Experiment: m.b. Cholin acetate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1967 and Dshi_1968 are separated by 54 nucleotides Dshi_1968 and Dshi_5004 are separated by 228 nucleotides
Dshi_1967: Dshi_1967 - catalytic domain of components of various dehydrogenase complexes (RefSeq), at 2,075,886 to 2,077,187
_1967
Dshi_1968: Dshi_1968 - 2-oxo-acid dehydrogenase E1 subunit, homodimeric type (RefSeq), at 2,077,242 to 2,079,899
_1968
Dshi_5004: Dshi_5004 - D-lactate dehydrogenase (RefSeq), at 2,080,128 to 2,081,843
_5004
Position (kb)
2077
2078
2079
2080 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2076.281 kb on + strand, within Dshi_1967 at 2076.289 kb on - strand, within Dshi_1967 at 2076.363 kb on + strand, within Dshi_1967 at 2076.424 kb on + strand, within Dshi_1967 at 2076.424 kb on + strand, within Dshi_1967 at 2076.432 kb on - strand, within Dshi_1967 at 2076.432 kb on - strand, within Dshi_1967 at 2076.787 kb on - strand, within Dshi_1967 at 2076.791 kb on - strand, within Dshi_1967 at 2077.107 kb on - strand at 2077.150 kb on - strand at 2077.170 kb on - strand at 2077.216 kb on + strand at 2077.245 kb on + strand at 2077.449 kb on - strand at 2077.518 kb on + strand, within Dshi_1968 at 2077.766 kb on + strand, within Dshi_1968 at 2077.798 kb on - strand, within Dshi_1968 at 2077.809 kb on + strand, within Dshi_1968 at 2077.827 kb on - strand, within Dshi_1968 at 2077.870 kb on + strand, within Dshi_1968 at 2078.021 kb on + strand, within Dshi_1968 at 2078.036 kb on + strand, within Dshi_1968 at 2078.048 kb on + strand, within Dshi_1968 at 2078.076 kb on - strand, within Dshi_1968 at 2078.138 kb on - strand, within Dshi_1968 at 2078.205 kb on + strand, within Dshi_1968 at 2078.242 kb on - strand, within Dshi_1968 at 2078.463 kb on + strand, within Dshi_1968 at 2078.492 kb on + strand, within Dshi_1968 at 2078.577 kb on + strand, within Dshi_1968 at 2078.577 kb on + strand, within Dshi_1968 at 2078.577 kb on + strand, within Dshi_1968 at 2078.583 kb on - strand, within Dshi_1968 at 2078.585 kb on - strand, within Dshi_1968 at 2078.626 kb on + strand, within Dshi_1968 at 2078.742 kb on - strand, within Dshi_1968 at 2078.744 kb on + strand, within Dshi_1968 at 2078.815 kb on - strand, within Dshi_1968 at 2078.830 kb on + strand, within Dshi_1968 at 2078.830 kb on + strand, within Dshi_1968 at 2078.912 kb on - strand, within Dshi_1968 at 2079.013 kb on + strand, within Dshi_1968 at 2079.014 kb on + strand, within Dshi_1968 at 2079.022 kb on - strand, within Dshi_1968 at 2079.027 kb on - strand, within Dshi_1968 at 2079.027 kb on - strand, within Dshi_1968 at 2079.158 kb on - strand, within Dshi_1968 at 2079.225 kb on + strand, within Dshi_1968 at 2079.257 kb on + strand, within Dshi_1968 at 2079.265 kb on - strand, within Dshi_1968 at 2079.339 kb on - strand, within Dshi_1968 at 2079.390 kb on + strand, within Dshi_1968 at 2079.391 kb on - strand, within Dshi_1968 at 2079.478 kb on - strand, within Dshi_1968 at 2079.479 kb on - strand, within Dshi_1968 at 2079.498 kb on - strand, within Dshi_1968 at 2079.537 kb on + strand, within Dshi_1968 at 2079.620 kb on + strand, within Dshi_1968 at 2079.625 kb on + strand, within Dshi_1968 at 2079.626 kb on - strand, within Dshi_1968 at 2079.633 kb on - strand, within Dshi_1968 at 2079.633 kb on - strand, within Dshi_1968 at 2079.720 kb