Experiment: m.b. Cholin acetate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0061 and Dshi_0062 are separated by 61 nucleotides Dshi_0062 and Dshi_0063 are separated by 122 nucleotides Dshi_0063 and Dshi_0064 overlap by 14 nucleotides
Dshi_0061: Dshi_0061 - Amidase (RefSeq), at 60,992 to 62,323
_0061
Dshi_0062: Dshi_0062 - Ubiquinone biosynthesis hydroxylase, UbiH/UbiF/VisC/COQ6 family (RefSeq), at 62,385 to 63,650
_0062
Dshi_0063: Dshi_0063 - Inositol-phosphate phosphatase (RefSeq), at 63,773 to 64,567
_0063
Dshi_0064: Dshi_0064 - peptidase U62 modulator of DNA gyrase (RefSeq), at 64,554 to 65,900
_0064
Position (kb) 62
63
64 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 61.453 kb on + strand, within Dshi_0061 at 61.488 kb on + strand, within Dshi_0061 at 61.652 kb on + strand, within Dshi_0061 at 61.654 kb on + strand, within Dshi_0061 at 61.748 kb on - strand, within Dshi_0061 at 61.775 kb on - strand, within Dshi_0061 at 61.822 kb on + strand, within Dshi_0061 at 61.860 kb on + strand, within Dshi_0061 at 61.920 kb on - strand, within Dshi_0061 at 62.077 kb on + strand, within Dshi_0061 at 62.164 kb on + strand, within Dshi_0061 at 62.254 kb on + strand at 62.292 kb on + strand at 62.395 kb on + strand at 62.419 kb on - strand at 63.616 kb on - strand at 63.645 kb on + strand at 63.645 kb on + strand at 63.653 kb on - strand at 63.698 kb on - strand at 63.711 kb on - strand at 63.857 kb on - strand, within Dshi_0063 at 63.857 kb on - strand, within Dshi_0063 at 63.891 kb on - strand, within Dshi_0063 at 64.002 kb on - strand, within Dshi_0063 at 64.157 kb on + strand, within Dshi_0063 at 64.188 kb on + strand, within Dshi_0063 at 64.259 kb on + strand, within Dshi_0063 at 64.264 kb on + strand, within Dshi_0063 at 64.479 kb on + strand, within Dshi_0063 at 64.569 kb on + strand at 64.606 kb on - strand at 64.613 kb on - strand at 64.641 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 40 mM remove 61,453 + Dshi_0061 0.35 -1.2 61,488 + Dshi_0061 0.37 -0.3 61,652 + Dshi_0061 0.50 +0.4 61,654 + Dshi_0061 0.50 +1.9 61,748 - Dshi_0061 0.57 +0.2 61,775 - Dshi_0061 0.59 +3.8 61,822 + Dshi_0061 0.62 +2.6 61,860 + Dshi_0061 0.65 -0.1 61,920 - Dshi_0061 0.70 -1.2 62,077 + Dshi_0061 0.81 +0.5 62,164 + Dshi_0061 0.88 +0.2 62,254 + +0.8 62,292 + -0.8 62,395 + +0.5 62,419 - -1.8 63,616 - +1.2 63,645 + -1.5 63,645 + -2.8 63,653 - +0.4 63,698 - -1.0 63,711 - +0.6 63,857 - Dshi_0063 0.11 +2.7 63,857 - Dshi_0063 0.11 +1.2 63,891 - Dshi_0063 0.15 +1.2 64,002 - Dshi_0063 0.29 +0.9 64,157 + Dshi_0063 0.48 +0.9 64,188 + Dshi_0063 0.52 -1.1 64,259 + Dshi_0063 0.61 -0.5 64,264 + Dshi_0063 0.62 +0.7 64,479 + Dshi_0063 0.89 -0.3 64,569 + +1.8 64,606 - +1.9 64,613 - +2.9 64,641 + +1.3
Or see this region's nucleotide sequence