Experiment: m.b. Cholin acetate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2209 and Dshi_2210 are separated by 158 nucleotides Dshi_2210 and Dshi_2211 are separated by 102 nucleotides Dshi_2211 and Dshi_2212 are separated by 9 nucleotides
Dshi_2209: Dshi_2209 - protein of unknown function DUF6 transmembrane (RefSeq), at 2,335,419 to 2,336,249
_2209
Dshi_2210: Dshi_2210 - hypothetical protein (RefSeq), at 2,336,408 to 2,338,699
_2210
Dshi_2211: Dshi_2211 - protein of unknown function DUF427 (RefSeq), at 2,338,802 to 2,339,278
_2211
Dshi_2212: Dshi_2212 - pyridoxamine 5'-phosphate oxidase-related FMN-binding (RefSeq), at 2,339,288 to 2,339,782
_2212
Position (kb)
2336
2337
2338
2339 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2335.482 kb on + strand at 2335.534 kb on + strand, within Dshi_2209 at 2335.539 kb on + strand, within Dshi_2209 at 2335.547 kb on - strand, within Dshi_2209 at 2335.631 kb on + strand, within Dshi_2209 at 2335.641 kb on + strand, within Dshi_2209 at 2335.656 kb on - strand, within Dshi_2209 at 2335.672 kb on - strand, within Dshi_2209 at 2335.793 kb on - strand, within Dshi_2209 at 2335.837 kb on + strand, within Dshi_2209 at 2335.885 kb on + strand, within Dshi_2209 at 2336.006 kb on - strand, within Dshi_2209 at 2336.016 kb on - strand, within Dshi_2209 at 2336.049 kb on + strand, within Dshi_2209 at 2336.050 kb on - strand, within Dshi_2209 at 2336.150 kb on - strand, within Dshi_2209 at 2336.184 kb on + strand at 2336.187 kb on - strand at 2336.187 kb on - strand at 2336.207 kb on + strand at 2336.279 kb on + strand at 2336.292 kb on - strand at 2336.374 kb on + strand at 2336.471 kb on - strand at 2336.485 kb on - strand at 2336.531 kb on + strand at 2336.703 kb on - strand, within Dshi_2210 at 2336.706 kb on + strand, within Dshi_2210 at 2336.715 kb on - strand, within Dshi_2210 at 2336.761 kb on + strand, within Dshi_2210 at 2336.848 kb on - strand, within Dshi_2210 at 2336.943 kb on + strand, within Dshi_2210 at 2336.943 kb on + strand, within Dshi_2210 at 2336.951 kb on - strand, within Dshi_2210 at 2337.000 kb on + strand, within Dshi_2210 at 2337.198 kb on + strand, within Dshi_2210 at 2337.229 kb on - strand, within Dshi_2210 at 2337.283 kb on - strand, within Dshi_2210 at 2337.385 kb on + strand, within Dshi_2210 at 2337.385 kb on + strand, within Dshi_2210 at 2337.393 kb on - strand, within Dshi_2210 at 2337.417 kb on + strand, within Dshi_2210 at 2337.419 kb on + strand, within Dshi_2210 at 2337.424 kb on + strand, within Dshi_2210 at 2337.437 kb on - strand, within Dshi_2210 at 2337.507 kb on + strand, within Dshi_2210 at 2337.539 kb on + strand, within Dshi_2210 at 2337.579 kb on + strand, within Dshi_2210 at 2337.616 