Experiment: m.b. Cholin acetate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3225 and Dshi_3226 are separated by 39 nucleotides Dshi_3226 and Dshi_3227 are separated by 19 nucleotides Dshi_3227 and Dshi_R0050 are separated by 136 nucleotides
Dshi_3225: Dshi_3225 - hypothetical protein (RefSeq), at 3,394,234 to 3,395,673
_3225
Dshi_3226: Dshi_3226 - hypothetical protein (RefSeq), at 3,395,713 to 3,395,988
_3226
Dshi_3227: Dshi_3227 - hypothetical protein (RefSeq), at 3,396,008 to 3,396,778
_3227
Dshi_R0050: Dshi_R0050 - tRNA-Lys (RefSeq), at 3,396,915 to 3,396,990
_R0050
Position (kb)
3395
3396 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3394.721 kb on - strand, within Dshi_3225 at 3394.736 kb on - strand, within Dshi_3225 at 3394.908 kb on + strand, within Dshi_3225 at 3394.915 kb on - strand, within Dshi_3225 at 3394.915 kb on - strand, within Dshi_3225 at 3394.915 kb on - strand, within Dshi_3225 at 3394.951 kb on + strand, within Dshi_3225 at 3394.972 kb on - strand, within Dshi_3225 at 3395.054 kb on + strand, within Dshi_3225 at 3395.082 kb on - strand, within Dshi_3225 at 3395.156 kb on + strand, within Dshi_3225 at 3395.204 kb on + strand, within Dshi_3225 at 3395.208 kb on - strand, within Dshi_3225 at 3395.250 kb on - strand, within Dshi_3225 at 3395.374 kb on - strand, within Dshi_3225 at 3395.379 kb on - strand, within Dshi_3225 at 3395.389 kb on - strand, within Dshi_3225 at 3395.424 kb on - strand, within Dshi_3225 at 3395.519 kb on + strand, within Dshi_3225 at 3395.519 kb on + strand, within Dshi_3225 at 3395.527 kb on - strand, within Dshi_3225 at 3395.577 kb on + strand at 3395.731 kb on + strand at 3395.733 kb on + strand at 3395.749 kb on + strand, within Dshi_3226 at 3395.752 kb on - strand, within Dshi_3226 at 3395.776 kb on + strand, within Dshi_3226 at 3395.784 kb on + strand, within Dshi_3226 at 3395.793 kb on + strand, within Dshi_3226 at 3395.801 kb on - strand, within Dshi_3226 at 3395.853 kb on + strand, within Dshi_3226 at 3395.977 kb on - strand at 3395.998 kb on - strand at 3396.038 kb on - strand at 3396.062 kb on - strand at 3396.131 kb on + strand, within Dshi_3227 at 3396.178 kb on + strand, within Dshi_3227 at 3396.282 kb on - strand, within Dshi_3227 at 3396.366 kb on + strand, within Dshi_3227 at 3396.457 kb on + strand, within Dshi_3227 at 3396.463 kb on + strand, within Dshi_3227 at 3396.491 kb on + strand, within Dshi_3227 at 3396.530 kb on + strand, within Dshi_3227 at 3396.542 kb on + strand, within Dshi_3227 at 3396.609 kb on - strand, within Dshi_3227 at 3396.692 kb on + strand, within Dshi_3227 at 3396.700 kb on - strand, within Dshi_3227 at 3396.703 kb on + strand at 3396.771 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 40 mM remove 3,394,721 - Dshi_3225 0.34 -2.1 3,394,736 - Dshi_3225 0.35 -2.0 3,394,908 + Dshi_3225 0.47 +1.7 3,394,915 - Dshi_3225 0.47 -0.1 3,394,915 - Dshi_3225 0.47 -0.6 3,394,915 - Dshi_3225 0.47 +1.4 3,394,951 + Dshi_3225 0.50 -0.9 3,394,972 - Dshi_3225 0.51 +0.8 3,395,054 + Dshi_3225 0.57 -0.4 3,395,082 - Dshi_3225 0.59 +1.3 3,395,156 + Dshi_3225 0.64 -1.7 3,395,204 + Dshi_3225 0.67 -0.6 3,395,208 - Dshi_3225 0.68 -0.7 3,395,250 - Dshi_3225 0.71 -0.4 3,395,374 - Dshi_3225 0.79 +0.2 3,395,379 - Dshi_3225 0.80 -1.7 3,395,389 - Dshi_3225 0.80 -1.0 3,395,424 - Dshi_3225 0.83 -2.3 3,395,519 + Dshi_3225 0.89 -1.1 3,395,519 + Dshi_3225 0.89 -1.4 3,395,527 - Dshi_3225 0.90 -0.2 3,395,577 + -0.6 3,395,731 + +0.3 3,395,733 + +0.6 3,395,749 + Dshi_3226 0.13 +0.4 3,395,752 - Dshi_3226 0.14 -0.9 3,395,776 + Dshi_3226 0.23 +0.5 3,395,784 + Dshi_3226 0.26 -0.2 3,395,793 + Dshi_3226 0.29 -2.4 3,395,801 - Dshi_3226 0.32 +2.2 3,395,853 + Dshi_3226 0.51 +0.6 3,395,977 - -2.8 3,395,998 - +2.2 3,396,038 - +0.6 3,396,062 - +0.0 3,396,131 + Dshi_3227 0.16 -0.2 3,396,178 + Dshi_3227 0.22 -0.8 3,396,282 - Dshi_3227 0.36 -0.8 3,396,366 + Dshi_3227 0.46 -0.2 3,396,457 + Dshi_3227 0.58 +0.9 3,396,463 + Dshi_3227 0.59 -1.6 3,396,491 + Dshi_3227 0.63 +0.9 3,396,530 + Dshi_3227 0.68 -2.3 3,396,542 + Dshi_3227 0.69 +1.0 3,396,609 - Dshi_3227 0.78 -0.1 3,396,692 + Dshi_3227 0.89 -1.1 3,396,700 - Dshi_3227 0.90 +0.2 3,396,703 + +1.4 3,396,771 - +2.0
Or see this region's nucleotide sequence