Experiment: m.b. Cholin acetate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3563 and Dshi_3564 are separated by 342 nucleotides Dshi_3564 and Dshi_3565 overlap by 4 nucleotides Dshi_3565 and Dshi_3566 are separated by 77 nucleotides
Dshi_3563: Dshi_3563 - translation initiation factor IF-2 (RefSeq), at 3,748,757 to 3,751,255
_3563
Dshi_3564: Dshi_3564 - mutator MutT protein (RefSeq), at 3,751,598 to 3,751,996
_3564
Dshi_3565: Dshi_3565 - arginine biosynthesis bifunctional protein ArgJ (RefSeq), at 3,751,993 to 3,753,216
_3565
Dshi_3566: Dshi_3566 - PpiC-type peptidyl-prolyl cis-trans isomerase (RefSeq), at 3,753,294 to 3,754,136
_3566
Position (kb)
3751
3752
3753
3754 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3751.253 kb on - strand at 3751.257 kb on - strand at 3751.363 kb on - strand at 3751.371 kb on - strand at 3751.445 kb on + strand at 3751.468 kb on + strand at 3751.479 kb on - strand at 3751.491 kb on - strand at 3751.491 kb on - strand at 3751.568 kb on + strand at 3751.568 kb on + strand at 3751.569 kb on + strand at 3751.577 kb on - strand at 3751.605 kb on - strand at 3751.655 kb on + strand, within Dshi_3564 at 3751.770 kb on - strand, within Dshi_3564 at 3751.772 kb on - strand, within Dshi_3564 at 3751.873 kb on + strand, within Dshi_3564 at 3751.928 kb on - strand, within Dshi_3564 at 3751.928 kb on - strand, within Dshi_3564 at 3751.933 kb on + strand, within Dshi_3564 at 3751.969 kb on - strand at 3752.081 kb on - strand at 3752.133 kb on + strand, within Dshi_3565 at 3752.242 kb on - strand, within Dshi_3565 at 3752.287 kb on - strand, within Dshi_3565 at 3752.287 kb on - strand, within Dshi_3565 at 3752.310 kb on + strand, within Dshi_3565 at 3752.313 kb on - strand, within Dshi_3565 at 3752.521 kb on - strand, within Dshi_3565 at 3752.627 kb on - strand, within Dshi_3565 at 3753.041 kb on - strand, within Dshi_3565 at 3753.049 kb on + strand, within Dshi_3565 at 3753.231 kb on + strand at 3753.256 kb on + strand at 3753.267 kb on + strand at 3753.305 kb on - strand at 3753.318 kb on + strand at 3753.386 kb on - strand, within Dshi_3566 at 3753.386 kb on - strand, within Dshi_3566 at 3753.387 kb on + strand, within Dshi_3566 at 3753.426 kb on + strand, within Dshi_3566 at 3753.435 kb on - strand, within Dshi_3566 at 3753.510 kb on - strand, within Dshi_3566 at 3753.543 kb on - strand, within Dshi_3566 at 3753.682 kb on + strand, within Dshi_3566 at 3753.718 kb on - strand, within Dshi_3566 at 3753.763 kb on + strand, within Dshi_3566 at 3753.838 kb on + strand, within Dshi_3566 at 3753.869 kb on + strand, within Dshi_3566 at 3753.897 kb on - strand, within Dshi_3566 at 3753.964 kb on + strand, within Dshi_3566 at 3753.973 kb on + strand, within Dshi_3566 at 3753.995 kb on - strand, within Dshi_3566 at 3753.995 kb on - strand, within Dshi_3566 at 3754.046 kb on - strand, within Dshi_3566 at 3754.085 kb on + strand at 3754.088 kb on + strand at 3754.110 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 40 mM remove 3,751,253 - -1.7 3,751,257 - +0.8 3,751,363 - -1.0 3,751,371 - -2.7 3,751,445 + -0.2 3,751,468 + -0.6 3,751,479 - +0.1 3,751,491 - +0.5 3,751,491 - -0.1 3,751,568 + +0.3 3,751,568 + +0.6 3,751,569 + +0.2 3,751,577 - +0.3 3,751,605 - -0.0 3,751,655 + Dshi_3564 0.14 -0.6 3,751,770 - Dshi_3564 0.43 +0.4 3,751,772 - Dshi_3564 0.44 +1.7 3,751,873 + Dshi_3564 0.69 -0.4 3,751,928 - Dshi_3564 0.83 -1.0 3,751,928 - Dshi_3564 0.83 -1.6 3,751,933 + Dshi_3564 0.84 +0.0 3,751,969 - +1.9 3,752,081 - -1.3 3,752,133 + Dshi_3565 0.11 -0.1 3,752,242 - Dshi_3565 0.20 -1.4 3,752,287 - Dshi_3565 0.24 -1.0 3,752,287 - Dshi_3565 0.24 -1.4 3,752,310 + Dshi_3565 0.26 -2.1 3,752,313 - Dshi_3565 0.26 -2.2 3,752,521 - Dshi_3565 0.43 -2.2 3,752,627 - Dshi_3565 0.52 -1.4 3,753,041 - Dshi_3565 0.86 -1.7 3,753,049 + Dshi_3565 0.86 -1.6 3,753,231 + -2.5 3,753,256 + -2.2 3,753,267 + +1.4 3,753,305 - +0.4 3,753,318 + -0.2 3,753,386 - Dshi_3566 0.11 -0.4 3,753,386 - Dshi_3566 0.11 -0.2 3,753,387 + Dshi_3566 0.11 -0.3 3,753,426 + Dshi_3566 0.16 -0.4 3,753,435 - Dshi_3566 0.17 +0.2 3,753,510 - Dshi_3566 0.26 -1.7 3,753,543 - Dshi_3566 0.30 +1.2 3,753,682 + Dshi_3566 0.46 -3.3 3,753,718 - Dshi_3566 0.50 -0.1 3,753,763 + Dshi_3566 0.56 -0.2 3,753,838 + Dshi_3566 0.65 -2.0 3,753,869 + Dshi_3566 0.68 -1.2 3,753,897 - Dshi_3566 0.72 -3.2 3,753,964 + Dshi_3566 0.79 +1.1 3,753,973 + Dshi_3566 0.81 -1.8 3,753,995 - Dshi_3566 0.83 +0.8 3,753,995 - Dshi_3566 0.83 -2.8 3,754,046 - Dshi_3566 0.89 -2.9 3,754,085 + -2.3 3,754,088 + -1.5 3,754,110 + +1.1
Or see this region's nucleotide sequence