Experiment: m.b. Cholin acetate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1227 and Dshi_1228 are separated by 83 nucleotides Dshi_1228 and Dshi_1229 are separated by 105 nucleotides Dshi_1229 and Dshi_1230 overlap by 4 nucleotides
Dshi_1227: Dshi_1227 - malate synthase G (RefSeq), at 1,269,474 to 1,271,648
_1227
Dshi_1228: Dshi_1228 - protein of unknown function DUF336 (RefSeq), at 1,271,732 to 1,272,154
_1228
Dshi_1229: Dshi_1229 - peptidase C26 (RefSeq), at 1,272,260 to 1,273,039
_1229
Dshi_1230: Dshi_1230 - kynureninase (RefSeq), at 1,273,036 to 1,274,217
_1230
Position (kb)
1272
1273
1274 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1271.269 kb on + strand, within Dshi_1227 at 1271.275 kb on - strand, within Dshi_1227 at 1271.275 kb on - strand, within Dshi_1227 at 1271.303 kb on - strand, within Dshi_1227 at 1271.313 kb on - strand, within Dshi_1227 at 1271.347 kb on - strand, within Dshi_1227 at 1271.496 kb on - strand at 1271.540 kb on + strand at 1271.641 kb on + strand at 1271.761 kb on + strand at 1271.779 kb on - strand, within Dshi_1228 at 1271.782 kb on + strand, within Dshi_1228 at 1271.869 kb on - strand, within Dshi_1228 at 1271.879 kb on - strand, within Dshi_1228 at 1271.910 kb on - strand, within Dshi_1228 at 1272.121 kb on + strand at 1272.148 kb on + strand at 1272.176 kb on + strand at 1272.184 kb on - strand at 1272.196 kb on + strand at 1272.233 kb on - strand at 1272.395 kb on + strand, within Dshi_1229 at 1272.401 kb on + strand, within Dshi_1229 at 1272.559 kb on + strand, within Dshi_1229 at 1272.567 kb on - strand, within Dshi_1229 at 1272.577 kb on - strand, within Dshi_1229 at 1272.686 kb on + strand, within Dshi_1229 at 1272.795 kb on - strand, within Dshi_1229 at 1272.986 kb on + strand at 1272.986 kb on + strand at 1272.991 kb on + strand at 1272.991 kb on + strand at 1272.994 kb on - strand at 1273.142 kb on - strand at 1273.344 kb on + strand, within Dshi_1230 at 1273.357 kb on + strand, within Dshi_1230 at 1273.567 kb on - strand, within Dshi_1230 at 1273.610 kb on - strand, within Dshi_1230 at 1273.646 kb on - strand, within Dshi_1230 at 1273.710 kb on - strand, within Dshi_1230 at 1273.834 kb on + strand, within Dshi_1230 at 1273.864 kb on + strand, within Dshi_1230 at 1273.991 kb on + strand, within Dshi_1230
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 40 mM remove 1,271,269 + Dshi_1227 0.83 -1.2 1,271,275 - Dshi_1227 0.83 +2.1 1,271,275 - Dshi_1227 0.83 +0.1 1,271,303 - Dshi_1227 0.84 -2.2 1,271,313 - Dshi_1227 0.85 -1.2 1,271,347 - Dshi_1227 0.86 +0.4 1,271,496 - +0.9 1,271,540 + +0.6 1,271,641 + +0.1 1,271,761 + +0.6 1,271,779 - Dshi_1228 0.11 -0.8 1,271,782 + Dshi_1228 0.12 -0.3 1,271,869 - Dshi_1228 0.32 -2.2 1,271,879 - Dshi_1228 0.35 +0.5 1,271,910 - Dshi_1228 0.42 -0.9 1,272,121 + +0.7 1,272,148 + +1.2 1,272,176 + +0.5 1,272,184 - +0.7 1,272,196 + +1.3 1,272,233 - +0.7 1,272,395 + Dshi_1229 0.17 +0.5 1,272,401 + Dshi_1229 0.18 +0.1 1,272,559 + Dshi_1229 0.38 -0.6 1,272,567 - Dshi_1229 0.39 -0.5 1,272,577 - Dshi_1229 0.41 -1.0 1,272,686 + Dshi_1229 0.55 -1.1 1,272,795 - Dshi_1229 0.69 -1.6 1,272,986 + +1.3 1,272,986 + +2.0 1,272,991 + +0.8 1,272,991 + -1.3 1,272,994 - -1.8 1,273,142 - -0.6 1,273,344 + Dshi_1230 0.26 -1.5 1,273,357 + Dshi_1230 0.27 -1.0 1,273,567 - Dshi_1230 0.45 -1.3 1,273,610 - Dshi_1230 0.49 +0.3 1,273,646 - Dshi_1230 0.52 -1.3 1,273,710 - Dshi_1230 0.57 +0.6 1,273,834 + Dshi_1230 0.68 -0.4 1,273,864 + Dshi_1230 0.70 -1.0 1,273,991 + Dshi_1230 0.81 -2.5
Or see this region's nucleotide sequence