Experiment: m.b. Cholin acetate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2174 and Dshi_2175 overlap by 4 nucleotides Dshi_2175 and Dshi_2176 are separated by 12 nucleotides Dshi_2176 and Dshi_2177 overlap by 10 nucleotides
Dshi_2174: Dshi_2174 - phage major capsid protein, HK97 family (RefSeq), at 2,301,398 to 2,302,615
_2174
Dshi_2175: Dshi_2175 - phage prohead protease, HK97 family (RefSeq), at 2,302,612 to 2,303,229
_2175
Dshi_2176: Dshi_2176 - hypothetical protein (RefSeq), at 2,303,242 to 2,303,508
_2176
Dshi_2177: Dshi_2177 - phage portal protein, HK97 family (RefSeq), at 2,303,499 to 2,304,689
_2177
Position (kb)
2302
2303
2304 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2301.619 kb on - strand, within Dshi_2174 at 2301.634 kb on - strand, within Dshi_2174 at 2301.698 kb on - strand, within Dshi_2174 at 2301.725 kb on - strand, within Dshi_2174 at 2301.768 kb on + strand, within Dshi_2174 at 2301.817 kb on - strand, within Dshi_2174 at 2301.850 kb on - strand, within Dshi_2174 at 2301.854 kb on - strand, within Dshi_2174 at 2301.896 kb on + strand, within Dshi_2174 at 2301.898 kb on - strand, within Dshi_2174 at 2302.000 kb on - strand, within Dshi_2174 at 2302.034 kb on - strand, within Dshi_2174 at 2302.034 kb on - strand, within Dshi_2174 at 2302.091 kb on - strand, within Dshi_2174 at 2302.153 kb on + strand, within Dshi_2174 at 2302.161 kb on - strand, within Dshi_2174 at 2302.233 kb on + strand, within Dshi_2174 at 2302.241 kb on + strand, within Dshi_2174 at 2302.314 kb on - strand, within Dshi_2174 at 2302.389 kb on + strand, within Dshi_2174 at 2302.434 kb on + strand, within Dshi_2174 at 2302.442 kb on - strand, within Dshi_2174 at 2302.442 kb on - strand, within Dshi_2174 at 2302.442 kb on - strand, within Dshi_2174 at 2302.484 kb on + strand, within Dshi_2174 at 2302.492 kb on - strand, within Dshi_2174 at 2302.492 kb on - strand, within Dshi_2174 at 2302.527 kb on - strand at 2302.599 kb on - strand at 2302.651 kb on - strand at 2302.670 kb on - strand at 2302.673 kb on + strand at 2302.777 kb on - strand, within Dshi_2175 at 2302.943 kb on + strand, within Dshi_2175 at 2302.981 kb on + strand, within Dshi_2175 at 2302.986 kb on + strand, within Dshi_2175 at 2303.106 kb on + strand, within Dshi_2175 at 2303.114 kb on - strand, within Dshi_2175 at 2303.118 kb on - strand, within Dshi_2175 at 2303.160 kb on - strand, within Dshi_2175 at 2303.195 kb on + strand at 2303.293 kb on - strand, within Dshi_2176 at 2303.439 kb on - strand, within Dshi_2176 at 2303.477 kb on + strand, within Dshi_2176 at 2303.498 kb on + strand at 2303.498 kb on + strand at 2303.549 kb on - strand at 2303.588 kb on - strand at 2303.618 kb on - strand, within Dshi_2177 at 2303.669 kb on + strand, within Dshi_2177 at 2303.706 kb on + strand, within Dshi_2177 at 2303.721 kb on - strand, within Dshi_2177 at 2303.831 kb on + strand, within Dshi_2177 at 2303.890 kb on + strand, within Dshi_2177 at 2303.973 kb on - strand, within Dshi_2177 at 2304.053 kb on - strand, within Dshi_2177 at 2304.064 kb on - strand, within Dshi_2177 at 2304.151 kb on - strand, within Dshi_2177 at 2304.206 kb on - strand, within Dshi_2177 at 2304.214 kb on + strand, within Dshi_2177 at 2304.221 kb on + strand, within Dshi_2177 at 2304.229 kb on - strand, within Dshi_2177
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 40 mM remove 2,301,619 - Dshi_2174 0.18 -0.1 2,301,634 - Dshi_2174 0.19 +1.4 2,301,698 - Dshi_2174 0.25 -2.1 2,301,725 - Dshi_2174 0.27 -1.4 2,301,768 + Dshi_2174 0.30 +1.0 2,301,817 - Dshi_2174 0.34 -0.6 2,301,850 - Dshi_2174 0.37 -1.0 2,301,854 - Dshi_2174 0.37 -1.5 2,301,896 + Dshi_2174 0.41 +0.5 2,301,898 - Dshi_2174 0.41 -0.9 2,302,000 - Dshi_2174 0.49 -0.4 2,302,034 - Dshi_2174 0.52 +0.1 2,302,034 - Dshi_2174 0.52 +1.8 2,302,091 - Dshi_2174 0.57 -1.9 2,302,153 + Dshi_2174 0.62 -1.5 2,302,161 - Dshi_2174 0.63 +2.9 2,302,233 + Dshi_2174 0.69 -0.5 2,302,241 + Dshi_2174 0.69 +1.0 2,302,314 - Dshi_2174 0.75 +0.3 2,302,389 + Dshi_2174 0.81 +0.2 2,302,434 + Dshi_2174 0.85 +0.4 2,302,442 - Dshi_2174 0.86 -1.0 2,302,442 - Dshi_2174 0.86 +0.8 2,302,442 - Dshi_2174 0.86 -0.2 2,302,484 + Dshi_2174 0.89 -0.7 2,302,492 - Dshi_2174 0.90 +0.7 2,302,492 - Dshi_2174 0.90 -1.4 2,302,527 - +0.0 2,302,599 - -0.8 2,302,651 - -0.3 2,302,670 - +0.9 2,302,673 + -0.8 2,302,777 - Dshi_2175 0.27 -0.3 2,302,943 + Dshi_2175 0.54 +0.7 2,302,981 + Dshi_2175 0.60 -2.4 2,302,986 + Dshi_2175 0.61 -1.2 2,303,106 + Dshi_2175 0.80 -2.3 2,303,114 - Dshi_2175 0.81 -0.8 2,303,118 - Dshi_2175 0.82 -1.9 2,303,160 - Dshi_2175 0.89 -0.3 2,303,195 + -2.4 2,303,293 - Dshi_2176 0.19 -0.5 2,303,439 - Dshi_2176 0.74 -0.8 2,303,477 + Dshi_2176 0.88 -0.1 2,303,498 + -1.7 2,303,498 + -1.6 2,303,549 - +0.1 2,303,588 - -3.1 2,303,618 - Dshi_2177 0.10 -0.2 2,303,669 + Dshi_2177 0.14 +0.5 2,303,706 + Dshi_2177 0.17 +0.0 2,303,721 - Dshi_2177 0.19 -0.2 2,303,831 + Dshi_2177 0.28 -0.9 2,303,890 + Dshi_2177 0.33 +0.1 2,303,973 - Dshi_2177 0.40 +1.3 2,304,053 - Dshi_2177 0.47 +0.7 2,304,064 - Dshi_2177 0.47 -0.5 2,304,151 - Dshi_2177 0.55 -0.6 2,304,206 - Dshi_2177 0.59 +0.3 2,304,214 + Dshi_2177 0.60 -0.4 2,304,221 + Dshi_2177 0.61 -0.5 2,304,229 - Dshi_2177 0.61 +1.4
Or see this region's nucleotide sequence