Experiment: m.b. Cholin acetate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0558 and Dshi_0559 are separated by 446 nucleotides Dshi_0559 and Dshi_0560 overlap by 4 nucleotides Dshi_0560 and Dshi_0561 are separated by 15 nucleotides
Dshi_0558: Dshi_0558 - D-amino-acid dehydrogenase (RefSeq), at 545,054 to 546,313
_0558
Dshi_0559: Dshi_0559 - protein of unknown function DUF1636 (RefSeq), at 546,760 to 547,167
_0559
Dshi_0560: Dshi_0560 - ABC transporter related (RefSeq), at 547,164 to 547,937
_0560
Dshi_0561: Dshi_0561 - periplasmic binding protein (RefSeq), at 547,953 to 548,888
_0561
Position (kb)
546
547
548 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 545.760 kb on - strand, within Dshi_0558 at 545.760 kb on - strand, within Dshi_0558 at 545.798 kb on + strand, within Dshi_0558 at 545.799 kb on + strand, within Dshi_0558 at 545.804 kb on + strand, within Dshi_0558 at 545.806 kb on - strand, within Dshi_0558 at 545.810 kb on + strand, within Dshi_0558 at 545.816 kb on - strand, within Dshi_0558 at 545.878 kb on + strand, within Dshi_0558 at 545.954 kb on + strand, within Dshi_0558 at 546.100 kb on + strand, within Dshi_0558 at 546.176 kb on - strand, within Dshi_0558 at 546.213 kb on - strand at 546.258 kb on - strand at 546.294 kb on - strand at 546.340 kb on - strand at 546.340 kb on - strand at 546.361 kb on + strand at 546.361 kb on + strand at 546.369 kb on - strand at 546.381 kb on + strand at 546.381 kb on + strand at 546.389 kb on - strand at 546.422 kb on + strand at 546.458 kb on + strand at 546.527 kb on + strand at 546.527 kb on + strand at 546.556 kb on + strand at 546.556 kb on + strand at 546.564 kb on - strand at 546.564 kb on - strand at 546.589 kb on - strand at 546.589 kb on - strand at 546.605 kb on + strand at 546.613 kb on - strand at 546.730 kb on - strand at 546.753 kb on + strand at 546.839 kb on + strand, within Dshi_0559 at 546.842 kb on - strand, within Dshi_0559 at 546.847 kb on - strand, within Dshi_0559 at 546.847 kb on - strand, within Dshi_0559 at 546.878 kb on - strand, within Dshi_0559 at 546.924 kb on - strand, within Dshi_0559 at 546.978 kb on + strand, within Dshi_0559 at 546.998 kb on - strand, within Dshi_0559 at 547.017 kb on - strand, within Dshi_0559 at 547.045 kb on - strand, within Dshi_0559 at 547.111 kb on + strand, within Dshi_0559 at 547.114 kb on - strand, within Dshi_0559 at 547.119 kb on - strand, within Dshi_0559 at 547.240 kb on + strand at 547.253 kb on + strand, within Dshi_0560 at 547.297 kb on + strand, within Dshi_0560 at 547.305 kb on - strand, within Dshi_0560 at 547.338 kb on + strand, within Dshi_0560 at 547.388 kb on - strand, within Dshi_0560 at 547.456 kb on - strand at 547.499 kb on + strand, within Dshi_0560 at 547.499 kb on + strand, within Dshi_0560 at 547.502 kb on - strand, within Dshi_0560 at 547.512 kb on - strand, within Dshi_0560 at 547.514 kb on - strand, within Dshi_0560 at 547.514 kb on - strand, within Dshi_0560 at 547.710 kb on + strand, within Dshi_0560 at 547.723 kb on - strand, within Dshi_0560 at 547.723 kb on - strand, within Dshi_0560 at 547.782 kb on + strand, within Dshi_0560 at 547.795 kb on - strand, within Dshi_0560 at 547.815 kb on - strand, within Dshi_0560 at 547.820 kb on - strand, within Dshi_0560 at 547.851 kb on + strand, within Dshi_0560 at 547.887 kb on + strand at 547.895 kb on - strand at 547.902 kb on - strand at 547.902 kb on - strand at 547.906 kb on + strand at 547.919 kb on + strand at 547.925 kb on - strand at 547.925 kb on - strand at 547.969 kb on + strand at 547.975 kb on + strand at 547.985 kb on - strand at 548.019 kb on + strand at 548.080 kb on + strand, within Dshi_0561
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 40 mM remove 545,760 - Dshi_0558 0.56 -1.6 545,760 - Dshi_0558 0.56 -0.9 545,798 + Dshi_0558 0.59 +1.4 545,799 + Dshi_0558 0.59 +0.3 545,804 + Dshi_0558 0.60 -0.2 545,806 - Dshi_0558 0.60 -0.1 545,810 + Dshi_0558 0.60 -1.8 545,816 - Dshi_0558 0.60 -0.2 545,878 + Dshi_0558 0.65 +0.9 545,954 + Dshi_0558 0.71 -0.6 546,100 + Dshi_0558 0.83 +0.2 546,176 - Dshi_0558 0.89 +0.9 546,213 - +0.4 546,258 - -0.1 546,294 - -0.8 546,340 - -0.1 546,340 - -2.3 546,361 + -0.2 546,361 + +1.8 546,369 - +0.3 546,381 + +1.2 546,381 + +0.4 546,389 - -0.5 546,422 + -0.0 546,458 + -0.9 546,527 + +0.8 546,527 + -2.1 546,556 + -0.5 546,556 + +0.1 546,564 - -1.1 546,564 - -0.9 546,589 - -0.9 546,589 - -0.0 546,605 + -0.2 546,613 - -0.6 546,730 - -0.1 546,753 + -0.3 546,839 + Dshi_0559 0.19 -0.4 546,842 - Dshi_0559 0.20 -2.0 546,847 - Dshi_0559 0.21 -0.3 546,847 - Dshi_0559 0.21 -0.4 546,878 - Dshi_0559 0.29 +0.4 546,924 - Dshi_0559 0.40 -1.1 546,978 + Dshi_0559 0.53 -1.9 546,998 - Dshi_0559 0.58 -1.5 547,017 - Dshi_0559 0.63 -0.2 547,045 - Dshi_0559 0.70 -1.2 547,111 + Dshi_0559 0.86 +0.5 547,114 - Dshi_0559 0.87 +0.2 547,119 - Dshi_0559 0.88 -0.9 547,240 + -0.5 547,253 + Dshi_0560 0.11 -1.5 547,297 + Dshi_0560 0.17 -0.6 547,305 - Dshi_0560 0.18 -1.9 547,338 + Dshi_0560 0.22 +0.1 547,388 - Dshi_0560 0.29 +0.0 547,456 - -0.9 547,499 + Dshi_0560 0.43 -1.2 547,499 + Dshi_0560 0.43 -0.3 547,502 - Dshi_0560 0.44 -1.3 547,512 - Dshi_0560 0.45 -0.1 547,514 - Dshi_0560 0.45 -0.7 547,514 - Dshi_0560 0.45 +0.7 547,710 + Dshi_0560 0.71 +0.4 547,723 - Dshi_0560 0.72 -0.6 547,723 - Dshi_0560 0.72 -0.3 547,782 + Dshi_0560 0.80 -0.5 547,795 - Dshi_0560 0.82 +0.0 547,815 - Dshi_0560 0.84 -0.2 547,820 - Dshi_0560 0.85 -2.1 547,851 + Dshi_0560 0.89 +0.0 547,887 + -1.7 547,895 - -1.0 547,902 - -0.6 547,902 - -0.0 547,906 + +0.1 547,919 + -0.3 547,925 - -1.3 547,925 - +0.4 547,969 + +1.0 547,975 + -2.2 547,985 - -1.6 548,019 + -0.9 548,080 + Dshi_0561 0.14 +1.6
Or see this region's nucleotide sequence