Experiment: m.b. Cholin acetate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3556 and Dshi_3557 are separated by 124 nucleotides Dshi_3557 and Dshi_3558 are separated by 12 nucleotides Dshi_3558 and Dshi_3559 are separated by 20 nucleotides
Dshi_3556: Dshi_3556 - Na+/solute symporter (RefSeq), at 3,739,962 to 3,741,758
_3556
Dshi_3557: Dshi_3557 - putative CBS domain and cyclic nucleotide-regulated nucleotidyltransferase (RefSeq), at 3,741,883 to 3,743,700
_3557
Dshi_3558: Dshi_3558 - hypothetical protein (RefSeq), at 3,743,713 to 3,744,228
_3558
Dshi_3559: Dshi_3559 - DNA polymerase III, epsilon subunit (RefSeq), at 3,744,249 to 3,745,706
_3559
Position (kb)
3741
3742
3743
3744 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3740.926 kb on - strand, within Dshi_3556 at 3740.958 kb on + strand, within Dshi_3556 at 3740.999 kb on - strand, within Dshi_3556 at 3741.025 kb on + strand, within Dshi_3556 at 3741.046 kb on + strand, within Dshi_3556 at 3741.085 kb on + strand, within Dshi_3556 at 3741.253 kb on + strand, within Dshi_3556 at 3741.253 kb on + strand, within Dshi_3556 at 3741.411 kb on - strand, within Dshi_3556 at 3741.485 kb on - strand, within Dshi_3556 at 3741.496 kb on - strand, within Dshi_3556 at 3741.509 kb on - strand, within Dshi_3556 at 3741.517 kb on + strand, within Dshi_3556 at 3741.752 kb on + strand at 3741.930 kb on + strand at 3741.932 kb on + strand at 3741.991 kb on - strand at 3742.001 kb on - strand at 3742.047 kb on - strand at 3742.126 kb on - strand, within Dshi_3557 at 3742.273 kb on + strand, within Dshi_3557 at 3742.421 kb on - strand, within Dshi_3557 at 3742.620 kb on + strand, within Dshi_3557 at 3742.642 kb on + strand, within Dshi_3557 at 3742.738 kb on - strand, within Dshi_3557 at 3742.951 kb on + strand, within Dshi_3557 at 3743.133 kb on + strand, within Dshi_3557 at 3743.204 kb on - strand, within Dshi_3557 at 3743.220 kb on - strand, within Dshi_3557 at 3743.280 kb on - strand, within Dshi_3557 at 3743.298 kb on + strand, within Dshi_3557 at 3743.318 kb on - strand, within Dshi_3557 at 3743.487 kb on + strand, within Dshi_3557 at 3743.521 kb on - strand at 3743.521 kb on - strand at 3743.545 kb on + strand at 3743.593 kb on - strand at 3743.695 kb on + strand at 3743.702 kb on - strand at 3743.761 kb on + strand at 3743.789 kb on - strand, within Dshi_3558 at 3743.951 kb on - strand, within Dshi_3558 at 3743.995 kb on + strand, within Dshi_3558 at 3744.015 kb on - strand, within Dshi_3558 at 3744.109 kb on + strand, within Dshi_3558 at 3744.175 kb on + strand, within Dshi_3558 at 3744.185 kb on + strand at 3744.187 kb on + strand at 3744.187 kb on + strand at 3744.193 kb on - strand at 3744.207 kb on + strand at 3744.234 kb on + strand at 3744.265 kb on + strand at 3744.265 kb on + strand at 3744.326 kb on + strand at 3744.615 kb on + strand, within Dshi_3559 at 3744.644 kb on - strand, within Dshi_3559 at 3744.671 kb on + strand, within Dshi_3559 at 3744.681 kb on + strand, within Dshi_3559 at 3744.689 kb on - strand, within Dshi_3559 at 3744.695 kb on + strand, within Dshi_3559
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 40 mM remove 3,740,926 - Dshi_3556 0.54 -2.9 3,740,958 + Dshi_3556 0.55 +1.0 3,740,999 - Dshi_3556 0.58 -0.9 3,741,025 + Dshi_3556 0.59 +0.6 3,741,046 + Dshi_3556 0.60 -0.3 3,741,085 + Dshi_3556 0.62 -1.8 3,741,253 + Dshi_3556 0.72 -0.9 3,741,253 + Dshi_3556 0.72 -1.0 3,741,411 - Dshi_3556 0.81 -2.8 3,741,485 - Dshi_3556 0.85 -1.7 3,741,496 - Dshi_3556 0.85 -2.3 3,741,509 - Dshi_3556 0.86 -1.1 3,741,517 + Dshi_3556 0.87 -0.5 3,741,752 + -0.6 3,741,930 + -0.1 3,741,932 + +0.1 3,741,991 - -0.7 3,742,001 - +0.0 3,742,047 - -0.9 3,742,126 - Dshi_3557 0.13 -0.5 3,742,273 + Dshi_3557 0.21 -0.3 3,742,421 - Dshi_3557 0.30 +3.4 3,742,620 + Dshi_3557 0.41 -0.2 3,742,642 + Dshi_3557 0.42 -0.1 3,742,738 - Dshi_3557 0.47 +0.3 3,742,951 + Dshi_3557 0.59 +1.4 3,743,133 + Dshi_3557 0.69 +1.8 3,743,204 - Dshi_3557 0.73 +0.3 3,743,220 - Dshi_3557 0.74 +0.5 3,743,280 - Dshi_3557 0.77 -0.0 3,743,298 + Dshi_3557 0.78 +0.3 3,743,318 - Dshi_3557 0.79 -0.2 3,743,487 + Dshi_3557 0.88 +0.0 3,743,521 - +0.5 3,743,521 - +1.3 3,743,545 + -0.8 3,743,593 - +1.3 3,743,695 + -0.7 3,743,702 - +0.5 3,743,761 + -1.6 3,743,789 - Dshi_3558 0.15 -0.8 3,743,951 - Dshi_3558 0.46 -4.5 3,743,995 + Dshi_3558 0.55 -0.9 3,744,015 - Dshi_3558 0.59 -2.8 3,744,109 + Dshi_3558 0.77 -0.7 3,744,175 + Dshi_3558 0.90 -0.2 3,744,185 + -1.4 3,744,187 + -0.0 3,744,187 + -0.1 3,744,193 - -0.5 3,744,207 + +1.9 3,744,234 + -0.4 3,744,265 + -0.7 3,744,265 + +0.1 3,744,326 + +0.3 3,744,615 + Dshi_3559 0.25 -0.1 3,744,644 - Dshi_3559 0.27 +1.6 3,744,671 + Dshi_3559 0.29 -1.2 3,744,681 + Dshi_3559 0.30 +0.8 3,744,689 - Dshi_3559 0.30 -1.1 3,744,695 + Dshi_3559 0.31 -0.7
Or see this region's nucleotide sequence