Experiment: m.b. Cholin acetate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0689 and Dshi_0690 overlap by 4 nucleotides Dshi_0690 and Dshi_0691 overlap by 4 nucleotides Dshi_0691 and Dshi_0692 overlap by 4 nucleotides
Dshi_0689: Dshi_0689 - isocitrate lyase family protein (RefSeq), at 694,835 to 695,713
_0689
Dshi_0690: Dshi_0690 - isochorismatase hydrolase (RefSeq), at 695,710 to 696,312
_0690
Dshi_0691: Dshi_0691 - 5-oxoprolinase (ATP-hydrolyzing) (RefSeq), at 696,309 to 698,378
_0691
Dshi_0692: Dshi_0692 - 5-oxoprolinase (ATP-hydrolyzing) (RefSeq), at 698,375 to 700,072
_0692
Position (kb)
696
697
698
699 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 695.323 kb on + strand, within Dshi_0689 at 695.367 kb on + strand, within Dshi_0689 at 695.375 kb on - strand, within Dshi_0689 at 695.375 kb on - strand, within Dshi_0689 at 695.377 kb on + strand, within Dshi_0689 at 695.435 kb on - strand, within Dshi_0689 at 695.458 kb on - strand, within Dshi_0689 at 695.492 kb on - strand, within Dshi_0689 at 695.545 kb on - strand, within Dshi_0689 at 695.566 kb on + strand, within Dshi_0689 at 695.568 kb on + strand, within Dshi_0689 at 695.593 kb on + strand, within Dshi_0689 at 695.767 kb on + strand at 695.775 kb on - strand, within Dshi_0690 at 695.775 kb on - strand, within Dshi_0690 at 695.793 kb on + strand, within Dshi_0690 at 695.852 kb on - strand, within Dshi_0690 at 695.861 kb on + strand, within Dshi_0690 at 695.944 kb on + strand, within Dshi_0690 at 695.944 kb on + strand, within Dshi_0690 at 695.952 kb on - strand, within Dshi_0690 at 695.957 kb on - strand, within Dshi_0690 at 695.994 kb on + strand, within Dshi_0690 at 696.001 kb on - strand, within Dshi_0690 at 696.042 kb on - strand, within Dshi_0690 at 696.232 kb on + strand, within Dshi_0690 at 696.236 kb on + strand, within Dshi_0690 at 696.340 kb on + strand at 696.369 kb on - strand at 696.421 kb on - strand at 696.447 kb on - strand at 696.447 kb on - strand at 696.544 kb on + strand, within Dshi_0691 at 696.622 kb on + strand, within Dshi_0691 at 696.628 kb on + strand, within Dshi_0691 at 696.628 kb on + strand, within Dshi_0691 at 696.708 kb on + strand at 696.729 kb on + strand, within Dshi_0691 at 696.755 kb on + strand, within Dshi_0691 at 696.953 kb on - strand, within Dshi_0691 at 696.962 kb on + strand, within Dshi_0691 at 696.988 kb on + strand, within Dshi_0691 at 696.998 kb on - strand, within Dshi_0691 at 696.998 kb on - strand, within Dshi_0691 at 697.116 kb on - strand, within Dshi_0691 at 697.216 kb on + strand, within Dshi_0691 at 697.321 kb on - strand, within Dshi_0691 at 697.377 kb on - strand, within Dshi_0691 at 697.417 kb on + strand, within Dshi_0691 at 697.440 kb on + strand, within Dshi_0691 at 697.448 kb on - strand, within Dshi_0691 at 697.448 kb on - strand, within Dshi_0691 at 697.476 kb on + strand, within Dshi_0691 at 697.500 kb on + strand, within Dshi_0691 at 697.608 kb on + strand, within Dshi_0691 at 697.635 kb on - strand, within Dshi_0691 at 697.663 kb on + strand, within Dshi_0691 at 697.707 kb on + strand, within Dshi_0691 at 697.707 kb on + strand, within Dshi_0691 at 697.721 kb on - strand, within Dshi_0691 at 697.785 kb on - strand, within Dshi_0691 at 697.851 kb on - strand, within Dshi_0691 at 