Experiment: Urea (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2781 and Pf6N2E2_2782 are separated by 22 nucleotides Pf6N2E2_2782 and Pf6N2E2_2783 are separated by 215 nucleotides Pf6N2E2_2783 and Pf6N2E2_2784 overlap by 8 nucleotides
Pf6N2E2_2781 - Urea carboxylase (EC 6.3.4.6), at 3,267,741 to 3,271,421
_2781
Pf6N2E2_2782 - Allophanate hydrolase (EC 3.5.1.54), at 3,271,444 to 3,273,222
_2782
Pf6N2E2_2783 - Sensory box/GGDEF family protein, at 3,273,438 to 3,274,184
_2783
Pf6N2E2_2784 - DNA or RNA helicases of superfamily II, at 3,274,177 to 3,274,395
_2784
Position (kb)
3271
3272
3273
3274 Strain fitness (log2 ratio)
-1
0
1
2 at 3270.538 kb on - strand, within Pf6N2E2_2781 at 3270.654 kb on + strand, within Pf6N2E2_2781 at 3270.655 kb on - strand, within Pf6N2E2_2781 at 3270.682 kb on - strand, within Pf6N2E2_2781 at 3270.741 kb on + strand, within Pf6N2E2_2781 at 3270.741 kb on + strand, within Pf6N2E2_2781 at 3270.741 kb on + strand, within Pf6N2E2_2781 at 3270.741 kb on + strand, within Pf6N2E2_2781 at 3270.741 kb on + strand, within Pf6N2E2_2781 at 3270.742 kb on - strand, within Pf6N2E2_2781 at 3270.742 kb on - strand, within Pf6N2E2_2781 at 3270.742 kb on - strand, within Pf6N2E2_2781 at 3270.742 kb on - strand, within Pf6N2E2_2781 at 3270.742 kb on - strand, within Pf6N2E2_2781 at 3270.742 kb on - strand, within Pf6N2E2_2781 at 3270.742 kb on - strand, within Pf6N2E2_2781 at 3270.742 kb on - strand, within Pf6N2E2_2781 at 3270.795 kb on + strand, within Pf6N2E2_2781 at 3270.795 kb on + strand, within Pf6N2E2_2781 at 3270.795 kb on + strand, within Pf6N2E2_2781 at 3270.795 kb on + strand, within Pf6N2E2_2781 at 3270.796 kb on - strand, within Pf6N2E2_2781 at 3270.796 kb on - strand, within Pf6N2E2_2781 at 3270.796 kb on - strand, within Pf6N2E2_2781 at 3270.796 kb on - strand, within Pf6N2E2_2781 at 3270.796 kb on - strand, within Pf6N2E2_2781 at 3270.796 kb on - strand, within Pf6N2E2_2781 at 3270.796 kb on - strand, within Pf6N2E2_2781 at 3270.796 kb on - strand, within Pf6N2E2_2781 at 3270.796 kb on - strand, within Pf6N2E2_2781 at 3270.796 kb on - strand, within Pf6N2E2_2781 at 3270.796 kb on - strand, within Pf6N2E2_2781 at 3270.796 kb on - strand, within Pf6N2E2_2781 at 3270.797 kb on + strand, within Pf6N2E2_2781 at 3270.797 kb on + strand, within Pf6N2E2_2781 at 3270.797 kb on + strand, within Pf6N2E2_2781 at 3270.797 kb on + strand, within Pf6N2E2_2781 at 3270.797 kb on + strand, within Pf6N2E2_2781 at 3270.797 kb on + strand, within Pf6N2E2_2781 at 