Experiment: m.b. acetate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0123 and Dshi_0124 are separated by 145 nucleotides Dshi_0124 and Dshi_0125 are separated by 38 nucleotides Dshi_0125 and Dshi_0126 are separated by 103 nucleotides Dshi_0126 and Dshi_0127 overlap by 4 nucleotides
Dshi_0123: Dshi_0123 - transcriptional regulator, LysR family (RefSeq), at 121,186 to 122,094
_0123
Dshi_0124: Dshi_0124 - intracellular protease, PfpI family (RefSeq), at 122,240 to 122,824
_0124
Dshi_0125: Dshi_0125 - NADH:flavin oxidoreductase/NADH oxidase (RefSeq), at 122,863 to 124,881
_0125
Dshi_0126: Dshi_0126 - hypothetical protein (RefSeq), at 124,985 to 125,401
_0126
Dshi_0127: Dshi_0127 - hypothetical protein (RefSeq), at 125,398 to 126,168
_0127
Position (kb)
122
123
124
125 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 121.915 kb on - strand, within Dshi_0123 at 121.974 kb on + strand, within Dshi_0123 at 122.049 kb on + strand at 122.058 kb on + strand at 122.102 kb on + strand at 122.103 kb on + strand at 122.103 kb on + strand at 122.109 kb on - strand at 122.112 kb on + strand at 122.124 kb on + strand at 122.124 kb on + strand at 122.124 kb on + strand at 122.130 kb on - strand at 122.132 kb on - strand at 122.132 kb on - strand at 122.132 kb on - strand at 122.146 kb on + strand at 122.156 kb on + strand at 122.243 kb on + strand at 122.248 kb on + strand at 122.335 kb on + strand, within Dshi_0124 at 122.343 kb on - strand, within Dshi_0124 at 122.369 kb on - strand, within Dshi_0124 at 122.392 kb on - strand, within Dshi_0124 at 122.392 kb on - strand, within Dshi_0124 at 122.484 kb on + strand, within Dshi_0124 at 122.510 kb on + strand, within Dshi_0124 at 122.562 kb on + strand, within Dshi_0124 at 122.572 kb on + strand, within Dshi_0124 at 122.580 kb on - strand, within Dshi_0124 at 122.635 kb on + strand, within Dshi_0124 at 122.721 kb on + strand, within Dshi_0124 at 122.745 kb on - strand, within Dshi_0124 at 122.819 kb on - strand at 122.829 kb on - strand at 122.856 kb on + strand at 122.888 kb on + strand at 122.936 kb on - strand at 123.011 kb on - strand at 123.013 kb on + strand at 123.015 kb on + strand at 123.021 kb on - strand at 123.072 kb on + strand, within Dshi_0125 at 123.232 kb on - strand, within Dshi_0125 at 123.330 kb on - strand, within Dshi_0125 at 123.423 kb on - strand, within Dshi_0125 at 123.592 kb on + strand, within Dshi_0125 at 123.600 kb on - strand, within Dshi_0125 at 123.646 kb on - strand, within Dshi_0125 at 123.782 kb on - strand, within Dshi_0125 at 123.819 kb on + strand, within Dshi_0125 at 123.832 kb on - strand, within Dshi_0125 at 123.865 kb on + strand, within Dshi_0125 at 123.867 kb on - strand, within Dshi_0125 at 123.906 kb on - strand, within Dshi_0125 at 123.925 kb on + strand, within Dshi_0125 at 124.106 kb on + strand, within Dshi_0125 at 124.200 kb on + strand, within Dshi_0125 at 124.314 kb on - strand, within Dshi_0125 at 124.409 kb on - strand, within Dshi_0125 at 124.449 kb on + strand, within Dshi_0125 at 124.556 kb on + strand, within Dshi_0125 at 124.645 kb on - strand, within Dshi_0125 at 124.660 kb on - strand, within Dshi_0125 at 124.756 kb on - strand at 124.920 kb on + strand at 124.928 kb on - strand at 124.936 kb on - strand at 124.952 kb on - strand at 125.062 kb on - strand, within Dshi_0126 at 125.095 kb on + strand, within Dshi_0126 at 125.125 kb on - strand, within Dshi_0126 at 125.198 kb on + strand, within Dshi_0126 at 125.283 kb on - strand, within Dshi_0126 at 125.761 kb on - strand, within Dshi_0127
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. acetate 200 mM remove 121,915 - Dshi_0123 0.80 -2.0 121,974 + Dshi_0123 0.87 -0.2 122,049 + -0.6 122,058 + +0.1 122,102 + +0.1 122,103 + +0.1 122,103 + -1.0 122,109 - +0.0 122,112 + -0.9 122,124 + -1.0 122,124 + -1.2 122,124 + +1.6 122,130 - +0.6 122,132 - +0.2 122,132 - -1.0 122,132 - -0.0 122,146 + -1.2 122,156 + -0.3 122,243 + -0.0 122,248 + -2.1 122,335 + Dshi_0124 0.16 +0.9 122,343 - Dshi_0124 0.18 -1.3 122,369 - Dshi_0124 0.22 -1.9 122,392 - Dshi_0124 0.26 +0.9 122,392 - Dshi_0124 0.26 -1.6 122,484 + Dshi_0124 0.42 +1.3 122,510 + Dshi_0124 0.46 -1.3 122,562 + Dshi_0124 0.55 +0.2 122,572 + Dshi_0124 0.57 +0.3 122,580 - Dshi_0124 0.58 -0.4 122,635 + Dshi_0124 0.68 +0.3 122,721 + Dshi_0124 0.82 +0.2 122,745 - Dshi_0124 0.86 -0.2 122,819 - -0.7 122,829 - -0.8 122,856 + +0.2 122,888 + -1.1 122,936 - -0.4 123,011 - +0.2 123,013 + +2.4 123,015 + +2.4 123,021 - -0.5 123,072 + Dshi_0125 0.10 +1.0 123,232 - Dshi_0125 0.18 -0.7 123,330 - Dshi_0125 0.23 -1.0 123,423 - Dshi_0125 0.28 +0.1 123,592 + Dshi_0125 0.36 -1.4 123,600 - Dshi_0125 0.37 -1.6 123,646 - Dshi_0125 0.39 -1.6 123,782 - Dshi_0125 0.46 -0.4 123,819 + Dshi_0125 0.47 -1.5 123,832 - Dshi_0125 0.48 -0.9 123,865 + Dshi_0125 0.50 -0.1 123,867 - Dshi_0125 0.50 -0.6 123,906 - Dshi_0125 0.52 -2.1 123,925 + Dshi_0125 0.53 +0.6 124,106 + Dshi_0125 0.62 -0.8 124,200 + Dshi_0125 0.66 -2.8 124,314 - Dshi_0125 0.72 +0.1 124,409 - Dshi_0125 0.77 -0.8 124,449 + Dshi_0125 0.79 +0.0 124,556 + Dshi_0125 0.84 +0.1 124,645 - Dshi_0125 0.88 -0.2 124,660 - Dshi_0125 0.89 +1.0 124,756 - -0.4 124,920 + -1.3 124,928 - -0.5 124,936 - -0.6 124,952 - +2.0 125,062 - Dshi_0126 0.18 +0.4 125,095 + Dshi_0126 0.26 -1.3 125,125 - Dshi_0126 0.34 -1.3 125,198 + Dshi_0126 0.51 -0.1 125,283 - Dshi_0126 0.71 +0.2 125,761 - Dshi_0127 0.47 +3.4
Or see this region's nucleotide sequence