Experiment: m.b. Cholin acetate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1721 and Dshi_1722 are separated by 39 nucleotides Dshi_1722 and Dshi_R0031 are separated by 85 nucleotides Dshi_R0031 and Dshi_1723 are separated by 59 nucleotides Dshi_1723 and Dshi_1724 are separated by 343 nucleotides
Dshi_1721: Dshi_1721 - integration host factor, alpha subunit (RefSeq), at 1,788,324 to 1,788,629
_1721
Dshi_1722: Dshi_1722 - transcriptional regulator, MerR family (RefSeq), at 1,788,669 to 1,789,406
_1722
Dshi_R0031: Dshi_R0031 - tRNA-Pro (RefSeq), at 1,789,492 to 1,789,568
_R0031
Dshi_1723: Dshi_1723 - 2-deoxycytidine 5-triphosphate deaminase (RefSeq), at 1,789,628 to 1,790,722
_1723
Dshi_1724: Dshi_1724 - segregation and condensation protein B (RefSeq), at 1,791,066 to 1,791,755
_1724
Position (kb)
1789
1790
1791 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 1788.778 kb on - strand, within Dshi_1722 at 1789.177 kb on + strand, within Dshi_1722 at 1789.704 kb on - strand at 1789.872 kb on - strand, within Dshi_1723 at 1790.087 kb on + strand, within Dshi_1723 at 1790.417 kb on + strand, within Dshi_1723 at 1790.650 kb on + strand at 1790.691 kb on + strand at 1790.834 kb on - strand at 1790.944 kb on + strand at 1791.022 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 40 mM remove 1,788,778 - Dshi_1722 0.15 +0.3 1,789,177 + Dshi_1722 0.69 -0.5 1,789,704 - -1.8 1,789,872 - Dshi_1723 0.22 -1.7 1,790,087 + Dshi_1723 0.42 -1.3 1,790,417 + Dshi_1723 0.72 -2.0 1,790,650 + +5.7 1,790,691 + +1.5 1,790,834 - -0.5 1,790,944 + -0.8 1,791,022 + +0.4
Or see this region's nucleotide sequence