Experiment: m.b. Cholin acetate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0910 and Dshi_0911 overlap by 4 nucleotides Dshi_0911 and Dshi_0912 overlap by 4 nucleotides Dshi_0912 and Dshi_0913 are separated by 3 nucleotides Dshi_0913 and Dshi_0914 overlap by 17 nucleotides
Dshi_0910: Dshi_0910 - UBA/THIF-type NAD/FAD binding protein (RefSeq), at 933,279 to 934,244
_0910
Dshi_0911: Dshi_0911 - Thiamine-phosphate diphosphorylase (RefSeq), at 934,241 to 934,840
_0911
Dshi_0912: Dshi_0912 - thiazole biosynthesis family protein (RefSeq), at 934,837 to 935,631
_0912
Dshi_0913: Dshi_0913 - thiamine biosynthesis protein ThiS (RefSeq), at 935,635 to 935,829
_0913
Dshi_0914: Dshi_0914 - glycine oxidase ThiO (RefSeq), at 935,813 to 936,790
_0914
Position (kb) 934
935
936 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 933.867 kb on - strand, within Dshi_0910 at 934.109 kb on - strand, within Dshi_0910 at 934.125 kb on + strand, within Dshi_0910 at 934.133 kb on - strand, within Dshi_0910 at 934.215 kb on - strand at 934.281 kb on + strand at 934.676 kb on + strand, within Dshi_0911 at 934.761 kb on + strand, within Dshi_0911 at 935.087 kb on - strand, within Dshi_0912 at 935.127 kb on + strand, within Dshi_0912 at 935.169 kb on - strand, within Dshi_0912 at 935.274 kb on + strand, within Dshi_0912 at 935.376 kb on - strand, within Dshi_0912 at 935.457 kb on - strand, within Dshi_0912 at 935.621 kb on + strand at 935.621 kb on + strand at 935.694 kb on - strand, within Dshi_0913 at 935.884 kb on + strand at 936.063 kb on - strand, within Dshi_0914 at 936.195 kb on - strand, within Dshi_0914 at 936.394 kb on - strand, within Dshi_0914 at 936.573 kb on + strand, within Dshi_0914 at 936.607 kb on - strand, within Dshi_0914
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 40 mM remove 933,867 - Dshi_0910 0.61 -1.6 934,109 - Dshi_0910 0.86 -0.7 934,125 + Dshi_0910 0.88 +0.6 934,133 - Dshi_0910 0.88 +0.8 934,215 - -1.5 934,281 + +3.2 934,676 + Dshi_0911 0.72 +0.4 934,761 + Dshi_0911 0.87 +3.0 935,087 - Dshi_0912 0.31 -2.2 935,127 + Dshi_0912 0.36 +2.4 935,169 - Dshi_0912 0.42 -0.9 935,274 + Dshi_0912 0.55 -0.9 935,376 - Dshi_0912 0.68 +1.9 935,457 - Dshi_0912 0.78 +1.4 935,621 + -0.3 935,621 + -1.6 935,694 - Dshi_0913 0.30 -0.2 935,884 + -0.6 936,063 - Dshi_0914 0.26 +1.4 936,195 - Dshi_0914 0.39 +0.4 936,394 - Dshi_0914 0.59 +2.4 936,573 + Dshi_0914 0.78 +2.4 936,607 - Dshi_0914 0.81 -0.2
Or see this region's nucleotide sequence