Experiment: m.b. Cholin acetate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2227 and Dshi_2228 are separated by 72 nucleotides Dshi_2228 and Dshi_2229 overlap by 4 nucleotides Dshi_2229 and Dshi_2230 are separated by 45 nucleotides
Dshi_2227: Dshi_2227 - FAD dependent oxidoreductase (RefSeq), at 2,350,024 to 2,351,328
_2227
Dshi_2228: Dshi_2228 - DegT/DnrJ/EryC1/StrS aminotransferase (RefSeq), at 2,351,401 to 2,352,594
_2228
Dshi_2229: Dshi_2229 - transcriptional regulator, LysR family (RefSeq), at 2,352,591 to 2,353,535
_2229
Dshi_2230: Dshi_2230 - polyhydroxyalkonate synthesis repressor, PhaR (RefSeq), at 2,353,581 to 2,354,141
_2230
Position (kb)
2351
2352
2353 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2350.414 kb on + strand, within Dshi_2227 at 2350.419 kb on + strand, within Dshi_2227 at 2350.461 kb on + strand, within Dshi_2227 at 2350.518 kb on - strand, within Dshi_2227 at 2350.598 kb on + strand, within Dshi_2227 at 2350.598 kb on - strand, within Dshi_2227 at 2350.653 kb on - strand, within Dshi_2227 at 2350.771 kb on + strand, within Dshi_2227 at 2350.841 kb on + strand, within Dshi_2227 at 2350.869 kb on - strand, within Dshi_2227 at 2351.297 kb on + strand at 2351.496 kb on - strand at 2351.501 kb on + strand at 2351.565 kb on + strand, within Dshi_2228 at 2351.573 kb on - strand, within Dshi_2228 at 2351.674 kb on + strand, within Dshi_2228 at 2351.689 kb on - strand, within Dshi_2228 at 2351.827 kb on - strand, within Dshi_2228 at 2351.859 kb on + strand, within Dshi_2228 at 2351.877 kb on + strand, within Dshi_2228 at 2351.902 kb on + strand, within Dshi_2228 at 2351.910 kb on - strand, within Dshi_2228 at 2352.116 kb on - strand, within Dshi_2228 at 2352.125 kb on + strand, within Dshi_2228 at 2352.243 kb on + strand, within Dshi_2228 at 2352.243 kb on + strand, within Dshi_2228 at 2352.248 kb on + strand, within Dshi_2228 at 2352.278 kb on - strand, within Dshi_2228 at 2352.384 kb on - strand, within Dshi_2228 at 2352.451 kb on - strand, within Dshi_2228 at 2352.500 kb on + strand at 2352.536 kb on + strand at 2352.625 kb on - strand at 2352.792 kb on - strand, within Dshi_2229 at 2352.824 kb on - strand, within Dshi_2229 at 2353.331 kb on + strand, within Dshi_2229 at 2353.349 kb on + strand, within Dshi_2229 at 2353.357 kb on - strand, within Dshi_2229 at 2353.375 kb on - strand, within Dshi_2229 at 2353.524 kb on + strand at 2353.524 kb on + strand at 2353.533 kb on - strand at 2353.566 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 40 mM remove 2,350,414 + Dshi_2227 0.30 +0.3 2,350,419 + Dshi_2227 0.30 +0.6 2,350,461 + Dshi_2227 0.33 -2.0 2,350,518 - Dshi_2227 0.38 -0.9 2,350,598 + Dshi_2227 0.44 -0.3 2,350,598 - Dshi_2227 0.44 +0.7 2,350,653 - Dshi_2227 0.48 -1.0 2,350,771 + Dshi_2227 0.57 -1.8 2,350,841 + Dshi_2227 0.63 -1.8 2,350,869 - Dshi_2227 0.65 -2.7 2,351,297 + -0.9 2,351,496 - -0.3 2,351,501 + +0.3 2,351,565 + Dshi_2228 0.14 +1.1 2,351,573 - Dshi_2228 0.14 -0.8 2,351,674 + Dshi_2228 0.23 -0.1 2,351,689 - Dshi_2228 0.24 -2.0 2,351,827 - Dshi_2228 0.36 +1.3 2,351,859 + Dshi_2228 0.38 -2.2 2,351,877 + Dshi_2228 0.40 -2.6 2,351,902 + Dshi_2228 0.42 +0.1 2,351,910 - Dshi_2228 0.43 -1.4 2,352,116 - Dshi_2228 0.60 -1.2 2,352,125 + Dshi_2228 0.61 -0.2 2,352,243 + Dshi_2228 0.71 +0.4 2,352,243 + Dshi_2228 0.71 +0.2 2,352,248 + Dshi_2228 0.71 +0.0 2,352,278 - Dshi_2228 0.73 +0.7 2,352,384 - Dshi_2228 0.82 -2.4 2,352,451 - Dshi_2228 0.88 +1.8 2,352,500 + +1.0 2,352,536 + +0.2 2,352,625 - +0.2 2,352,792 - Dshi_2229 0.21 -0.5 2,352,824 - Dshi_2229 0.25 -0.6 2,353,331 + Dshi_2229 0.78 -1.2 2,353,349 + Dshi_2229 0.80 +1.4 2,353,357 - Dshi_2229 0.81 -0.9 2,353,375 - Dshi_2229 0.83 +0.3 2,353,524 + -0.2 2,353,524 + +1.2 2,353,533 - -1.4 2,353,566 + +0.5
Or see this region's nucleotide sequence