Experiment: m.b. Cholin acetate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0617 and Dshi_0618 are separated by 44 nucleotides Dshi_0618 and Dshi_0619 are separated by 228 nucleotides Dshi_0619 and Dshi_0620 overlap by 4 nucleotides
Dshi_0617: Dshi_0617 - ATPase AAA-2 domain protein (RefSeq), at 609,070 to 611,685
_0617
Dshi_0618: Dshi_0618 - hypothetical protein (RefSeq), at 611,730 to 612,320
_0618
Dshi_0619: Dshi_0619 - hypothetical protein (RefSeq), at 612,549 to 612,863
_0619
Dshi_0620: Dshi_0620 - short-chain dehydrogenase/reductase SDR (RefSeq), at 612,860 to 613,612
_0620
Position (kb)
611
612
613 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 610.758 kb on - strand, within Dshi_0617 at 610.841 kb on - strand, within Dshi_0617 at 610.905 kb on + strand, within Dshi_0617 at 610.905 kb on + strand, within Dshi_0617 at 611.011 kb on + strand, within Dshi_0617 at 611.019 kb on - strand, within Dshi_0617 at 611.020 kb on - strand, within Dshi_0617 at 611.049 kb on + strand, within Dshi_0617 at 611.155 kb on + strand, within Dshi_0617 at 611.180 kb on + strand, within Dshi_0617 at 611.221 kb on - strand, within Dshi_0617 at 611.262 kb on + strand, within Dshi_0617 at 611.430 kb on - strand at 611.432 kb on + strand at 611.436 kb on + strand at 611.490 kb on - strand at 611.542 kb on + strand at 611.707 kb on + strand at 611.775 kb on + strand at 611.863 kb on + strand, within Dshi_0618 at 611.974 kb on - strand, within Dshi_0618 at 612.296 kb on - strand at 612.376 kb on + strand at 612.383 kb on + strand at 612.391 kb on - strand at 612.391 kb on - strand at 612.442 kb on + strand at 612.526 kb on + strand at 612.527 kb on + strand at 612.600 kb on - strand, within Dshi_0619 at 612.651 kb on - strand, within Dshi_0619 at 612.673 kb on + strand, within Dshi_0619 at 612.707 kb on - strand, within Dshi_0619 at 612.746 kb on + strand, within Dshi_0619 at 612.811 kb on + strand, within Dshi_0619 at 612.819 kb on - strand, within Dshi_0619 at 612.848 kb on - strand at 612.915 kb on + strand at 613.009 kb on - strand, within Dshi_0620 at 613.053 kb on + strand, within Dshi_0620 at 613.078 kb on + strand, within Dshi_0620 at 613.088 kb on + strand, within Dshi_0620 at 613.120 kb on + strand, within Dshi_0620 at 613.128 kb on + strand, within Dshi_0620 at 613.130 kb on - strand, within Dshi_0620 at 613.169 kb on + strand, within Dshi_0620 at 613.186 kb on + strand, within Dshi_0620 at 613.188 kb on + strand, within Dshi_0620 at 613.188 kb on + strand, within Dshi_0620 at 613.226 kb on - strand, within Dshi_0620 at 613.249 kb on + strand, within Dshi_0620
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 40 mM remove 610,758 - Dshi_0617 0.65 +0.5 610,841 - Dshi_0617 0.68 -2.4 610,905 + Dshi_0617 0.70 +0.3 610,905 + Dshi_0617 0.70 +1.0 611,011 + Dshi_0617 0.74 +0.1 611,019 - Dshi_0617 0.75 +0.2 611,020 - Dshi_0617 0.75 -3.3 611,049 + Dshi_0617 0.76 -0.8 611,155 + Dshi_0617 0.80 +0.2 611,180 + Dshi_0617 0.81 -1.4 611,221 - Dshi_0617 0.82 +0.6 611,262 + Dshi_0617 0.84 +2.2 611,430 - +0.2 611,432 + -1.8 611,436 + -0.2 611,490 - +0.8 611,542 + +1.4 611,707 + -2.8 611,775 + +1.0 611,863 + Dshi_0618 0.23 -0.7 611,974 - Dshi_0618 0.41 -2.1 612,296 - -2.3 612,376 + +1.4 612,383 + -0.4 612,391 - -1.0 612,391 - -0.4 612,442 + -0.2 612,526 + -0.5 612,527 + +0.6 612,600 - Dshi_0619 0.16 -0.2 612,651 - Dshi_0619 0.32 +2.2 612,673 + Dshi_0619 0.39 -0.2 612,707 - Dshi_0619 0.50 -0.4 612,746 + Dshi_0619 0.63 +2.2 612,811 + Dshi_0619 0.83 -0.0 612,819 - Dshi_0619 0.86 -0.3 612,848 - +0.1 612,915 + -0.7 613,009 - Dshi_0620 0.20 +0.0 613,053 + Dshi_0620 0.26 -0.7 613,078 + Dshi_0620 0.29 -1.8 613,088 + Dshi_0620 0.30 +0.2 613,120 + Dshi_0620 0.35 +0.7 613,128 + Dshi_0620 0.36 -1.3 613,130 - Dshi_0620 0.36 +0.9 613,169 + Dshi_0620 0.41 +0.8 613,186 + Dshi_0620 0.43 +0.7 613,188 + Dshi_0620 0.44 -1.1 613,188 + Dshi_0620 0.44 +0.2 613,226 - Dshi_0620 0.49 +0.5 613,249 + Dshi_0620 0.52 -0.6
Or see this region's nucleotide sequence