Experiment: m.b. acetate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2275 and Dshi_R0042 are separated by 81 nucleotides Dshi_R0042 and Dshi_2276 are separated by 300 nucleotides Dshi_2276 and Dshi_2277 overlap by 4 nucleotides
Dshi_2275: Dshi_2275 - single-stranded-DNA-specific exonuclease RecJ (RefSeq), at 2,398,150 to 2,399,907
_2275
Dshi_R0042: Dshi_R0042 - tRNA-Glu (RefSeq), at 2,399,989 to 2,400,063
_R0042
Dshi_2276: Dshi_2276 - hypothetical protein (RefSeq), at 2,400,364 to 2,400,894
_2276
Dshi_2277: Dshi_2277 - molybdenum cofactor synthesis domain (RefSeq), at 2,400,891 to 2,402,162
_2277
Position (kb)
2399
2400
2401 Strain fitness (log2 ratio)
-1
0
1 at 2399.893 kb on - strand at 2400.145 kb on - strand at 2400.223 kb on + strand at 2400.273 kb on - strand at 2400.335 kb on + strand at 2400.382 kb on + strand at 2400.414 kb on - strand at 2400.425 kb on - strand, within Dshi_2276 at 2400.504 kb on - strand, within Dshi_2276 at 2400.506 kb on + strand, within Dshi_2276 at 2400.509 kb on - strand, within Dshi_2276 at 2400.514 kb on - strand, within Dshi_2276 at 2400.514 kb on - strand, within Dshi_2276 at 2400.584 kb on + strand, within Dshi_2276 at 2400.592 kb on - strand, within Dshi_2276 at 2400.602 kb on - strand, within Dshi_2276 at 2400.645 kb on + strand, within Dshi_2276 at 2400.647 kb on + strand, within Dshi_2276 at 2400.647 kb on + strand, within Dshi_2276 at 2400.647 kb on + strand, within Dshi_2276 at 2400.663 kb on - strand, within Dshi_2276 at 2400.668 kb on - strand, within Dshi_2276 at 2400.669 kb on + strand, within Dshi_2276 at 2400.724 kb on - strand, within Dshi_2276 at 2400.728 kb on + strand, within Dshi_2276 at 2400.732 kb on + strand, within Dshi_2276 at 2400.732 kb on + strand, within Dshi_2276 at 2400.733 kb on + strand, within Dshi_2276 at 2400.740 kb on - strand, within Dshi_2276 at 2400.750 kb on + strand, within Dshi_2276 at 2400.778 kb on - strand, within Dshi_2276 at 2400.783 kb on - strand, within Dshi_2276 at 2400.783 kb on - strand, within Dshi_2276 at 2400.795 kb on + strand, within Dshi_2276 at 2400.810 kb on + strand, within Dshi_2276 at 2400.810 kb on + strand, within Dshi_2276 at 2400.810 kb on + strand, within Dshi_2276 at 2400.818 kb on - strand, within Dshi_2276 at 2400.920 kb on - strand at 2400.927 kb on + strand at 2400.927 kb on + strand at 2400.927 kb on + strand at 2400.950 kb on + strand at 2400.950 kb on + strand at 2400.951 kb on - strand at 2401.040 kb on + strand, within Dshi_2277 at 2401.040 kb on + strand, within Dshi_2277
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. acetate 200 mM remove 2,399,893 - +0.8 2,400,145 - +0.4 2,400,223 + -0.5 2,400,273 - -0.2 2,400,335 + +0.4 2,400,382 + -1.0 2,400,414 - +0.6 2,400,425 - Dshi_2276 0.11 -0.3 2,400,504 - Dshi_2276 0.26 -0.2 2,400,506 + Dshi_2276 0.27 -1.6 2,400,509 - Dshi_2276 0.27 +0.9 2,400,514 - Dshi_2276 0.28 -0.2 2,400,514 - Dshi_2276 0.28 -1.7 2,400,584 + Dshi_2276 0.41 +0.1 2,400,592 - Dshi_2276 0.43 +0.4 2,400,602 - Dshi_2276 0.45 -0.3 2,400,645 + Dshi_2276 0.53 +0.2 2,400,647 + Dshi_2276 0.53 +0.4 2,400,647 + Dshi_2276 0.53 -0.1 2,400,647 + Dshi_2276 0.53 -1.0 2,400,663 - Dshi_2276 0.56 -0.1 2,400,668 - Dshi_2276 0.57 -0.5 2,400,669 + Dshi_2276 0.57 +0.1 2,400,724 - Dshi_2276 0.68 -0.3 2,400,728 + Dshi_2276 0.69 -0.2 2,400,732 + Dshi_2276 0.69 +0.1 2,400,732 + Dshi_2276 0.69 +0.5 2,400,733 + Dshi_2276 0.69 +0.1 2,400,740 - Dshi_2276 0.71 -0.7 2,400,750 + Dshi_2276 0.73 -0.8 2,400,778 - Dshi_2276 0.78 +1.0 2,400,783 - Dshi_2276 0.79 +0.5 2,400,783 - Dshi_2276 0.79 -1.2 2,400,795 + Dshi_2276 0.81 -0.8 2,400,810 + Dshi_2276 0.84 -0.9 2,400,810 + Dshi_2276 0.84 -1.4 2,400,810 + Dshi_2276 0.84 +0.3 2,400,818 - Dshi_2276 0.85 +0.2 2,400,920 - +0.5 2,400,927 + -0.4 2,400,927 + -0.4 2,400,927 + -0.4 2,400,950 + +0.2 2,400,950 + +0.4 2,400,951 - +0.7 2,401,040 + Dshi_2277 0.12 -0.1 2,401,040 + Dshi_2277 0.12 +0.4
Or see this region's nucleotide sequence