Experiment: m.b. Cholin acetate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0002 and Dshi_0004 are separated by 936 nucleotides Dshi_0004 and Dshi_0005 are separated by 105 nucleotides Dshi_0005 and Dshi_0006 are separated by 103 nucleotides
Dshi_0002: Dshi_0002 - Glutamate synthase (ferredoxin) (RefSeq), at 1,782 to 6,320
_0002
Dshi_0004: Dshi_0004 - hypothetical protein (RefSeq), at 7,257 to 8,114
_0004
Dshi_0005: Dshi_0005 - hypothetical protein (RefSeq), at 8,220 to 8,924
_0005
Dshi_0006: Dshi_0006 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (RefSeq), at 9,028 to 9,855
_0006
Position (kb)
7
8
9 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 6.258 kb on - strand at 6.267 kb on + strand at 6.277 kb on - strand at 6.284 kb on + strand at 6.284 kb on + strand at 6.291 kb on + strand at 6.291 kb on + strand at 6.299 kb on - strand at 6.299 kb on - strand at 6.309 kb on - strand at 6.315 kb on + strand at 6.383 kb on + strand at 6.910 kb on + strand at 6.954 kb on - strand at 6.971 kb on - strand at 6.972 kb on + strand at 6.972 kb on - strand at 6.987 kb on - strand at 6.996 kb on + strand at 6.996 kb on + strand at 6.996 kb on + strand at 7.012 kb on + strand at 7.030 kb on + strand at 7.073 kb on - strand at 7.073 kb on - strand at 7.073 kb on - strand at 7.082 kb on + strand at 7.083 kb on - strand at 7.083 kb on - strand at 7.133 kb on + strand at 7.141 kb on - strand at 7.174 kb on + strand at 7.182 kb on - strand at 7.182 kb on - strand at 7.223 kb on - strand at 7.234 kb on + strand at 7.241 kb on - strand at 7.245 kb on + strand at 7.245 kb on + strand at 7.253 kb on - strand at 7.253 kb on - strand at 7.257 kb on + strand at 7.270 kb on - strand at 7.270 kb on - strand at 7.275 kb on - strand at 7.297 kb on + strand at 7.297 kb on + strand at 7.307 kb on + strand at 7.312 kb on + strand at 7.320 kb on - strand at 7.320 kb on - strand at 7.325 kb on - strand at 7.342 kb on - strand at 7.349 kb on - strand, within Dshi_0004 at 7.351 kb on + strand, within Dshi_0004 at 7.362 kb on + strand, within Dshi_0004 at 7.373 kb on + strand, within Dshi_0004 at 7.418 kb on - strand, within Dshi_0004 at 7.457 kb on + strand, within Dshi_0004 at 7.474 kb on + strand, within Dshi_0004 at 7.474 kb on + strand, within Dshi_0004 at 7.474 kb on + strand, within Dshi_0004 at 7.482 kb on - strand, within Dshi_0004 at 7.482 kb on - strand, within Dshi_0004 at 7.485 kb on + strand, within Dshi_0004 at 7.486 kb on - strand, within Dshi_0004 at 7.486 kb on - strand, within Dshi_0004 at 7.537 kb on + strand, within Dshi_0004 at 7.615 kb on + strand, within Dshi_0004 at 7.615 kb on + strand, within Dshi_0004 at 7.720 kb on - strand, within Dshi_0004 at 7.720 kb on - strand, within Dshi_0004 at 7.749 kb on + strand, within Dshi_0004 at 7.810 kb on - strand, within Dshi_0004 at 7.810 kb on - strand, within Dshi_0004 at 7.821 kb on - strand, within Dshi_0004 at 7.843 kb on + strand, within