3270.797 kb on + strand, within Pf6N2E2_2781 at 3270.797 kb on + strand, within Pf6N2E2_2781 at 3270.797 kb on + strand, within Pf6N2E2_2781 at 3270.797 kb on + strand, within Pf6N2E2_2781 at 3270.797 kb on + strand, within Pf6N2E2_2781 at 3270.797 kb on + strand, within Pf6N2E2_2781 at 3270.797 kb on + strand, within Pf6N2E2_2781 at 3270.797 kb on + strand, within Pf6N2E2_2781 at 3270.797 kb on + strand, within Pf6N2E2_2781 at 3270.798 kb on - strand, within Pf6N2E2_2781 at 3270.798 kb on - strand, within Pf6N2E2_2781 at 3270.798 kb on - strand, within Pf6N2E2_2781 at 3270.798 kb on - strand, within Pf6N2E2_2781 at 3270.798 kb on - strand, within Pf6N2E2_2781 at 3270.798 kb on - strand, within Pf6N2E2_2781 at 3270.798 kb on - strand, within Pf6N2E2_2781 at 3270.798 kb on - strand, within Pf6N2E2_2781 at 3270.808 kb on - strand, within Pf6N2E2_2781 at 3271.035 kb on + strand, within Pf6N2E2_2781 at 3271.035 kb on + strand, within Pf6N2E2_2781 at 3271.036 kb on - strand, within Pf6N2E2_2781 at 3271.484 kb on - strand at 3271.603 kb on + strand at 3271.604 kb on - strand at 3271.604 kb on - strand at 3271.648 kb on + strand, within Pf6N2E2_2782 at 3271.685 kb on + strand, within Pf6N2E2_2782 at 3271.726 kb on + strand, within Pf6N2E2_2782 at 3271.727 kb on - strand, within Pf6N2E2_2782 at 3271.730 kb on + strand, within Pf6N2E2_2782 at 3271.730 kb on + strand, within Pf6N2E2_2782 at 3271.731 kb on - strand, within Pf6N2E2_2782 at 3272.136 kb on + strand, within Pf6N2E2_2782 at 3272.146 kb on + strand, within Pf6N2E2_2782 at 3272.359 kb on + strand, within Pf6N2E2_2782 at 3272.359 kb on + strand, within Pf6N2E2_2782 at 3272.359 kb on + strand, within Pf6N2E2_2782 at 3272.360 kb on - strand, within Pf6N2E2_2782 at 3272.385 kb on + strand, within Pf6N2E2_2782 at 3272.451 kb on - strand, within Pf6N2E2_2782 at 3272.481 kb on - strand, within Pf6N2E2_2782 at 3272.481 kb on - strand, within Pf6N2E2_2782 at 3272.559 kb on - strand, within Pf6N2E2_2782 at 3272.641 kb on + strand, within Pf6N2E2_2782 at 3272.641 kb on + strand, within Pf6N2E2_2782 at 3272.641 kb on + strand, within Pf6N2E2_2782 at 3272.641 kb on + strand, within Pf6N2E2_2782 at 3272.642 kb on - strand, within Pf6N2E2_2782 at 3272.642 kb on - strand, within Pf6N2E2_2782 at 3272.642 kb on - strand, within Pf6N2E2_2782 at 3272.642 kb on - strand, within Pf6N2E2_2782 at 3272.642 kb on - strand, within Pf6N2E2_2782 at 3272.669 kb on - strand, within Pf6N2E2_2782 at 3272.755 kb on + strand, within Pf6N2E2_2782 at 3272.755 kb on + strand, within Pf6N2E2_2782 at 3272.755 kb on + strand, within Pf6N2E2_2782 at 3272.756 kb on - strand, within Pf6N2E2_2782 at 3272.756 kb on - strand, within Pf6N2E2_2782 at 3272.945 kb on - strand, within Pf6N2E2_2782 at 3273.151 kb on + strand at 3273.151 kb on + strand at 3273.152 kb on - strand at 3273.202 kb on + strand at 3273.202 kb on + strand at 3273.202 kb on + strand at 3273.203 kb on - strand at 3273.203 kb on - strand at 3273.206 kb on + strand at 3273.206 kb on + strand at 3273.206 kb on + strand at 3273.206 kb on + strand at 3273.206 kb on + strand at 3273.206 kb on + strand at 3273.207 kb on - strand at 3273.207 kb on - strand at 3273.207 kb on - strand at 3273.207 kb on - strand at 3273.207 kb on - strand at 3273.207 kb on - strand at 3273.210 kb on + strand at 3273.210 kb on + strand at 3273.210 kb on + strand at 3273.211 kb on - strand at 3273.220 kb on + strand at 3273.220 kb on + strand at 3273.220 kb on + strand at 3273.220 kb on + strand at 3273.221 kb on - strand at 3273.247 kb on + strand at 3273.291 kb on + strand at 3273.291 kb on + strand at 3273.291 kb on + strand at 3273.291 kb on + strand at 3273.292 kb on - strand at 3273.309 kb on - strand at 3273.309 kb on - strand at 3273.338 kb on + strand at 3273.460 kb on - strand at 3273.460 kb on - strand at 3273.460 kb on - strand at 3273.534 kb on + strand, within Pf6N2E2_2783 at 3273.535 kb on - strand, within Pf6N2E2_2783 at 3273.537 kb on + strand, within Pf6N2E2_2783 at 3273.537 kb on + strand, within Pf6N2E2_2783 at 3273.582 kb on + strand, within Pf6N2E2_2783 at 3273.582 kb on + strand, within Pf6N2E2_2783 at 3273.582 kb on + strand, within Pf6N2E2_2783 at 3273.583 kb on - strand, within Pf6N2E2_2783 at 3273.583 kb on - strand, within Pf6N2E2_2783 at 3273.583 kb on - strand, within Pf6N2E2_2783 at 3273.583 kb on - strand, within Pf6N2E2_2783 at 3273.604 kb on + strand, within Pf6N2E2_2783 at 3273.604 kb on + strand, within Pf6N2E2_2783 at 3273.605 kb on - strand, within Pf6N2E2_2783 at 3273.605 kb on - strand, within Pf6N2E2_2783 at 3273.654 kb on + strand, within Pf6N2E2_2783 at 3273.655 kb on - strand, within Pf6N2E2_2783 at 3273.655 kb on - strand, within Pf6N2E2_2783 at 3273.655 kb on - strand, within Pf6N2E2_2783 at 3274.011 kb on + strand, within Pf6N2E2_2783 at 3274.011 kb on + strand, within Pf6N2E2_2783 at 3274.012 kb on - strand, within Pf6N2E2_2783 at 3274.012 kb on - strand, within Pf6N2E2_2783 at 3274.176 kb on + strand at 3274.177 kb on - strand at 3274.177 kb on - strand at 