Experiment: m.b. Cholin acetate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3226 and Dshi_3227 are separated by 19 nucleotides Dshi_3227 and Dshi_R0050 are separated by 136 nucleotides Dshi_R0050 and Dshi_3228 are separated by 96 nucleotides Dshi_3228 and Dshi_3229 overlap by 4 nucleotides
Dshi_3226: Dshi_3226 - hypothetical protein (RefSeq), at 3,395,713 to 3,395,988
_3226
Dshi_3227: Dshi_3227 - hypothetical protein (RefSeq), at 3,396,008 to 3,396,778
_3227
Dshi_R0050: Dshi_R0050 - tRNA-Lys (RefSeq), at 3,396,915 to 3,396,990
_R0050
Dshi_3228: Dshi_3228 - Diaminopimelate epimerase (RefSeq), at 3,397,087 to 3,397,935
_3228
Dshi_3229: Dshi_3229 - MiaB-like tRNA modifying enzyme (RefSeq), at 3,397,932 to 3,399,182
_3229
Position (kb)
3396
3397 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3395.977 kb on - strand at 3395.998 kb on - strand at 3396.038 kb on - strand at 3396.062 kb on - strand at 3396.131 kb on + strand, within Dshi_3227 at 3396.178 kb on + strand, within Dshi_3227 at 3396.282 kb on - strand, within Dshi_3227 at 3396.366 kb on + strand, within Dshi_3227 at 3396.457 kb on + strand, within Dshi_3227 at 3396.463 kb on + strand, within Dshi_3227 at 3396.491 kb on + strand, within Dshi_3227 at 3396.530 kb on + strand, within Dshi_3227 at 3396.542 kb on + strand, within Dshi_3227 at 3396.609 kb on - strand, within Dshi_3227 at 3396.692 kb on + strand, within Dshi_3227 at 3396.700 kb on - strand, within Dshi_3227 at 3396.703 kb on + strand at 3396.771 kb on - strand at 3397.075 kb on + strand at 3397.982 kb on + strand at 3397.982 kb on - strand at 3397.990 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 40 mM remove 3,395,977 - -2.8 3,395,998 - +2.2 3,396,038 - +0.6 3,396,062 - +0.0 3,396,131 + Dshi_3227 0.16 -0.2 3,396,178 + Dshi_3227 0.22 -0.8 3,396,282 - Dshi_3227 0.36 -0.8 3,396,366 + Dshi_3227 0.46 -0.2 3,396,457 + Dshi_3227 0.58 +0.9 3,396,463 + Dshi_3227 0.59 -1.6 3,396,491 + Dshi_3227 0.63 +0.9 3,396,530 + Dshi_3227 0.68 -2.3 3,396,542 + Dshi_3227 0.69 +1.0 3,396,609 - Dshi_3227 0.78 -0.1 3,396,692 + Dshi_3227 0.89 -1.1 3,396,700 - Dshi_3227 0.90 +0.2 3,396,703 + +1.4 3,396,771 - +2.0 3,397,075 + +0.9 3,397,982 + -0.6 3,397,982 - +0.1 3,397,990 - -0.2
Or see this region's nucleotide sequence