Experiment: m.b. Cholin acetate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0330 and Dshi_R0003 are separated by 171 nucleotides Dshi_R0003 and Dshi_0331 are separated by 6 nucleotides Dshi_0331 and Dshi_0332 overlap by 4 nucleotides Dshi_0332 and Dshi_0333 are separated by 62 nucleotides Dshi_0333 and Dshi_R0004 are separated by 155 nucleotides Dshi_R0004 and Dshi_0334 are separated by 51 nucleotides
Dshi_0330: Dshi_0330 - PP-loop domain protein (RefSeq), at 318,693 to 319,556
_0330
Dshi_R0003: Dshi_R0003 - tRNA-Thr (RefSeq), at 319,728 to 319,803
_R0003
Dshi_0331: Dshi_0331 - protein of unknown function DUF37 (RefSeq), at 319,810 to 320,031
_0331
Dshi_0332: Dshi_0332 - ribonuclease P protein component (RefSeq), at 320,028 to 320,417
_0332
Dshi_0333: Dshi_0333 - ribosomal protein L34 (RefSeq), at 320,480 to 320,614
_0333
Dshi_R0004: Dshi_R0004 - tRNA-Arg (RefSeq), at 320,770 to 320,846
_R0004
Dshi_0334: Dshi_0334 - integral membrane sensor signal transduction histidine kinase (RefSeq), at 320,898 to 322,301
_0334
Position (kb) 320
321 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 319.562 kb on - strand at 319.661 kb on + strand at 319.674 kb on - strand at 319.780 kb on - strand, within Dshi_R0003 at 319.872 kb on + strand, within Dshi_0331 at 319.953 kb on + strand, within Dshi_0331 at 319.983 kb on + strand, within Dshi_0331 at 321.235 kb on + strand, within Dshi_0334 at 321.243 kb on - strand, within Dshi_0334 at 321.379 kb on - strand, within Dshi_0334 at 321.490 kb on - strand, within Dshi_0334 at 321.563 kb on + strand, within Dshi_0334 at 321.563 kb on + strand, within Dshi_0334 at 321.578 kb on + strand, within Dshi_0334
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 40 mM remove 319,562 - -1.1 319,661 + +1.2 319,674 - +0.1 319,780 - Dshi_R0003 0.68 -2.9 319,872 + Dshi_0331 0.28 -2.5 319,953 + Dshi_0331 0.64 -1.7 319,983 + Dshi_0331 0.78 +2.4 321,235 + Dshi_0334 0.24 -0.7 321,243 - Dshi_0334 0.25 -0.3 321,379 - Dshi_0334 0.34 -0.1 321,490 - Dshi_0334 0.42 -2.9 321,563 + Dshi_0334 0.47 -3.3 321,563 + Dshi_0334 0.47 -0.8 321,578 + Dshi_0334 0.48 +1.1
Or see this region's nucleotide sequence