kb on + strand, within Dshi_2210 at 2337.616 kb on + strand, within Dshi_2210 at 2337.616 kb on + strand, within Dshi_2210 at 2337.647 kb on + strand, within Dshi_2210 at 2337.650 kb on + strand, within Dshi_2210 at 2337.727 kb on - strand, within Dshi_2210 at 2337.761 kb on + strand, within Dshi_2210 at 2337.923 kb on + strand, within Dshi_2210 at 2337.945 kb on - strand, within Dshi_2210 at 2337.960 kb on - strand, within Dshi_2210 at 2337.973 kb on + strand, within Dshi_2210 at 2337.979 kb on - strand, within Dshi_2210 at 2338.017 kb on - strand, within Dshi_2210 at 2338.065 kb on + strand, within Dshi_2210 at 2338.083 kb on + strand, within Dshi_2210 at 2338.091 kb on - strand, within Dshi_2210 at 2338.165 kb on + strand, within Dshi_2210 at 2338.168 kb on - strand, within Dshi_2210 at 2338.188 kb on - strand, within Dshi_2210 at 2338.220 kb on - strand, within Dshi_2210 at 2338.274 kb on + strand, within Dshi_2210 at 2338.327 kb on + strand, within Dshi_2210 at 2338.335 kb on - strand, within Dshi_2210 at 2338.361 kb on + strand, within Dshi_2210 at 2338.376 kb on + strand, within Dshi_2210 at 2338.386 kb on + strand, within Dshi_2210 at 2338.442 kb on - strand, within Dshi_2210 at 2338.456 kb on + strand, within Dshi_2210 at 2338.459 kb on - strand, within Dshi_2210 at 2338.641 kb on + strand at 2338.649 kb on - strand at 2338.664 kb on + strand at 2338.724 kb on - strand at 2338.755 kb on - strand at 2338.755 kb on - strand at 2338.788 kb on + strand at 2338.836 kb on + strand at 2338.860 kb on + strand, within Dshi_2211 at 2338.951 kb on + strand, within Dshi_2211 at 2338.953 kb on + strand, within Dshi_2211 at 2338.960 kb on + strand, within Dshi_2211 at 2338.991 kb on - strand, within Dshi_2211 at 2339.041 kb on + strand, within Dshi_2211 at 2339.136 kb on - strand, within Dshi_2211 at 2339.158 kb on - strand, within Dshi_2211 at 2339.182 kb on - strand at 2339.325 kb on + strand at 2339.361 kb on + strand, within Dshi_2212 at 2339.403 kb on + strand, within Dshi_2212 at 2339.407 kb on + strand, within Dshi_2212 at 2339.410 kb on - strand, within Dshi_2212 at 2339.432 kb on + strand, within Dshi_2212 at 2339.443 kb on + strand, within Dshi_2212 at 2339.451 kb on - strand, within Dshi_2212 at 2339.474 kb on + strand, within Dshi_2212 at 2339.570 kb on - strand, within Dshi_2212