3274.183 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Urea (N) remove 3,270,538 - Pf6N2E2_2781 0.76 +0.3 3,270,654 + Pf6N2E2_2781 0.79 +0.8 3,270,655 - Pf6N2E2_2781 0.79 +0.3 3,270,682 - Pf6N2E2_2781 0.80 -0.3 3,270,741 + Pf6N2E2_2781 0.81 +0.3 3,270,741 + Pf6N2E2_2781 0.81 +0.3 3,270,741 + Pf6N2E2_2781 0.81 -0.5 3,270,741 + Pf6N2E2_2781 0.81 -0.5 3,270,741 + Pf6N2E2_2781 0.81 -0.1 3,270,742 - Pf6N2E2_2781 0.82 -0.2 3,270,742 - Pf6N2E2_2781 0.82 +0.2 3,270,742 - Pf6N2E2_2781 0.82 -1.2 3,270,742 - Pf6N2E2_2781 0.82 +0.2 3,270,742 - Pf6N2E2_2781 0.82 +0.3 3,270,742 - Pf6N2E2_2781 0.82 -0.9 3,270,742 - Pf6N2E2_2781 0.82 -0.2 3,270,742 - Pf6N2E2_2781 0.82 +0.1 3,270,795 + Pf6N2E2_2781 0.83 -0.2 3,270,795 + Pf6N2E2_2781 0.83 +0.8 3,270,795 + Pf6N2E2_2781 0.83 -0.7 3,270,795 + Pf6N2E2_2781 0.83 +0.5 3,270,796 - Pf6N2E2_2781 0.83 -0.7 3,270,796 - Pf6N2E2_2781 0.83 +1.0 3,270,796 - Pf6N2E2_2781 0.83 -1.1 3,270,796 - Pf6N2E2_2781 0.83 +0.4 3,270,796 - Pf6N2E2_2781 0.83 +0.6 3,270,796 - Pf6N2E2_2781 0.83 +0.1 3,270,796 - Pf6N2E2_2781 0.83 +0.3 3,270,796 - Pf6N2E2_2781 0.83 -0.5 3,270,796 - Pf6N2E2_2781 0.83 -1.0 3,270,796 - Pf6N2E2_2781 0.83 +0.8 3,270,796 - Pf6N2E2_2781 0.83 +0.2 3,270,796 - Pf6N2E2_2781 0.83 +0.1 3,270,797 + Pf6N2E2_2781 0.83 -1.1 3,270,797 + Pf6N2E2_2781 0.83 -0.0 3,270,797 + Pf6N2E2_2781 0.83 +2.4 3,270,797 + Pf6N2E2_2781 0.83 -0.1 3,270,797 + Pf6N2E2_2781 0.83 -0.3 3,270,797 + Pf6N2E2_2781 0.83 -0.8 3,270,797 + Pf6N2E2_2781 0.83 -0.1 3,270,797 + Pf6N2E2_2781 0.83 +0.2 3,270,797 + Pf6N2E2_2781 0.83 -0.9 3,270,797 + Pf6N2E2_2781 0.83 -0.9 3,270,797 + Pf6N2E2_2781 0.83 -0.1 3,270,797 + Pf6N2E2_2781 0.83 -0.8 3,270,797 + Pf6N2E2_2781 0.83 -1.1 3,270,797 + Pf6N2E2_2781 0.83 +0.0 3,270,797 + Pf6N2E2_2781 0.83 -1.5 3,270,798 - Pf6N2E2_2781 0.83 -1.7 3,270,798 - Pf6N2E2_2781 0.83 +0.4 3,270,798 - Pf6N2E2_2781 0.83 -0.4 3,270,798 - Pf6N2E2_2781 0.83 +0.8 3,270,798 - Pf6N2E2_2781 0.83 +0.1 3,270,798 - Pf6N2E2_2781 0.83 +0.1 3,270,798 - Pf6N2E2_2781 0.83 -0.4 3,270,798 - Pf6N2E2_2781 0.83 -0.9 3,270,808 - Pf6N2E2_2781 0.83 +1.6 3,271,035 + Pf6N2E2_2781 0.89 -0.6 3,271,035 + Pf6N2E2_2781 0.89 -0.4 3,271,036 - Pf6N2E2_2781 0.90 -0.5 3,271,484 - +0.5 3,271,603 + -0.1 3,271,604 - +0.7 3,271,604 - -0.3 3,271,648 + Pf6N2E2_2782 0.11 +0.0 3,271,685 + Pf6N2E2_2782 0.14 -0.6 3,271,726 + Pf6N2E2_2782 0.16 +0.7 3,271,727 - Pf6N2E2_2782 0.16 -0.7 3,271,730 + Pf6N2E2_2782 0.16 +0.7 3,271,730 + Pf6N2E2_2782 0.16 -0.5 3,271,731 - Pf6N2E2_2782 0.16 +0.9 3,272,136 + Pf6N2E2_2782 0.39 +0.8 