on + strand at 2079.755 kb on - strand at 2079.814 kb on + strand at 2079.915 kb on + strand at 2079.923 kb on + strand at 2079.923 kb on - strand at 2079.970 kb on - strand at 2080.001 kb on + strand at 2080.109 kb on - strand at 2080.228 kb on + strand at 2080.231 kb on + strand at 2080.236 kb on - strand at 2080.237 kb on + strand at 2080.352 kb on - strand, within Dshi_5004 at 2080.393 kb on + strand, within Dshi_5004 at 2080.396 kb on - strand, within Dshi_5004 at 2080.398 kb on - strand, within Dshi_5004 at 2080.487 kb on + strand, within Dshi_5004 at 2080.494 kb on - strand, within Dshi_5004 at 2080.510 kb on - strand, within Dshi_5004 at 2080.549 kb on + strand, within Dshi_5004 at 2080.583 kb on - strand, within Dshi_5004 at 2080.811 kb on - strand, within Dshi_5004
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 40 mM remove 2,076,281 + Dshi_1967 0.30 +1.6 2,076,289 - Dshi_1967 0.31 +1.5 2,076,363 + Dshi_1967 0.37 -0.4 2,076,424 + Dshi_1967 0.41 +1.2 2,076,424 + Dshi_1967 0.41 +1.5 2,076,432 - Dshi_1967 0.42 +1.6 2,076,432 - Dshi_1967 0.42 +0.9 2,076,787 - Dshi_1967 0.69 +2.2 2,076,791 - Dshi_1967 0.70 +0.0 2,077,107 - +1.6 2,077,150 - -1.6 2,077,170 - +3.0 2,077,216 + +0.5 2,077,245 + +0.7 2,077,449 - -0.1 2,077,518 + Dshi_1968 0.10 +1.9 2,077,766 + Dshi_1968 0.20 +1.0 2,077,798 - Dshi_1968 0.21 +3.2 2,077,809 + Dshi_1968 0.21 +1.5 2,077,827 - Dshi_1968 0.22 +1.5 2,077,870 + Dshi_1968 0.24 +0.9 2,078,021 + Dshi_1968 0.29 +1.0 2,078,036 + Dshi_1968 0.30 +1.9 2,078,048 + Dshi_1968 0.30 +1.3 2,078,076 - Dshi_1968 0.31 +0.9 2,078,138 - Dshi_1968 0.34 +3.0 2,078,205 + Dshi_1968 0.36 +0.8 2,078,242 - Dshi_1968 0.38 +2.2 2,078,463 + Dshi_1968 0.46 +2.4 2,078,492 + Dshi_1968 0.47 +2.1 2,078,577 + Dshi_1968 0.50 -0.1 2,078,577 + Dshi_1968 0.50 +0.5 2,078,577 + Dshi_1968 0.50 -0.8 2,078,583 - Dshi_1968 0.50 +1.7 2,078,585 - Dshi_1968 0.51 -2.9 2,078,626 + Dshi_1968 0.52 +0.8 2,078,742 - Dshi_1968 0.56 +2.7 2,078,744 + Dshi_1968 0.57 +1.9 2,078,815 - Dshi_1968 0.59 +2.4 2,078,830 + Dshi_1968 0.60 +1.0 2,078,830 + Dshi_1968 0.60 +1.9 2,078,912 - Dshi_1968 0.63 +1.6 2,079,013 + Dshi_1968 0.67 -1.6 2,079,014 + Dshi_1968 0.67 -1.0 2,079,022 - Dshi_1968 0.67 +1.0 2,079,027 - Dshi_1968 0.67 -0.1 2,079,027 - Dshi_1968 0.67 +2.2 2,079,158 - Dshi_1968 0.72 +3.0 2,079,225 + Dshi_1968 0.75 +1.5 2,079,257 + Dshi_1968 0.76 +2.9 2,079,265 - Dshi_1968 0.76 +0.9 2,079,339 - Dshi_1968 0.79 -0.3 2,079,390 + Dshi_1968 0.81 +2.7 2,079,391 - Dshi_1968 0.81 +2.2 2,079,478 - Dshi_1968 0.84 +1.9 2,079,479 - Dshi_1968 0.84 +2.1 2,079,498 - Dshi_1968 0.85 +1.9 2,079,537 + Dshi_1968 0.86 +0.2 2,079,620 + Dshi_1968 0.89 +1.8 2,079,625 + Dshi_1968 0.90 +1.5 2,079,626 - Dshi_1968 0.90 +1.2 2,079,633 - Dshi_1968 0.90 -1.2 2,079,633 - Dshi_1968 0.90 +2.2 2,079,720 + +1.1 2,079,755 - +1.7 2,079,814 + +1.4 2,079,915 + +2.0 2,079,923 + +1.9 2,079,923 - +0.6 2,079,970 - +0.2 2,080,001 + +1.2 2,080,109 - +1.9 2,080,228 + -0.9 2,080,231 + +0.2 2,080,236 - +1.5 2,080,237 + +1.0 2,080,352 - Dshi_5004 0.13 -2.4 2,080,393 + Dshi_5004 0.15 +1.6 2,080,396 - Dshi_5004 0.16 -0.3 2,080,398 - Dshi_5004 0.16 +1.5 2,080,487 + Dshi_5004 0.21 +0.7 2,080,494 - Dshi_5004 0.21 +0.2 2,080,510 - Dshi_5004 0.22 +0.3 2,080,549 + Dshi_5004 0.25 +0.2 2,080,583 - Dshi_5004 0.27 -0.3 2,080,811 - Dshi_5004 0.40 +0.5
Or see this region's nucleotide sequence