Strain Fitness in Dinoroseobacter shibae DFL-12 around Dshi_0700

Experiment: m.b. Cholin acetate 40 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntDshi_0699 and Dshi_0700 are separated by 59 nucleotidesDshi_0700 and Dshi_0701 are separated by 52 nucleotidesDshi_0701 and Dshi_0702 overlap by 4 nucleotides Dshi_0699: Dshi_0699 - TRAP C4-dicarboxylate transport system permease DctM subunit (RefSeq), at 705,226 to 706,539 _0699 Dshi_0700: Dshi_0700 - AMP-dependent synthetase and ligase (RefSeq), at 706,599 to 708,116 _0700 Dshi_0701: Dshi_0701 - Methyltransferase type 12 (RefSeq), at 708,169 to 708,822 _0701 Dshi_0702: Dshi_0702 - WbqC-like family protein (RefSeq), at 708,819 to 709,466 _0702 Position (kb) 706 707 708 709Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 705.610 kb on - strand, within Dshi_0699at 705.626 kb on - strand, within Dshi_0699at 706.010 kb on - strand, within Dshi_0699at 706.023 kb on + strand, within Dshi_0699at 706.183 kb on - strand, within Dshi_0699at 706.212 kb on + strand, within Dshi_0699at 706.315 kb on - strand, within Dshi_0699at 706.320 kb on - strand, within Dshi_0699at 706.325 kb on - strand, within Dshi_0699at 706.406 kb on - strand, within Dshi_0699at 706.453 kb on - strandat 706.453 kb on - strandat 706.535 kb on + strandat 706.557 kb on + strandat 706.557 kb on + strandat 706.557 kb on + strandat 706.565 kb on - strandat 706.565 kb on - strandat 706.565 kb on - strandat 706.565 kb on - strandat 706.624 kb on + strandat 706.627 kb on - strandat 706.715 kb on + strandat 706.741 kb on + strandat 706.745 kb on + strandat 706.745 kb on + strandat 706.753 kb on - strand, within Dshi_0700at 706.802 kb on + strand, within Dshi_0700at 706.820 kb on + strand, within Dshi_0700at 706.846 kb on + strand, within Dshi_0700at 706.880 kb on - strand, within Dshi_0700at 706.899 kb on + strand, within Dshi_0700at 706.931 kb on + strand, within Dshi_0700at 706.941 kb on - strand, within Dshi_0700at 706.947 kb on + strand, within Dshi_0700at 707.065 kb on - strand, within Dshi_0700at 707.084 kb on + strand, within Dshi_0700at 707.084 kb on + strand, within Dshi_0700at 707.084 kb on + strand, within Dshi_0700at 707.092 kb on - strand, within Dshi_0700at 707.163 kb on - strand, within Dshi_0700at 707.185 kb on - strand, within Dshi_0700at 707.211 kb on - strand, within Dshi_0700at 707.213 kb on + strand, within Dshi_0700at 707.224 kb on + strand, within Dshi_0700at 707.268 kb on + strand, within Dshi_0700at 707.400 kb on + strand, within Dshi_0700at 707.615 kb on + strand, within Dshi_0700at 707.643 kb on - strand, within Dshi_0700at 707.684 kb on + strand, within Dshi_0700at 707.736 kb on + strand, within Dshi_0700at 707.741 kb on + strand, within Dshi_0700at 707.741 kb on + strand, within Dshi_0700at 707.842 kb on - strand, within Dshi_0700at 707.870 kb on - strand, within Dshi_0700at 707.872 kb on + strand, within Dshi_0700at 707.931 kb on + strand, within Dshi_0700at 707.939 kb on - strand, within Dshi_0700at 707.997 kb on - strandat 707.997 kb on - strandat 708.014 kb on + strandat 708.031 kb on - strandat 708.031 kb on - strandat 708.075 kb on + strandat 708.082 kb on + strandat 708.105 kb on + strandat 708.260 kb on - strand, within Dshi_0701at 708.361 kb on + strand, within Dshi_0701at 708.377 kb on + strand, within Dshi_0701at 708.380 kb on - strand, within Dshi_0701at 708.384 kb on - strand, within Dshi_0701at 708.539 kb on + strand, within Dshi_0701at 708.582 kb on + strand, within Dshi_0701at 708.673 kb on + strand, within Dshi_0701at 708.809 kb on - strandat 708.945 kb on + strand, within Dshi_0702at 708.945 kb on + strand, within Dshi_0702at 709.034 kb on - strand, within Dshi_0702

