Experiment: m.b. Cholin acetate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0201 and Dshi_0202 are separated by 3 nucleotides Dshi_0202 and Dshi_0203 are separated by 68 nucleotides Dshi_0203 and Dshi_0204 are separated by 61 nucleotides
Dshi_0201: Dshi_0201 - GTP-binding protein Era (RefSeq), at 199,783 to 200,709
_0201
Dshi_0202: Dshi_0202 - protein of unknown function DUF1491 (RefSeq), at 200,713 to 201,045
_0202
Dshi_0203: Dshi_0203 - DNA repair protein RecO (RefSeq), at 201,114 to 201,839
_0203
Dshi_0204: Dshi_0204 - acyl-CoA dehydrogenase domain protein (RefSeq), at 201,901 to 203,595
_0204
Position (kb)
201
202 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 200.654 kb on + strand at 200.786 kb on + strand, within Dshi_0202 at 200.786 kb on + strand, within Dshi_0202 at 200.992 kb on + strand, within Dshi_0202 at 201.059 kb on - strand at 201.132 kb on - strand at 201.194 kb on - strand, within Dshi_0203 at 201.715 kb on + strand, within Dshi_0203 at 201.875 kb on + strand at 202.055 kb on + strand at 202.055 kb on + strand at 202.123 kb on - strand, within Dshi_0204 at 202.301 kb on - strand, within Dshi_0204 at 202.312 kb on - strand, within Dshi_0204 at 202.382 kb on + strand, within Dshi_0204 at 202.467 kb on - strand, within Dshi_0204 at 202.527 kb on + strand, within Dshi_0204 at 202.550 kb on + strand, within Dshi_0204 at 202.577 kb on - strand, within Dshi_0204 at 202.590 kb on - strand, within Dshi_0204 at 202.642 kb on + strand, within Dshi_0204 at 202.657 kb on + strand, within Dshi_0204 at 202.685 kb on + strand, within Dshi_0204
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 40 mM remove 200,654 + -0.1 200,786 + Dshi_0202 0.22 -4.0 200,786 + Dshi_0202 0.22 -1.4 200,992 + Dshi_0202 0.84 +0.2 201,059 - +0.4 201,132 - +0.9 201,194 - Dshi_0203 0.11 -0.1 201,715 + Dshi_0203 0.83 -1.8 201,875 + -1.7 202,055 + +3.1 202,055 + +3.5 202,123 - Dshi_0204 0.13 +3.0 202,301 - Dshi_0204 0.24 +2.0 202,312 - Dshi_0204 0.24 +4.5 202,382 + Dshi_0204 0.28 +3.1 202,467 - Dshi_0204 0.33 +2.1 202,527 + Dshi_0204 0.37 +2.9 202,550 + Dshi_0204 0.38 +1.8 202,577 - Dshi_0204 0.40 +2.0 202,590 - Dshi_0204 0.41 +3.3 202,642 + Dshi_0204 0.44 +3.3 202,657 + Dshi_0204 0.45 +3.4 202,685 + Dshi_0204 0.46 +2.8
Or see this region's nucleotide sequence