Experiment: m.b. Cholin acetate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0039 and Dshi_0040 are separated by 27 nucleotides Dshi_0040 and Dshi_0041 are separated by 6 nucleotides Dshi_0041 and Dshi_0042 overlap by 4 nucleotides Dshi_0042 and Dshi_0043 are separated by 86 nucleotides
Dshi_0039: Dshi_0039 - GTP cyclohydrolase II (RefSeq), at 41,277 to 42,347
_0039
Dshi_0040: Dshi_0040 - protein of unknown function DUF1330 (RefSeq), at 42,375 to 42,662
_0040
Dshi_0041: Dshi_0041 - hypothetical protein (RefSeq), at 42,669 to 43,163
_0041
Dshi_0042: Dshi_0042 - alanine racemase domain protein (RefSeq), at 43,160 to 43,816
_0042
Dshi_0043: Dshi_0043 - hypothetical protein (RefSeq), at 43,903 to 44,862
_0043
Position (kb) 42
43
44 Strain fitness (log2 ratio) -2
-1
0
1
2 at 42.593 kb on + strand, within Dshi_0040 at 42.644 kb on - strand at 42.697 kb on + strand at 42.718 kb on + strand at 42.731 kb on - strand, within Dshi_0041 at 42.736 kb on - strand, within Dshi_0041 at 42.779 kb on - strand, within Dshi_0041 at 42.903 kb on + strand, within Dshi_0041 at 42.916 kb on + strand, within Dshi_0041 at 43.007 kb on - strand, within Dshi_0041 at 43.107 kb on + strand, within Dshi_0041 at 43.168 kb on + strand at 43.333 kb on + strand, within Dshi_0042 at 43.366 kb on + strand, within Dshi_0042 at 43.470 kb on - strand, within Dshi_0042 at 43.501 kb on + strand, within Dshi_0042 at 43.698 kb on + strand, within Dshi_0042 at 43.782 kb on + strand at 43.782 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 40 mM remove 42,593 + Dshi_0040 0.76 +0.5 42,644 - -0.8 42,697 + -1.7 42,718 + -0.3 42,731 - Dshi_0041 0.13 -0.3 42,736 - Dshi_0041 0.14 +0.2 42,779 - Dshi_0041 0.22 -1.2 42,903 + Dshi_0041 0.47 +1.9 42,916 + Dshi_0041 0.50 -0.8 43,007 - Dshi_0041 0.68 +1.5 43,107 + Dshi_0041 0.88 +1.2 43,168 + +0.6 43,333 + Dshi_0042 0.26 +0.7 43,366 + Dshi_0042 0.31 +2.0 43,470 - Dshi_0042 0.47 -0.8 43,501 + Dshi_0042 0.52 -1.8 43,698 + Dshi_0042 0.82 -1.6 43,782 + -0.2 43,782 + -0.1
Or see this region's nucleotide sequence