Dshi_0004 at 7.870 kb on - strand, within Dshi_0004 at 7.933 kb on + strand, within Dshi_0004 at 7.998 kb on + strand, within Dshi_0004 at 8.028 kb on + strand, within Dshi_0004 at 8.028 kb on + strand, within Dshi_0004 at 8.028 kb on + strand, within Dshi_0004 at 8.028 kb on + strand, within Dshi_0004 at 8.031 kb on - strand at 8.036 kb on - strand at 8.036 kb on - strand at 8.036 kb on - strand at 8.036 kb on - strand at 8.048 kb on - strand at 8.085 kb on + strand at 8.117 kb on - strand at 8.141 kb on - strand at 8.205 kb on - strand at 8.259 kb on + strand at 8.351 kb on + strand, within Dshi_0005 at 8.449 kb on - strand, within Dshi_0005 at 8.569 kb on + strand, within Dshi_0005 at 8.587 kb on - strand, within Dshi_0005 at 8.647 kb on + strand, within Dshi_0005 at 8.683 kb on + strand, within Dshi_0005 at 8.828 kb on - strand, within Dshi_0005 at 8.830 kb on + strand, within Dshi_0005
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 40 mM remove 6,258 - +0.8 6,267 + -0.2 6,277 - +1.1 6,284 + +0.6 6,284 + -0.5 6,291 + +0.8 6,291 + -0.4 6,299 - +3.8 6,299 - +0.3 6,309 - -0.5 6,315 + -0.8 6,383 + +1.0 6,910 + +1.0 6,954 - +0.6 6,971 - -2.0 6,972 + +0.3 6,972 - +1.7 6,987 - +0.1 6,996 + -2.4 6,996 + -0.5 6,996 + -1.8 7,012 + -0.6 7,030 + -0.6 7,073 - +2.2 7,073 - -1.2 7,073 - -2.0 7,082 + -1.5 7,083 - +0.8 7,083 - +0.9 7,133 + -1.2 7,141 - +0.1 7,174 + +1.8 7,182 - +0.1 7,182 - +2.4 7,223 - -1.4 7,234 + -1.1 7,241 - -0.4 7,245 + +1.1 7,245 + -0.2 7,253 - +0.2 7,253 - +0.6 7,257 + +2.6 7,270 - +1.5 7,270 - -2.6 7,275 - +0.2 7,297 + -0.0 7,297 + +0.5 7,307 + -0.3 7,312 + -0.2 7,320 - -0.2 7,320 - +0.4 7,325 - -0.4 7,342 - -2.8 7,349 - Dshi_0004 0.11 +0.4 7,351 + Dshi_0004 0.11 +0.2 7,362 + Dshi_0004 0.12 -0.2 7,373 + Dshi_0004 0.14 -0.7 7,418 - Dshi_0004 0.19 +0.1 7,457 + Dshi_0004 0.23 +0.1 7,474 + Dshi_0004 0.25 -2.1 7,474 + Dshi_0004 0.25 +1.2 7,474 + Dshi_0004 0.25 +2.6 7,482 - Dshi_0004 0.26 -1.4 7,482 - Dshi_0004 0.26 -0.3 7,485 + Dshi_0004 0.27 +0.1 7,486 - Dshi_0004 0.27 -1.3 7,486 - Dshi_0004 0.27 +0.5 7,537 + Dshi_0004 0.33 -0.1 7,615 + Dshi_0004 0.42 +0.1 7,615 + Dshi_0004 0.42 +0.9 7,720 - Dshi_0004 0.54 +0.3 7,720 - Dshi_0004 0.54 +0.9 7,749 + Dshi_0004 0.57 +1.2 7,810 - Dshi_0004 0.64 -0.9 7,810 - Dshi_0004 0.64 -0.1 7,821 - Dshi_0004 0.66 +1.1 7,843 + Dshi_0004 0.68 -1.3 7,870 - Dshi_0004 0.71 -1.0 7,933 + Dshi_0004 0.79 +3.2 7,998 + Dshi_0004 0.86 +0.1 8,028 + Dshi_0004 0.90 +0.9 8,028 + Dshi_0004 0.90 -1.8 8,028 + Dshi_0004 0.90 -0.4 8,028 + Dshi_0004 0.90 +0.6 8,031 - +2.0 8,036 - -2.9 8,036 - -1.9 8,036 - -0.2 8,036 - +0.3 8,048 - -0.4 8,085 + +1.2 8,117 - -0.9 8,141 - +1.4 8,205 - +0.4 8,259 + +2.5 8,351 + Dshi_0005 0.19 +0.2 8,449 - Dshi_0005 0.32 +0.9 8,569 + Dshi_0005 0.50 +0.6 8,587 - Dshi_0005 0.52 +0.4 8,647 + Dshi_0005 0.61 -0.4 8,683 + Dshi_0005 0.66 +1.2 8,828 - Dshi_0005 0.86 +0.2 8,830 + Dshi_0005 0.87 +1.1
Or see this region's nucleotide sequence