697.946 kb on + strand, within Dshi_0691 at 697.977 kb on + strand, within Dshi_0691 at 698.072 kb on + strand, within Dshi_0691 at 698.074 kb on + strand, within Dshi_0691 at 698.080 kb on - strand, within Dshi_0691 at 698.144 kb on - strand, within Dshi_0691 at 698.237 kb on + strand at 698.422 kb on + strand at 698.489 kb on + strand at 698.757 kb on - strand, within Dshi_0692 at 698.825 kb on + strand at 698.928 kb on + strand, within Dshi_0692 at 698.936 kb on - strand, within Dshi_0692 at 698.936 kb on - strand, within Dshi_0692 at 698.949 kb on + strand, within Dshi_0692 at 698.975 kb on + strand, within Dshi_0692 at 699.101 kb on + strand, within Dshi_0692 at 699.107 kb on - strand, within Dshi_0692 at 699.117 kb on - strand, within Dshi_0692 at 699.195 kb on + strand, within Dshi_0692 at 699.213 kb on - strand, within Dshi_0692
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 40 mM remove 695,323 + Dshi_0689 0.56 +1.2 695,367 + Dshi_0689 0.61 +0.1 695,375 - Dshi_0689 0.61 +0.7 695,375 - Dshi_0689 0.61 +0.0 695,377 + Dshi_0689 0.62 +1.4 695,435 - Dshi_0689 0.68 -1.0 695,458 - Dshi_0689 0.71 -0.7 695,492 - Dshi_0689 0.75 +2.4 695,545 - Dshi_0689 0.81 +1.0 695,566 + Dshi_0689 0.83 -1.9 695,568 + Dshi_0689 0.83 +0.2 695,593 + Dshi_0689 0.86 -0.7 695,767 + +0.2 695,775 - Dshi_0690 0.11 -0.4 695,775 - Dshi_0690 0.11 -2.2 695,793 + Dshi_0690 0.14 +2.0 695,852 - Dshi_0690 0.24 -2.4 695,861 + Dshi_0690 0.25 -1.0 695,944 + Dshi_0690 0.39 +2.4 695,944 + Dshi_0690 0.39 +2.0 695,952 - Dshi_0690 0.40 +1.5 695,957 - Dshi_0690 0.41 +0.9 695,994 + Dshi_0690 0.47 +1.7 696,001 - Dshi_0690 0.48 +3.1 696,042 - Dshi_0690 0.55 +1.1 696,232 + Dshi_0690 0.87 +0.4 696,236 + Dshi_0690 0.87 +0.1 696,340 + +0.8 696,369 - +1.4 696,421 - +1.4 696,447 - +0.2 696,447 - +0.8 696,544 + Dshi_0691 0.11 +0.7 696,622 + Dshi_0691 0.15 +0.0 696,628 + Dshi_0691 0.15 +0.8 696,628 + Dshi_0691 0.15 -2.2 696,708 + +2.4 696,729 + Dshi_0691 0.20 +0.8 696,755 + Dshi_0691 0.22 +2.4 696,953 - Dshi_0691 0.31 +0.4 696,962 + Dshi_0691 0.32 +2.4 696,988 + Dshi_0691 0.33 +0.8 696,998 - Dshi_0691 0.33 -0.4 696,998 - Dshi_0691 0.33 -1.9 697,116 - Dshi_0691 0.39 -0.9 697,216 + Dshi_0691 0.44 -1.6 697,321 - Dshi_0691 0.49 -1.3 697,377 - Dshi_0691 0.52 +0.1 697,417 + Dshi_0691 0.54 -0.2 697,440 + Dshi_0691 0.55 -1.0 697,448 - Dshi_0691 0.55 +2.4 697,448 - Dshi_0691 0.55 -2.3 697,476 + Dshi_0691 0.56 +2.8 697,500 + Dshi_0691 0.58 +0.4 697,608 + Dshi_0691 0.63 +4.0 697,635 - Dshi_0691 0.64 +0.2 697,663 + Dshi_0691 0.65 -0.8 697,707 + Dshi_0691 0.68 -1.1 697,707 + Dshi_0691 0.68 +0.6 697,721 - Dshi_0691 0.68 +1.3 697,785 - Dshi_0691 0.71 -0.6 697,851 - Dshi_0691 0.74 -0.6 697,946 + Dshi_0691 0.79 -2.4 697,977 + Dshi_0691 0.81 -2.0 698,072 + Dshi_0691 0.85 +1.0 698,074 + Dshi_0691 0.85 -2.9 698,080 - Dshi_0691 0.86 -1.0 698,144 - Dshi_0691 0.89 +0.2 698,237 + -1.0 698,422 + -0.3 698,489 + +0.6 698,757 - Dshi_0692 0.22 +0.1 698,825 + -0.6 698,928 + Dshi_0692 0.33 -0.3 698,936 - Dshi_0692 0.33 +0.4 698,936 - Dshi_0692 0.33 +1.5 698,949 + Dshi_0692 0.34 +0.2 698,975 + Dshi_0692 0.35 -0.5 699,101 + Dshi_0692 0.43 +0.4 699,107 - Dshi_0692 0.43 -0.2 699,117 - Dshi_0692 0.44 +0.1 699,195 + Dshi_0692 0.48 +1.0 699,213 - Dshi_0692 0.49 +0.2
Or see this region's nucleotide sequence