3,272,146 + Pf6N2E2_2782 0.39 -1.1 3,272,359 + Pf6N2E2_2782 0.51 +0.7 3,272,359 + Pf6N2E2_2782 0.51 -0.3 3,272,359 + Pf6N2E2_2782 0.51 -1.1 3,272,360 - Pf6N2E2_2782 0.51 -0.3 3,272,385 + Pf6N2E2_2782 0.53 +0.9 3,272,451 - Pf6N2E2_2782 0.57 +0.3 3,272,481 - Pf6N2E2_2782 0.58 -0.4 3,272,481 - Pf6N2E2_2782 0.58 -1.2 3,272,559 - Pf6N2E2_2782 0.63 -0.2 3,272,641 + Pf6N2E2_2782 0.67 -0.2 3,272,641 + Pf6N2E2_2782 0.67 +0.2 3,272,641 + Pf6N2E2_2782 0.67 -0.0 3,272,641 + Pf6N2E2_2782 0.67 -0.2 3,272,642 - Pf6N2E2_2782 0.67 -0.0 3,272,642 - Pf6N2E2_2782 0.67 +0.4 3,272,642 - Pf6N2E2_2782 0.67 +1.6 3,272,642 - Pf6N2E2_2782 0.67 +0.7 3,272,642 - Pf6N2E2_2782 0.67 -0.4 3,272,669 - Pf6N2E2_2782 0.69 +0.9 3,272,755 + Pf6N2E2_2782 0.74 +0.6 3,272,755 + Pf6N2E2_2782 0.74 -0.2 3,272,755 + Pf6N2E2_2782 0.74 -0.6 3,272,756 - Pf6N2E2_2782 0.74 -0.2 3,272,756 - Pf6N2E2_2782 0.74 +0.3 3,272,945 - Pf6N2E2_2782 0.84 +1.0 3,273,151 + -1.4 3,273,151 + -0.0 3,273,152 - +0.1 3,273,202 + +0.2 3,273,202 + +0.0 3,273,202 + +0.3 3,273,203 - +0.1 3,273,203 - -0.2 3,273,206 + +0.5 3,273,206 + +0.2 3,273,206 + -0.3 3,273,206 + +0.6 3,273,206 + -0.4 3,273,206 + -0.6 3,273,207 - -0.0 3,273,207 - -0.4 3,273,207 - -0.4 3,273,207 - -1.4 3,273,207 - -0.4 3,273,207 - +0.1 3,273,210 + -1.4 3,273,210 + -0.2 3,273,210 + -0.7 3,273,211 - +0.1 3,273,220 + -0.4 3,273,220 + -0.1 3,273,220 + +0.7 3,273,220 + +0.6 3,273,221 - +1.0 3,273,247 + +0.2 3,273,291 + -0.7 3,273,291 + -0.2 3,273,291 + +0.7 3,273,291 + -1.0 3,273,292 - +0.1 3,273,309 - +1.8 3,273,309 - -0.6 3,273,338 + -0.6 3,273,460 - +0.7 3,273,460 - +0.8 3,273,460 - +0.3 3,273,534 + Pf6N2E2_2783 0.13 +1.8 3,273,535 - Pf6N2E2_2783 0.13 +0.4 3,273,537 + Pf6N2E2_2783 0.13 +0.3 3,273,537 + Pf6N2E2_2783 0.13 -0.9 3,273,582 + Pf6N2E2_2783 0.19 -1.2 3,273,582 + Pf6N2E2_2783 0.19 +0.6 3,273,582 + Pf6N2E2_2783 0.19 -0.1 3,273,583 - Pf6N2E2_2783 0.19 -0.4 3,273,583 - Pf6N2E2_2783 0.19 -0.2 3,273,583 - Pf6N2E2_2783 0.19 +0.4 3,273,583 - Pf6N2E2_2783 0.19 -0.2 3,273,604 + Pf6N2E2_2783 0.22 -0.5 3,273,604 + Pf6N2E2_2783 0.22 +0.5 3,273,605 - Pf6N2E2_2783 0.22 -0.5 3,273,605 - Pf6N2E2_2783 0.22 -0.4 3,273,654 + Pf6N2E2_2783 0.29 +0.9 3,273,655 - Pf6N2E2_2783 0.29 +0.4 3,273,655 - Pf6N2E2_2783 0.29 +0.4 3,273,655 - Pf6N2E2_2783 0.29 -0.7 3,274,011 + Pf6N2E2_2783 0.77 +0.5 3,274,011 + Pf6N2E2_2783 0.77 +1.5 3,274,012 - Pf6N2E2_2783 0.77 -0.0 3,274,012 - Pf6N2E2_2783 0.77 +0.5 3,274,176 + +1.8 3,274,177 - -1.3 3,274,177 - -0.7 3,274,183 - -1.2
Or see this region's nucleotide sequence