within Dshi_1438 at 1494.175 kb on + strand, within Dshi_1438 at 1494.175 kb on + strand, within Dshi_1438 at 1494.183 kb on - strand, within Dshi_1438 at 1494.183 kb on - strand, within Dshi_1438 at 1494.231 kb on - strand, within Dshi_1438 at 1494.243 kb on + strand, within Dshi_1438 at 1494.279 kb on - strand, within Dshi_1438 at 1494.300 kb on - strand, within Dshi_1438 at 1494.374 kb on - strand, within Dshi_1438 at 1494.381 kb on + strand, within Dshi_1438 at 1494.408 kb on - strand, within Dshi_1438 at 1494.458 kb on - strand, within Dshi_1438 at 1494.617 kb on + strand, within Dshi_1438 at 1494.640 kb on - strand, within Dshi_1438 at 1494.651 kb on - strand, within Dshi_1438 at 1494.682 kb on - strand at 1494.697 kb on + strand at 1494.748 kb on - strand at 1494.750 kb on + strand at 1494.770 kb on + strand at 1494.838 kb on - strand at 1494.852 kb on + strand at 1494.951 kb on - strand, within Dshi_1439 at 1494.977 kb on + strand, within Dshi_1439 at 1494.985 kb on - strand, within Dshi_1439 at 1495.020 kb on + strand, within Dshi_1439 at 1495.035 kb on + strand, within Dshi_1439 at 1495.035 kb on + strand, within Dshi_1439 at 1495.038 kb on - strand, within Dshi_1439 at 1495.048 kb on - strand, within Dshi_1439 at 1495.127 kb on + strand, within Dshi_1439 at 1495.139 kb on - strand, within Dshi_1439 at 1495.146 kb on - strand, within Dshi_1439 at 1495.242 kb on - strand, within Dshi_1439 at 1495.308 kb on - strand, within Dshi_1439 at 1495.412 kb on + strand, within Dshi_1439 at 1495.433 kb on + strand, within Dshi_1439 at 1495.438 kb on + strand, within Dshi_1439 at 1495.472 kb on + strand, within Dshi_1439 at 1495.517 kb on - strand, within Dshi_1439 at 1495.537 kb on - strand, within Dshi_1439 at 1495.537 kb on - strand, within Dshi_1439 at 1495.617 kb on + strand, within Dshi_1439 at 1495.755 kb on - strand, within Dshi_1439 at 1495.760 kb on - strand, within Dshi_1439 at 1495.799 kb on - strand at 1495.848 kb on + strand at 1495.857 kb on + strand at 1495.857 kb on - strand at 1495.865 kb on - strand at 1495.867 kb on + strand at 1495.867 kb on + strand at 1495.898 kb on - strand at 1495.902 kb on + strand at 1495.975 kb on + strand at 1496.073 kb on - strand, within Dshi_1440 at 1496.090 kb on - strand, within Dshi_1440 at 1496.148 kb on + strand, within Dshi_1440 at 1496.154 kb on + strand, within Dshi_1440 at 1496.296 kb on + strand, within Dshi_1440 at 1496.375 kb on + strand, within Dshi_1440 at 1496.375 kb on - strand, within Dshi_1440 at 1496.424 kb on - strand, within Dshi_1440 at 1496.594 kb on + strand, within Dshi_1440 at 1496.625 kb on + strand, within Dshi_1440 at 1496.703 kb on + strand, within Dshi_1440 at 1496.747 kb on + strand, within Dshi_1440
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 40 mM remove 1,493,835 - -0.6 1,493,857 - -2.9 1,493,897 - -0.5 1,493,920 - Dshi_1438 0.11 +0.1 1,493,924 - Dshi_1438 0.12 -0.0 1,493,995 - Dshi_1438 0.19 +0.4 1,494,012 + Dshi_1438 0.21 +0.6 1,494,012 - Dshi_1438 0.21 +0.4 1,494,131 + Dshi_1438 0.33 +1.0 1,494,139 - Dshi_1438 0.34 +0.8 1,494,160 - Dshi_1438 0.36 +0.3 1,494,175 + Dshi_1438 0.38 -0.3 1,494,175 + Dshi_1438 0.38 +0.5 1,494,175 + Dshi_1438 0.38 +0.3 1,494,183 - Dshi_1438 0.38 +1.5 1,494,183 - Dshi_1438 0.38 -0.1 1,494,231 - Dshi_1438 0.43 +0.6 1,494,243 + Dshi_1438 0.45 +0.3 1,494,279 - Dshi_1438 0.48 -0.0 1,494,300 - Dshi_1438 0.51 +1.4 1,494,374 - Dshi_1438 0.58 +0.8 1,494,381 + Dshi_1438 0.59 -0.4 1,494,408 - Dshi_1438 0.62 +0.7 1,494,458 - Dshi_1438 0.67 -0.1 1,494,617 + Dshi_1438 0.83 -0.4 1,494,640 - Dshi_1438 0.86 -0.4 1,494,651 - Dshi_1438 0.87 -1.0 1,494,682 - -1.4 1,494,697 + +0.3 1,494,748 - -0.4 1,494,750 + -0.5 1,494,770 + +0.8 1,494,838 - -0.1 1,494,852 + -0.0 1,494,951 - Dshi_1439 0.12 -1.0 1,494,977 + Dshi_1439 0.14 -0.1 1,494,985 - Dshi_1439 0.15 +0.8 1,495,020 + Dshi_1439 0.18 +1.2 1,495,035 + Dshi_1439 0.20 +1.7 1,495,035 + Dshi_1439 0.20 +0.1 1,495,038 - Dshi_1439 0.20 +0.9 1,495,048 - Dshi_1439 0.21 -1.7 1,495,127 + Dshi_1439 0.29 -0.1 1,495,139 - Dshi_1439 0.30 -0.3 1,495,146 - Dshi_1439 0.30 -0.5 1,495,242 - Dshi_1439 0.40 +0.2 1,495,308 - Dshi_1439 0.46 +0.1 1,495,412 + Dshi_1439 0.56 +0.4 1,495,433 + Dshi_1439 0.58 +0.4 1,495,438 + Dshi_1439 0.58 -0.8 1,495,472 + Dshi_1439 0.62 +0.3 1,495,517 - Dshi_1439 0.66 +0.5 1,495,537 - Dshi_1439 0.68 +0.6 1,495,537 - Dshi_1439 0.68 -0.1 1,495,617 + Dshi_1439 0.75 -1.3 1,495,755 - Dshi_1439 0.89 -1.6 1,495,760 - Dshi_1439 0.89 +0.3 1,495,799 - -0.1 1,495,848 + +0.2 1,495,857 + +0.0 1,495,857 - -0.1 1,495,865 - +3.0 1,495,867 + -0.2 1,495,867 + -0.3 1,495,898 - -1.3 1,495,902 + +1.1 1,495,975 + +2.0 1,496,073 - Dshi_1440 0.12 +0.2 1,496,090 - Dshi_1440 0.14 -1.7 1,496,148 + Dshi_1440 0.20 +0.3 1,496,154 + Dshi_1440 0.21 -0.4 1,496,296 + Dshi_1440 0.36 -0.8 1,496,375 + Dshi_1440 0.44 +0.7 1,496,375 - Dshi_1440 0.44 -0.4 1,496,424 - Dshi_1440 0.50 -0.2 1,496,594 + Dshi_1440 0.68 -0.1 1,496,625 + Dshi_1440 0.71 +0.7 1,496,703 + Dshi_1440 0.80 +0.6 1,496,747 + Dshi_1440 0.84 -0.7
Or see this region's nucleotide sequence