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 40 mM remove 2,335,482 + -1.8 2,335,534 + Dshi_2209 0.14 +0.7 2,335,539 + Dshi_2209 0.14 +2.2 2,335,547 - Dshi_2209 0.15 +0.8 2,335,631 + Dshi_2209 0.26 -0.7 2,335,641 + Dshi_2209 0.27 -2.7 2,335,656 - Dshi_2209 0.29 +0.6 2,335,672 - Dshi_2209 0.30 -0.4 2,335,793 - Dshi_2209 0.45 +0.2 2,335,837 + Dshi_2209 0.50 -0.2 2,335,885 + Dshi_2209 0.56 -1.2 2,336,006 - Dshi_2209 0.71 -0.8 2,336,016 - Dshi_2209 0.72 -2.1 2,336,049 + Dshi_2209 0.76 +3.0 2,336,050 - Dshi_2209 0.76 -0.7 2,336,150 - Dshi_2209 0.88 +0.9 2,336,184 + +0.3 2,336,187 - -0.1 2,336,187 - -1.0 2,336,207 + +1.1 2,336,279 + -2.4 2,336,292 - +1.8 2,336,374 + -1.0 2,336,471 - +1.8 2,336,485 - -0.2 2,336,531 + -0.2 2,336,703 - Dshi_2210 0.13 -0.6 2,336,706 + Dshi_2210 0.13 -0.0 2,336,715 - Dshi_2210 0.13 +1.0 2,336,761 + Dshi_2210 0.15 +0.7 2,336,848 - Dshi_2210 0.19 -0.9 2,336,943 + Dshi_2210 0.23 +2.0 2,336,943 + Dshi_2210 0.23 +2.3 2,336,951 - Dshi_2210 0.24 +1.2 2,337,000 + Dshi_2210 0.26 +1.5 2,337,198 + Dshi_2210 0.34 -0.3 2,337,229 - Dshi_2210 0.36 -1.0 2,337,283 - Dshi_2210 0.38 -0.4 2,337,385 + Dshi_2210 0.43 +1.6 2,337,385 + Dshi_2210 0.43 +0.8 2,337,393 - Dshi_2210 0.43 +0.1 2,337,417 + Dshi_2210 0.44 +0.7 2,337,419 + Dshi_2210 0.44 +0.3 2,337,424 + Dshi_2210 0.44 +2.2 2,337,437 - Dshi_2210 0.45 +0.5 2,337,507 + Dshi_2210 0.48 -1.8 2,337,539 + Dshi_2210 0.49 -2.9 2,337,579 + Dshi_2210 0.51 -0.3 2,337,616 + Dshi_2210 0.53 +0.8 2,337,616 + Dshi_2210 0.53 -0.9 2,337,616 + Dshi_2210 0.53 +0.8 2,337,647 + Dshi_2210 0.54 -1.6 2,337,650 + Dshi_2210 0.54 +0.1 2,337,727 - Dshi_2210 0.58 -1.3 2,337,761 + Dshi_2210 0.59 +1.0 2,337,923 + Dshi_2210 0.66 -1.2 2,337,945 - Dshi_2210 0.67 +1.8 2,337,960 - Dshi_2210 0.68 +1.5 2,337,973 + Dshi_2210 0.68 +2.4 2,337,979 - Dshi_2210 0.69 -1.4 2,338,017 - Dshi_2210 0.70 +1.0 2,338,065 + Dshi_2210 0.72 +1.5 2,338,083 + Dshi_2210 0.73 -0.1 2,338,091 - Dshi_2210 0.73 -0.8 2,338,165 + Dshi_2210 0.77 +1.1 2,338,168 - Dshi_2210 0.77 -0.9 2,338,188 - Dshi_2210 0.78 -0.8 2,338,220 - Dshi_2210 0.79 -0.2 2,338,274 + Dshi_2210 0.81 -0.1 2,338,327 + Dshi_2210 0.84 +0.1 2,338,335 - Dshi_2210 0.84 -0.5 2,338,361 + Dshi_2210 0.85 +0.5 2,338,376 + Dshi_2210 0.86 -2.1 2,338,386 + Dshi_2210 0.86 -2.2 2,338,442 - Dshi_2210 0.89 +0.5 2,338,456 + Dshi_2210 0.89 +4.2 2,338,459 - Dshi_2210 0.89 +0.3 2,338,641 + +1.0 2,338,649 - +0.4 2,338,664 + -1.7 2,338,724 - -0.3 2,338,755 - +0.5 2,338,755 - +1.5 2,338,788 + -3.1 2,338,836 + -0.4 2,338,860 + Dshi_2211 0.12 -0.1 2,338,951 + Dshi_2211 0.31 +0.3 2,338,953 + Dshi_2211 0.32 -2.7 2,338,960 + Dshi_2211 0.33 -0.7 2,338,991 - Dshi_2211 0.40 -0.3 2,339,041 + Dshi_2211 0.50 +0.1 2,339,136 - Dshi_2211 0.70 -1.3 2,339,158 - Dshi_2211 0.75 -2.1 2,339,182 - +2.2 2,339,325 + -2.4 2,339,361 + Dshi_2212 0.15 -1.2 2,339,403 + Dshi_2212 0.23 +0.0 2,339,407 + Dshi_2212 0.24 +1.5 2,339,410 - Dshi_2212 0.25 -0.4 2,339,432 + Dshi_2212 0.29 -3.0 2,339,443 + Dshi_2212 0.31 -0.6 2,339,451 - Dshi_2212 0.33 +0.7 2,339,474 + Dshi_2212 0.38 +0.2 2,339,570 - Dshi_2212 0.57 -0.6
Or see this region's nucleotide sequence