Experiment: m.b. Cholin acetate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0181 and Dshi_0182 are separated by 104 nucleotides Dshi_0182 and Dshi_0183 overlap by 8 nucleotides Dshi_0183 and Dshi_0184 overlap by 4 nucleotides Dshi_0184 and Dshi_0185 are separated by 39 nucleotides
Dshi_0181: Dshi_0181 - methionyl-tRNA formyltransferase (RefSeq), at 184,186 to 185,085
_0181
Dshi_0182: Dshi_0182 - ribonuclease H (RefSeq), at 185,190 to 185,663
_0182
Dshi_0183: Dshi_0183 - Methyltransferase type 11 (RefSeq), at 185,656 to 186,255
_0183
Dshi_0184: Dshi_0184 - hypothetical protein (RefSeq), at 186,252 to 186,704
_0184
Dshi_0185: Dshi_0185 - phosphatidate cytidylyltransferase (RefSeq), at 186,744 to 187,694
_0185
Position (kb) 185
186
187 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 185.057 kb on + strand at 185.109 kb on + strand at 185.189 kb on - strand at 185.597 kb on - strand, within Dshi_0182 at 185.678 kb on - strand at 185.801 kb on + strand, within Dshi_0183 at 185.801 kb on + strand, within Dshi_0183 at 185.844 kb on + strand, within Dshi_0183 at 186.169 kb on - strand, within Dshi_0183 at 186.425 kb on + strand, within Dshi_0184 at 186.627 kb on - strand, within Dshi_0184 at 186.741 kb on + strand at 186.749 kb on - strand at 186.749 kb on - strand at 186.855 kb on + strand, within Dshi_0185 at 186.952 kb on + strand, within Dshi_0185 at 186.952 kb on + strand, within Dshi_0185 at 186.977 kb on + strand, within Dshi_0185 at 187.004 kb on - strand, within Dshi_0185 at 187.065 kb on - strand, within Dshi_0185 at 187.140 kb on - strand, within Dshi_0185
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 40 mM remove 185,057 + +2.2 185,109 + +0.6 185,189 - -0.1 185,597 - Dshi_0182 0.86 -0.5 185,678 - -0.3 185,801 + Dshi_0183 0.24 +1.6 185,801 + Dshi_0183 0.24 +0.6 185,844 + Dshi_0183 0.31 +0.3 186,169 - Dshi_0183 0.85 +2.8 186,425 + Dshi_0184 0.38 -2.2 186,627 - Dshi_0184 0.83 -0.8 186,741 + +0.4 186,749 - -1.8 186,749 - -1.1 186,855 + Dshi_0185 0.12 -1.6 186,952 + Dshi_0185 0.22 +0.2 186,952 + Dshi_0185 0.22 +0.4 186,977 + Dshi_0185 0.25 +0.4 187,004 - Dshi_0185 0.27 -0.6 187,065 - Dshi_0185 0.34 +1.0 187,140 - Dshi_0185 0.42 -1.8
Or see this region's nucleotide sequence