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Per-strain Table

Position Strand Gene LocusTag Fraction m.b. Cholin acetate 40 mM
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705,610 - Dshi_0699 0.29 +2.2
705,626 - Dshi_0699 0.30 -0.0
706,010 - Dshi_0699 0.60 -0.8
706,023 + Dshi_0699 0.61 -2.2
706,183 - Dshi_0699 0.73 -0.2
706,212 + Dshi_0699 0.75 +0.3
706,315 - Dshi_0699 0.83 -0.8
706,320 - Dshi_0699 0.83 +2.1
706,325 - Dshi_0699 0.84 -2.5
706,406 - Dshi_0699 0.90 +1.8
706,453 - +3.2
706,453 - -3.4
706,535 + +1.8
706,557 + +1.5
706,557 + +1.3
706,557 + -1.5
706,565 - -2.2
706,565 - +0.9
706,565 - -0.1
706,565 - -1.8
706,624 + -1.2
706,627 - -0.9
706,715 + +1.2
706,741 + -1.3
706,745 + +0.8
706,745 + +0.8
706,753 - Dshi_0700 0.10 +0.5
706,802 + Dshi_0700 0.13 -0.7
706,820 + Dshi_0700 0.15 +0.6
706,846 + Dshi_0700 0.16 -2.7
706,880 - Dshi_0700 0.19 +0.8
706,899 + Dshi_0700 0.20 -1.3
706,931 + Dshi_0700 0.22 -0.0
706,941 - Dshi_0700 0.23 +0.6
706,947 + Dshi_0700 0.23 +0.4
707,065 - Dshi_0700 0.31 +0.4
707,084 + Dshi_0700 0.32 +0.2
707,084 + Dshi_0700 0.32 -2.5
707,084 + Dshi_0700 0.32 +0.3
707,092 - Dshi_0700 0.32 +0.4
707,163 - Dshi_0700 0.37 -1.2
707,185 - Dshi_0700 0.39 -0.8
707,211 - Dshi_0700 0.40 +2.6
707,213 + Dshi_0700 0.40 -1.9
707,224 + Dshi_0700 0.41 -0.4
707,268 + Dshi_0700 0.44 +0.2
707,400 + Dshi_0700 0.53 -0.9
707,615 + Dshi_0700 0.67 -1.5
707,643 - Dshi_0700 0.69 -1.9
707,684 + Dshi_0700 0.71 +1.2
707,736 + Dshi_0700 0.75 +2.0
707,741 + Dshi_0700 0.75 -2.2
707,741 + Dshi_0700 0.75 -1.0
707,842 - Dshi_0700 0.82 -1.5
707,870 - Dshi_0700 0.84 +1.8
707,872 + Dshi_0700 0.84 -0.6
707,931 + Dshi_0700 0.88 -2.6
707,939 - Dshi_0700 0.88 -1.2
707,997 - +1.1
707,997 - -1.0
708,014 + -1.4
708,031 - -0.9
708,031 - -0.1
708,075 + +0.7
708,082 + +4.2
708,105 + +0.0
708,260 - Dshi_0701 0.14 -1.2
708,361 + Dshi_0701 0.29 -0.8
708,377 + Dshi_0701 0.32 -0.5
708,380 - Dshi_0701 0.32 +1.0
708,384 - Dshi_0701 0.33 +0.6
708,539 + Dshi_0701 0.57 -2.4
708,582 + Dshi_0701 0.63 -0.3
708,673 + Dshi_0701 0.77 -0.3
708,809 - +0.1
708,945 + Dshi_0702 0.19 -0.4
708,945 + Dshi_0702 0.19 -0.2
709,034 - Dshi_0702 0.33 -0.3

Or see this region's nucleotide sequence