Experiment: m.b. Cholin acetate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2577 and Dshi_2578 are separated by 260 nucleotides Dshi_2578 and Dshi_2579 are separated by 143 nucleotides Dshi_2579 and Dshi_2580 are separated by 165 nucleotides
Dshi_2577: Dshi_2577 - putative RNA methylase (RefSeq), at 2,742,482 to 2,743,606
_2577
Dshi_2578: Dshi_2578 - phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (RefSeq), at 2,743,867 to 2,744,589
_2578
Dshi_2579: Dshi_2579 - hypothetical protein (RefSeq), at 2,744,733 to 2,745,137
_2579
Dshi_2580: Dshi_2580 - ATP-dependent helicase HrpB (RefSeq), at 2,745,303 to 2,747,807
_2580
Position (kb)
2743
2744
2745 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2742.956 kb on - strand, within Dshi_2577 at 2743.133 kb on - strand, within Dshi_2577 at 2743.255 kb on + strand, within Dshi_2577 at 2743.311 kb on + strand, within Dshi_2577 at 2743.373 kb on - strand, within Dshi_2577 at 2743.447 kb on + strand, within Dshi_2577 at 2743.498 kb on + strand at 2743.521 kb on - strand at 2743.629 kb on + strand at 2743.632 kb on - strand at 2743.638 kb on - strand at 2743.690 kb on + strand at 2743.698 kb on - strand at 2743.741 kb on + strand at 2743.855 kb on + strand at 2744.681 kb on - strand at 2744.810 kb on + strand, within Dshi_2579 at 2744.825 kb on + strand, within Dshi_2579 at 2744.833 kb on - strand, within Dshi_2579 at 2744.833 kb on - strand, within Dshi_2579 at 2744.833 kb on - strand, within Dshi_2579 at 2744.942 kb on - strand, within Dshi_2579 at 2744.942 kb on - strand, within Dshi_2579 at 2744.945 kb on + strand, within Dshi_2579 at 2744.985 kb on - strand, within Dshi_2579 at 2745.115 kb on + strand at 2745.123 kb on - strand at 2745.123 kb on - strand at 2745.177 kb on + strand at 2745.308 kb on - strand at 2745.335 kb on - strand at 2745.384 kb on + strand at 2745.414 kb on - strand at 2745.515 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 40 mM remove 2,742,956 - Dshi_2577 0.42 -1.7 2,743,133 - Dshi_2577 0.58 -1.5 2,743,255 + Dshi_2577 0.69 -1.0 2,743,311 + Dshi_2577 0.74 -0.1 2,743,373 - Dshi_2577 0.79 +0.1 2,743,447 + Dshi_2577 0.86 +1.3 2,743,498 + -0.4 2,743,521 - +4.6 2,743,629 + +2.6 2,743,632 - +2.6 2,743,638 - +0.1 2,743,690 + -0.9 2,743,698 - +1.0 2,743,741 + -0.4 2,743,855 + +1.1 2,744,681 - +0.2 2,744,810 + Dshi_2579 0.19 +0.6 2,744,825 + Dshi_2579 0.23 +0.9 2,744,833 - Dshi_2579 0.25 -0.2 2,744,833 - Dshi_2579 0.25 +0.2 2,744,833 - Dshi_2579 0.25 -0.7 2,744,942 - Dshi_2579 0.52 +0.1 2,744,942 - Dshi_2579 0.52 -1.7 2,744,945 + Dshi_2579 0.52 -1.8 2,744,985 - Dshi_2579 0.62 -0.6 2,745,115 + +3.8 2,745,123 - -1.1 2,745,123 - +0.1 2,745,177 + +0.3 2,745,308 - -1.5 2,745,335 - +0.3 2,745,384 + +1.4 2,745,414 - -0.2 2,745,515 + +1.9
Or see this region's nucleotide sequence